KEGG ENZYME: 4.1.3.43

ENZYME: 4.1.3.43

Entry

EC 4.1.3.43                 Enzyme

Name

4-hydroxy-2-oxohexanoate aldolase;
BphI

Class

Lyases;
Carbon-carbon lyases;
Oxo-acid-lyases

Sysname

(S)-4-hydroxy-2-oxohexanoate pyruvate-lyase (propanal-forming)

Reaction(IUBMB)

(S)-4-hydroxy-2-oxohexanoate = propanal + pyruvate

Reaction(KEGG)

(other) R05298

Substrate

(S)-4-hydroxy-2-oxohexanoate

Product

propanal [CPD:C00479];
pyruvate [CPD:C00022]

Comment

Requires Mn2+ for maximal activity [1,2]. The enzymes from the bacteria Burkholderia xenovorans and Thermus thermophilus also perform the reaction of EC 4.1.3.39, 4-hydroxy-2-oxovalerate aldolase [1,2,6]. The enzyme forms a bifunctional complex with EC 1.2.1.87, propanal dehydrogenase (CoA-propanoylating), with a tight channel connecting the two subunits [3,4,6].

History

EC 4.1.3.43 created 2013

Pathway

ec00622

Xylene degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K18365

4-hydroxy-2-oxovalerate/4-hydroxy-2-oxohexanoate aldolase

Genes

PSD:

DSC_09375

RGL:	CS053_01770(dmpG)

RTH:	LRK53_10290(dmpG)

RBD:	ALSL_0877 ALSL_1082

VAF:	D1115_22440(dmpG)

PRE:	PCA10_13840

PFUW:

KF707C_12190 KF707C_16050(salH) KF707C_16230(bphX3)

PPI:	ND084(nahM)

PPUT:

L483_14055

PPUN:

PP4_27280(mhpE)

PVR:	PverR02_11585 PverR02_14710

PPUU:

PputUW4_01662(mhpE)

PKC:	PKB_4237(nahM)

PFZ:	AV641_10525

PALK:

PSAKL28_05980

PPSY:

AOC04_07825

PFK:	PFAS1_28765

PSET:

THL1_1435

PSIL:

PMA3_11725

PKE:	DLD99_14740(dmpG)

PALL:

UYA_06970 UYA_07050

PUM:	HGP31_20770(dmpG)

PPSH:

G5J76_13480(dmpG)

PLAL:

FXN65_06430(dmpG)

PAZE:

KSS91_19285(dmpG)

PTAE:

NCTC10697_02055(bphI)

PCAV:

D3880_17515(dmpG)

PPHN:

HU825_04960(dmpG) HU825_14765(dmpG)

PIZ:	LAB08_R40950(dmpG)

PKM:	PZ739_11535(dmpG)

PSC:	A458_06510

PSTU:

UIB01_22750

PBM:	CL52_14720

SDEG:

GOM96_01770(dmpG)

AVN:	Avin_30570(lapG)

AVL:	AvCA_30570(lapG)

AVD:	AvCA6_30570(lapG)

HARR:

HV822_17985(dmpG)

PPHA:

BVH74_17970

MARJ:

MARI_05460(bphI)

MSHE:

MAALD49_03450(mhpE)

KAK:	Kalk_07865(dmpG)

ALC:	OTEC02_08820

AEI:	AOY20_01975

ASJ:	AsACE_CH00352(nahM)

ADV:	DJ533_16960(dmpG)

ARJ:	DOM24_01575(dmpG)

AWU:	BEN71_02690(dmpG)

ACUM:

C9E88_006015(dmpG)

ALW:	FOB21_11710(dmpG)

ATN:	FM020_13980(dmpG)

ACHI:

CDG60_02965(dmpG)

ALJ:	G8D99_13400(dmpG)

AWG:	GFH30_01715(dmpG)

ASHA:

G8E00_02255(dmpG)

ATG:	J4G44_04390(dmpG)

AVB:	RYU24_27150(mhpE)

ABOU:

ACBO_27740(mhpE_2)

ACIU:

