Entry
Name
hydroxydechloroatrazine ethylaminohydrolase;
atzB (gene name);
2,4-dihydroxy-6-(isopropylamino)-1,3,5-triazine ethylaminohydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amidines
BRITE hierarchy
Sysname
hydroxyatrazine ethylaminohydrolase
Reaction(IUBMB)
hydroxyatrazine + H2O = N-isopropylammelide + ethylamine [RN:
R05559 ]
Reaction(KEGG)
Substrate
Product
Comment
Contains Zn2+. This bacterial enzyme is involved in degradation of the herbicide atrazine. The enzyme has a broad substrate range, and requires a monohydroxylated s-triazine ring with a minimum of one primary or secondary amine substituent and either a chloride or amine leaving group. It catalyses both deamination and dechlorination reactions.
History
EC 3.5.4.43 created 2000 as EC 3.5.99.3, transferred 2016 to EC 3.5.4.43
Pathway
ec01120 Microbial metabolism in diverse environments
Orthology
K03382 hydroxydechloroatrazine ethylaminohydrolase
Genes
ENU : PYH37_001504 PYH37_006390
REL : REMIM1_PB00101(atzB)
REP : IE4803_PD00305(atzB)
REI : IE4771_PE00321(atzB)
RHL : LPU83_pLPU83d0291(atzB)
RGA : RGR602_PC00659(atzB)
» show all
Taxonomy
Reference
Authors
Boundy-Mills KL, de Souza ML, Mandelbaum RT, Wackett LP, Sadowsky MJ
Title
The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway.
Journal
Sequence
Reference
Authors
Seffernick JL, Aleem A, Osborne JP, Johnson G, Sadowsky MJ, Wackett LP
Title
Hydroxyatrazine N-ethylaminohydrolase (AtzB): an amidohydrolase superfamily enzyme catalyzing deamination and dechlorination.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.4.43
ExPASy - ENZYME nomenclature database: 3.5.4.43