KEGG ENZYME: 4.1.2.45

ENZYME: 4.1.2.45

Entry

EC 4.1.2.45                 Enzyme

Name

trans-o-hydroxybenzylidenepyruvate hydratase-aldolase;
2'-hydroxybenzalpyruvate aldolase;
NsaE;
tHBPA hydratase-aldolase

Class

Lyases;
Carbon-carbon lyases;
Aldehyde-lyases

Sysname

(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate hydro-lyase

Reaction(IUBMB)

(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O = salicylaldehyde + pyruvate [RN:R05136]

Reaction(KEGG)

R05136

Substrate

(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate [CPD:C06203];
H2O [CPD:C00001]

Product

salicylaldehyde [CPD:C06202];
pyruvate [CPD:C00022]

Comment

This enzyme is involved in naphthalene degradation. The enzyme catalyses a retro-aldol reaction in vitro, and it accepts a broad range of aldehydes and 4-substituted 2-oxobut-3-enoates as substrates [4].

History

EC 4.1.2.45 created 2010, modified 2011

Pathway

ec00626

Naphthalene degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K14585

trans-o-hydroxybenzylidenepyruvate hydratase-aldolase

Genes

PPI:	ND061(nahE)

PFK:	PFAS1_28640

PSC:	A458_06700

PSTU:

UIB01_22650

PBM:	CL52_14635

ALT:	ambt_17910

CYQ:

Q91_1157

CZA:	CYCME_1270

CYY:	CPC19_06820

FCE:	JN531_005675

GBI:	PG2T_00740 PG2T_05760 PG2T_12860

RMN:	TK49_07980

BMK:	DM80_1449(phdJ)

BUQ:	AC233_10870 AC233_11110

BFN:	OI25_3035 OI25_3053(phdJ)

PARB:

CJU94_38635

PNA:

Pnap_2479

ACID:

CBP33_05390

ACIP:

CBP36_05840

ACIN:

CBP34_05225

ACIS:

CBP35_13105

DEL:	DelCs14_1760

MASW:

AM586_27480

RBU:	PG1C_04265 PG1C_08125

NPM:	QEO92_31580

MAAD:

AZF01_18315

CID:

P73_0351

CON:	TQ29_19110

PGV:	SL003B_4082 SL003B_4105

NAR:

Saro_3836

NPP:	PP1Y_AT15847 PP1Y_AT31250

NPN:	JI59_00960 JI59_04200 JI59_21720 JI59_21740 JI59_21760 JI59_21850 JI59_21910 JI59_24690

NOG:	GKE62_05260

SBAR:

H5V43_22420

SPHY:

CHN51_07055

SPHJ:

BSL82_13970

CNA:	AB433_00715 AB433_10235 AB433_10410 AB433_18070

ERY:	CP97_14265

MMAN:

MMAN_22710

SBAT:

G4Z16_01255

NMAR:

HPC71_10555

» show all

Reference

1 [PMID:8486638]

Authors

Kuhm AE, Knackmuss HJ, Stolz A

Title

Purification and properties of 2'-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates.

Journal

J Biol Chem 268:9484-9 (1993)

Reference

2 [PMID:16804169]

Authors

Keck A, Conradt D, Mahler A, Stolz A, Mattes R, Klein J

Title

Identification and functional analysis of the genes for naphthalenesulfonate catabolism by Sphingomonas xenophaga BN6.

Journal

Microbiology 152:1929-40 (2006)
DOI:10.1099/mic.0.28783-0

Sequence

[ag:AAD45417]

Reference

3 [PMID:8002605]

Authors

Eaton RW

Title

Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid.

Journal

J Bacteriol 176:7757-62 (1994)
DOI:10.1128/JB.176.24.7757-7762.1994

Sequence

[ag:AAA66357]

Reference

4 [PMID:10831455]

Authors

Eaton RW

Title

trans-o-Hydroxybenzylidenepyruvate hydratase-aldolase as a biocatalyst.

Journal

Appl Environ Microbiol 66:2668-72 (2000)
DOI:10.1128/AEM.66.6.2668-2672.2000