Entry Name N-isopropylammelide isopropylaminohydrolase;
Class Hydrolases;BRITE hierarchy
Sysname N-isopropylammelide isopropylaminohydrolase
Reaction(IUBMB) N-isopropylammelide + H2O = cyanuric acid + isopropylamine [RN:
R05560 ]
Reaction(KEGG) Substrate Product Comment Requires Zn2+. This bacterial enzyme is involved in degradation of the herbicide atrazine. It can hydrolyse other N-substituted amino dihydroxy-s-triazine molecules, and prefers substrates with linear N-alkyl groups to those with branched alkyl groups.
History EC 3.5.4.42 created 2000 as EC 3.5.99.4, transferred 2016 to EC 3.5.4.42
Pathway ec01120 Microbial metabolism in diverse environments
Orthology K08710 N-isopropylammelide isopropylaminohydrolase
Genes Taxonomy Reference Authors Sadowsky MJ, Tong Z, de Souza M, Wackett LP
Title AtzC is a new member of the amidohydrolase protein superfamily and is homologous to other atrazine-metabolizing enzymes.
Journal Sequence Reference Authors Shapir N, Osborne JP, Johnson G, Sadowsky MJ, Wackett LP
Title Purification, substrate range, and metal center of AtzC: the N-isopropylammelide aminohydrolase involved in bacterial atrazine metabolism.
Journal Reference Authors Balotra S, Warden AC, Newman J, Briggs LJ, Scott C, Peat TS
Title X-Ray Structure and Mutagenesis Studies of the N-Isopropylammelide Isopropylaminohydrolase, AtzC.
Journal Sequence Other DBs ExplorEnz - The Enzyme Database: 3.5.4.42
ExPASy - ENZYME nomenclature database: 3.5.4.42