A3K93_01265

APOT:

MZO21_10840(dmpG)

ATZ:	M5E07_14190(dmpG)

ACIE:

KIP84_08190(dmpG)

SPL:

Spea_2116

SHL:

Shal_2088

SWD:

Swoo_1390

COLW:

A3Q33_18585

CBER:

B5D82_11260(dmpG)

THT:	E2K93_11210(dmpG)

PHA:	PSHAa2143(mhpE)

PTN:	PTRA_a2596(bphI)

PNG:	PNIG_a2790(bphI)

LAL:	AT746_01290

SALH:

HMF8227_02732(bphI)

TTU:	TERTU_1283(dmpG)

SPOI:

IMCC21906_00395 IMCC21906_02714

SNAN:

I6N98_10790(dmpG)

STAW:

NCG89_14280(dmpG)

ZAL:	AZF00_03870 AZF00_11420 AZF00_17810

HJA:	BST95_09515

HALC:

EY643_13285(dmpG)

AFUS:

EYZ66_02685(dmpG) EYZ66_12975(dmpG)

PAQM:

E0F26_07465(dmpG)

CYQ:	Q91_0510 Q91_1491(lapG)

CZA:	CYCME_0969(lapG) CYCME_2101(dmpG)

CYY:	CPC19_05395(dmpG) CPC19_10505(dmpG)

BLEP:

AL038_01290(dmpG)

THIS:

HZT40_10615(dmpG)

AEH:

Mlg_2462

TBN:

TBH_C0930

MMW:	Mmwyl1_3801

MPRI:

MP3633_3149(mhpE)

MGEO:

CFI10_00435(dmpG) CFI10_04970(dmpG) CFI10_06050(dmpG)

MSEC:

LN244_04515(dmpG) LN244_04765(dmpG) LN244_11320(dmpG)

MRZ:	KDW95_00755(dmpG)

NCU:	F0U83_03055(dmpG)

NJP:	NEJAP_0673(mhpE)

NIK:	F5I99_02020(dmpG) F5I99_10095(dmpG) F5I99_10510(dmpG)

AJP:	AMJAP_2526 AMJAP_2997

ENW:	MJO57_21390(dmpG)

EMP:	EZMO1_1319(tesG)

ASIM:

FE240_03470(dmpG)

ZDF:	AN401_14615

SOK:	D0B54_06190(dmpG)

SINI:

GT972_14660(dmpG)

FCE:	JN531_005840(dmpG)

GBI:	PG2T_09675

GPB:	HDN1F_27070(tesG)

NZO:	SAMEA4504057_0444(bphI)

NCI:	NCTC10296_00917(bphI)

NWD:	H3L96_04775(dmpG)

NDU:	LVJ88_00580(dmpG)

VFF:	VITFI_CDS1376

PTES:

JQU52_04850(dmpG)

USU:	LVJ78_03650(dmpG)

AQL:	BXU06_16260 BXU06_16885 BXU06_17065

RPI:

Rpic_4617

RPF:	Rpic12D_3541

RIN:	ACS15_4356

REH:	H16_B0595(bpHI)

CNC:	CNE_2c05400(bpHI)

CUH:	BJN34_24650

RME:	Rmet_1319(bphI)

CTI:	RALTA_B0399(mhpE)

CBW:	RR42_s2315

COX:	E0W60_08015(dmpG)

CCAX:

KZ686_10005(dmpG)

CUK:	KB879_20415(dmpG)

BVI:	Bcep1808_7605

BUR:	Bcep18194_B1457 Bcep18194_B2959

BCJ:	BCAM1615(bphI)

BCEN:

DM39_3381(dmpG) DM39_4832(dmpG)

BCEO:

I35_5475(bphI)

BCT:	GEM_4109 GEM_5401

BCED:

DM42_4991(dmpG) DM42_6343(dmpG)

BCEP:

APZ15_31145

BPYR:

ABD05_27810

BCON:

NL30_04130 NL30_24590

BDF:	WI26_15995 WI26_21155

BTEI:

WS51_00570 WS51_05705

BPSL:

