KEGG ENZYME: 4.1.1.119

ENZYME: 4.1.1.119

Entry

EC 4.1.1.119                Enzyme

Name

phenylacetate decarboxylase;
phdB (gene name)

Class

Lyases;
Carbon-carbon lyases;
Carboxy-lyases

Sysname

phenylacetate carboxy-lyase

Reaction(IUBMB)

phenylacetate = toluene + CO2 [RN:R12504]

Reaction(KEGG)

R12504

Substrate

phenylacetate [CPD:C07086]

Product

toluene [CPD:C01455];
CO2 [CPD:C00011]

Comment

This bacterial enzyme, isolated from anoxic, toluene-producing microbial communities, is a glycyl radical enzyme. It needs to be activated by a dedicated activating enzyme (PhdA). The activase catalyses the reductive cleavage of AdoMet, producing a 5'-deoxyadenosyl radical that leads to the production of the glycyl radical in PhdB.

History

EC 4.1.1.119 created 2019

Reference

1 [PMID:27506494]

Authors

Zargar K, Saville R, Phelan RM, Tringe SG, Petzold CJ, Keasling JD, Beller HR

Title

In vitro Characterization of Phenylacetate Decarboxylase, a Novel Enzyme Catalyzing Toluene Biosynthesis in an Anaerobic Microbial Community.

Journal

Sci Rep 6:31362 (2016)
DOI:10.1038/srep31362

Reference

2 [PMID:29556105]

Authors

Beller HR, Rodrigues AV, Zargar K, Wu YW, Saini AK, Saville RM, Pereira JH, Adams PD, Tringe SG, Petzold CJ, Keasling JD

Title

Discovery of enzymes for toluene synthesis from anoxic microbial communities.

Journal

Nat Chem Biol 14:451-457 (2018)
DOI:10.1038/s41589-018-0017-4

Sequence

[ag:PSH03431]

Reference

3 [PMID:31512343]

Authors

Rodrigues AV, Tantillo DJ, Mukhopadhyay A, Keasling JD, Beller HR.

Title

Insight into the Mechanism of Phenylacetate Decarboxylase (PhdB), a Toluene-Producing Glycyl Radical Enzyme.

Journal

Chembiochem 21:663-671 (2020)
DOI:10.1002/cbic.201900560

Other DBs

ExplorEnz - The Enzyme Database:

4.1.1.119

IUBMB Enzyme Nomenclature:

4.1.1.119

ExPASy - ENZYME nomenclature database:

4.1.1.119

BRENDA, the Enzyme Database:

4.1.1.119

All links

Chemical substance (3)   
   KEGG COMPOUND (3)   
Chemical reaction (1)   
   KEGG REACTION (1)   
All databases (4)   

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