KEGG   ENZYME: 4.1.2.65
Entry
EC 4.1.2.65                 Enzyme                                 
Name
ferulate hydratase/lyase;
vanillin synthase;
VpVan;
VAN;
ferulate aldolase
Class
Lyases;
Carbon-carbon lyases;
Aldehyde-lyases
Sysname
ferulate acetate-lyase (vanillin-forming)
Reaction(IUBMB)
ferulate + H2O = vanillin + acetate (overall reaction);
(1a) ferulate + H2O = 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoate;
(1b) 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoate = vanillin + acetate
Substrate
ferulate [CPD:C01494];
H2O [CPD:C00001];
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoate
Product
vanillin [CPD:C00755];
acetate [CPD:C00033];
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoate
Comment
The enzyme is located in the chloroplasts of vanilla pods of the orchid Vanilla planifolia. It also converts ferulic acid 4-O-beta-D-glucopyranoside to vanillin 4-O-beta-D-glucopyranoside.
History
EC 4.1.2.65 created 2024
Reference
1  [PMID:24941968]
  Authors
Gallage NJ, Hansen EH, Kannangara R, Olsen CE, Motawia MS, Jorgensen K, Holme I, Hebelstrup K, Grisoni M, Moller BL.
  Title
Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme.
  Journal
Nat Commun 5:4037 (2014)
DOI:10.1038/ncomms5037
  Sequence
[ag:AKG47593]
Reference
2  [PMID:28357540]
  Authors
Kundu A.
  Title
Vanillin biosynthetic pathways in plants.
  Journal
Planta 245:1069-1078 (2017)
DOI:10.1007/s00425-017-2684-x
Reference
3  [PMID:29186560]
  Authors
Gallage NJ, Jorgensen K, Janfelt C, Nielsen AJZ, Naake T, Dunski E, Dalsten L, Grisoni M, Moller BL.
  Title
The Intracellular Localization of the Vanillin Biosynthetic Machinery in Pods of Vanilla planifolia.
  Journal
Plant Cell Physiol 59:304-318 (2018)
DOI:10.1093/pcp/pcx185
  Sequence
[ag:AKG47593]
Other DBs
ExplorEnz - The Enzyme Database: 4.1.2.65
IUBMB Enzyme Nomenclature: 4.1.2.65
ExPASy - ENZYME nomenclature database: 4.1.2.65
BRENDA, the Enzyme Database: 4.1.2.65

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