WS57_06035 WS57_12155

BMEC:

WJ16_26800

BSTL:

BBJ41_10520 BBJ41_21350

BANN:

JFN94_19135(dmpG) JFN94_25660(dmpG)

BARI:

NLX30_30175(dmpG)

BAEN:

L3V59_21750(dmpG) L3V59_30405(dmpG)

BUQ:	AC233_11185

BURK:

DM992_32615(dmpG)

BXE:	Bxe_C1187(bphI)

BXB:	DR64_8618(dmpG)

BFN:	OI25_4591(dmpG)

PARB:

CJU94_37880(dmpG) CJU94_38125(dmpG)

PCAF:

DSC91_003289

PMEG:

FNZ07_21155(dmpG)

PBRY:

NDK50_32885(dmpG)

PPAI:

E1956_37230(dmpG)

PPK:	U875_01890

PRB:	X636_07580

CABA:

SBC2_49000(bphI)

LIMN:

HKT17_03830(dmpG)

AXY:	AXYL_04307(dmpG)

APES:

FOC84_00305(dmpG)

AALN:

Q3V95_01645(dmpG)

CDN:	BN940_14431

RFR:

Rfer_0454

PNA:

Pnap_4142

AJS:

Ajs_0224

ACID:

CBP33_01115

ACIP:

CBP36_01210

ACIN:

CBP34_01120

ACIS:

CBP35_17740

AMON:

H9L24_14175(dmpG)

VEI:

Veis_2781

VBO:	CKY39_28495(dmpG)

CTT:	CtCNB1_1351 CtCNB1_3147

CTES:

O987_07730

COF:	FOZ74_08315(dmpG)

CRJ:	QMY55_07010(dmpG)

ADN:	Alide_0339

ADK:	Alide2_0284

OTD:	J1M35_00045(dmpG)

HYL:	LPB072_18200

HPSE:

HPF_01850(bphI)

HYN:	F9K07_02775(dmpG) F9K07_11450(dmpG) F9K07_31030(dmpG)

HTN:	KI616_01945(dmpG)

DIH:	G7047_09695(dmpG)

SIMP:

C6571_13625(dmpG)

MELA:

C6568_07850(dmpG)

MJE:	LVC68_01015(dmpG)

STHM:

IS481_10550(dmpG)

SAQA:

OMP39_09130(dmpG)

MPT:	Mpe_A2266 Mpe_A3321

METP:

C1M51_06440(dmpG) C1M51_16320(dmpG)

SNN:	EWH46_19310(dmpG)

IDC:	LRM40_19585(dmpG)

LCH:

Lcho_3340

JAJ:	EKL02_01800(dmpG) EKL02_13150(dmpG)

MASW:

AM586_18660

TMJ:	P0M04_30655(dmpG)

RBU:	PG1C_14090

ABRE:

pbN1_20580(mhpE)

DAR:	Daro_0907 Daro_1356 Daro_3782 Daro_3808

DEY:	HYN24_14110(dmpG) HYN24_14200(dmpG)

DDEN:

KI615_16870(dmpG)

QAU:	KI612_15145(dmpG)

FLE:	KI610_08595(dmpG) KI610_13435(dmpG) KI610_15450(dmpG)

AZO:	azo1857(lapG) azo1972(mhpE) azo2431(nahM)

AOA:	dqs_2010 dqs_2127 dqs_2575

AZI:	AzCIB_1509 AzCIB_4409

AZD:	CDA09_09965(dmpG) CDA09_10335(dmpG) CDA09_10460(dmpG) CDA09_21430(dmpG)

AZR:	CJ010_12270(dmpG) CJ010_12835(dmpG) CJ010_15205(dmpG)

TMZ:	Tmz1t_2079 Tmz1t_3105

THU:	AC731_001820 AC731_008905

THAU:

C4PIVTH_2809(mhpE) C4PIVTH_4319(mhpE) C4PIVTH_4382(mhpE)

THK:	CCZ27_04605(dmpG) CCZ27_10750(dmpG)

AZQ:	G3580_00550(dmpG) G3580_08280(dmpG)

ACOM:

CEW83_03610(dmpG)

NPM:	QEO92_31660(dmpG)

SHZ:

shn_33310

CID:

P73_1382

DAA:	AKL17_3p0139

TEC:	AKL02_008270(dmpG)

PGV:	SL003B_p0059

NPP:	PP1Y_AT32722

SGI:	SGRAN_1579(mhpE)

SJP:	SJA_C1-11910(mhpE_xylK)

SINB:

SIDU_08310

SYB:	TZ53_09030 TZ53_23475

SHYD:

CJD35_06515(dmpG)

SBAR:

H5V43_20635(dmpG)

SFLA:

SPHFLASMR4Y_02366(bphI)

SPHJ:

BSL82_10435

PMAS:

NCF86_10605(dmpG)

TFV:	IDJ81_04960(dmpG)

TXI:

TH3_21418

HTL:

HPTL_0047

ACIB:

ACBT_0259(dmpG)

AFC:	AFAEC_1844(dmpG)

AAQI:

AAQM_0982(dmpG)

ACLO:

ACLO_a0139(dmpG)

ADZ:	ADFLV_2551(dmpG)

ARC:

ABLL_2442

AROS:

NJU99_01650(dmpG)

ALK:	ALEK_0383(dmpG)

AANA:

AANAER_1260(dmpG)

GYC:	GYMC61_2298

GYA:	GYMC52_1426

GCT:	GC56T3_2055

GGH:	GHH_c14840(pheE)

GJF:	M493_17920

AAD:	TC41_0802(mhpE)

AACO:

K1I37_06160(dmpG)

ADAU:

NZD86_00705(dmpG) NZD86_06135(dmpG)

AFAS:

NZD89_10110(dmpG)

BTS:

Btus_0969

KYR:	CVV65_10345(dmpG) CVV65_11290(dmpG)

DHD:

Dhaf_1245

DCA:	Desca_1927

LACY:

A4V08_07730

MTHO:

MOTHE_c17890

MTHZ:

MOTHA_c18720

TOC:

Toce_0378

STED:

SPTER_03000 SPTER_36960(bphI)

SSM:	Spirs_4175

NAJ:	B1756_14020 B1756_15945

» show all

Reference

1 [PMID:19476337]

Authors

Baker P, Pan D, Carere J, Rossi A, Wang W, Seah SY

Title

Characterization of an aldolase-dehydrogenase complex that exhibits substrate channeling in the polychlorinated biphenyls degradation pathway.

Journal

Biochemistry 48:6551-8 (2009)
DOI:10.1021/bi9006644

Sequence

[bxe:Bxe_C1187]

Reference

2 [PMID:20364820]

Authors

Wang W, Baker P, Seah SY

Title

Comparison of two metal-dependent pyruvate aldolases related by convergent evolution: substrate specificity, kinetic mechanism, and substrate channeling.

Journal

Biochemistry 49:3774-82 (2010)
DOI:10.1021/bi100251u

Sequence

[bxe:Bxe_C1187]

Reference

3 [PMID:21425833]

Authors

Baker P, Carere J, Seah SY

Title

Probing the molecular basis of substrate specificity, stereospecificity, and catalysis in the class II pyruvate aldolase, BphI.

Journal

Biochemistry 50:3559-69 (2011)
DOI:10.1021/bi101947g

Sequence

[bxe:Bxe_C1187]

Reference

4 [PMID:21838275]

Authors

Carere J, Baker P, Seah SY

Title

Investigating the molecular determinants for substrate channeling in BphI-BphJ, an aldolase-dehydrogenase complex from the polychlorinated biphenyls degradation pathway.

Journal

Biochemistry 50:8407-16 (2011)
DOI:10.1021/bi200960j

Sequence

[bxe:Bxe_C1187]

Reference

5 [PMID:22081904]

Authors

Baker P, Seah SY

Title

Rational design of stereoselectivity in the class II pyruvate aldolase BphI.

Journal

J Am Chem Soc 134:507-13 (2012)
DOI:10.1021/ja208754r