KEGG ENZYME: 4.2.1.172

ENZYME: 4.2.1.172

Entry

EC 4.2.1.172                Enzyme

Name

trans-4-hydroxy-L-proline dehydratase

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

trans-4-hydroxy-L-proline hydro-lyase

Reaction(IUBMB)

trans-4-hydroxy-L-proline = (S)-1-pyrroline-5-carboxylate + H2O [RN:R11819]

Reaction(KEGG)

R11819

Substrate

trans-4-hydroxy-L-proline [CPD:C01157]

Product

(S)-1-pyrroline-5-carboxylate [CPD:C03912];
H2O [CPD:C00001]

Comment

The enzyme has been characterized from the bacterium Peptoclostridium difficile. The active form contains a glycyl radical that is generated by a dedicated activating enzyme via chemistry involving S-adenosyl-L-methionine (SAM) and a [4Fe-4S] cluster.

History

EC 4.2.1.172 created 2017

Pathway

ec00330

Arginine and proline metabolism

ec01100

Metabolic pathways

Orthology

K23121

trans-4-hydroxy-L-proline dehydratase

Genes

HMR:	Hipma_0362

DAV:	DESACE_04125

GSU:

GSU2101

GSK:	KN400_2127

GEB:

GM18_2359

GBM:

Gbem_2499

GBN:	GEOBRER4_17200

GER:	KP004_08265

GSUB:

KP001_06890

GNT:	KP003_07795

GPL:	M1B72_12535

PSYF:

N1030_14415

DSB:	LN040_10005

DBA:

Dbac_2366

DESU:

NLA06_01160

DPS:	DP0616(pflD)

DLM:	DPPLL_00240

DAT:	HRM2_04250(pflD1) HRM2_49330(pflD2)

DOV:	DSCO28_52190

DWD:	DSCW_54540

DALK:

DSCA_46900

DLI:

dnl_30770

DEK:	DSLASN_43020

EGV:	EGCR1_16435

EAV:	EH197_03950

ERAF:

J9537_19250

VLU:	M2919_03875

CBO:

CBO3183

CBA:

CLB_3219

CBH:

CLC_3093

CBY:

CLM_3595

CBL:

CLK_2581

CBB:

CLD_1348

CBI:

CLJ_B3455

CBF:

CLI_3322

CBM:

CBF_3314

CBJ:	H04402_03270

CKL:

CKL_0323

CKR:

CKR_0275

CSQ:

CSCA_0866

CLD:	CLSPO_c32860(pflD2)

CACE:

CACET_c25680(pflD2)

CCK:	Ccar_09825

CTYK:

CTK_C05150

CFM:	BJL90_19455

CARG:

RSJ17_07625

CDRK:

B9W14_14245

CFER:

D4Z93_05160

CCAA:

KQH81_14215

HHW:	NCTC503_02347(csdB_2)

CPRF:

K7H06_19485

CRAS:

KVH43_07795

OCW:	OW730_15950

OVA:

OBV_40980

CAML:

H6X83_10710

DOR:	Desor_3978

DAI:	Desaci_1175

DMI:	Desmer_3087

DRM:

Dred_2965

DRU:	Desru_0509

DFG:	B0537_08335

FWA:	DCMF_25705

ACAE:

HYG86_12305

CTHM:

CFE_0574

PROM:

QO263_00140

BPRM:

CL3_28280

CCOM:

I6K69_04740

BYL:	A4V09_06310

BPRO:

PMF13cell1_04098(cutC_3)

BLAR:

LC508_03850

BCOC:

BLCOC_31170(pflD)

CSCI:

HDCHBGLK_00253(cutC_1)

CHYL:

CE91St63_26530(pflD_2)

CSO:

CLS_03180

DLO:	K5I24_04245

SINT:

K5I26_05885

VGU:	HYG85_21430

MDV:	C5Q96_03640

MNL:	QU661_05190

AMT:

Amet_0584

GFE:	Gferi_16240

PBIF:

KXZ80_10205

PSOR:

RSJ16_12040

CDF:	CD630_32820(pflD)

PDC:	CDIF630_03582

CDC:	CD196_3097(pflD)

CDL:	CDR20291_3143(pflD)

PDF:	CD630DERM_32820(pflD)

PHX:	KGNDJEFE_00506(cutC_2)

TEM:	JW646_00595

TGC:	L0P85_07750

TMY:	TEMA_14950(pflD)

TEB:	T8CH_1917(pflD)

THYR:

P4S50_08750

TEP:	TepRe1_0044

TAE:	TepiRe1_0046

BACG:

D2962_11640

NTH:	Nther_2476

KME:	H0A61_00345(cutC)

SEDZ:

JYG23_11695

PUF:

UFO1_0399

PFT:

JBW_04226

MANA:

MAMMFC1_02761(bssA_3)

STED:

SPTER_40410(cutC_6)

AARG:

Aargi30884_27260(pflD_2)

ABSI:

A9CBEGH2_00460(pflD_1)

ERB:	A4V01_16645

CIU:	G4D55_13890

BSUE:

BS3272_00540

AMUC:

Pan181_08370(bssA)

PBU:	L21SP3_00214(csdB)

PBP:	STSP1_00751(csdB)

ALUS:

STSP2_03489(bssA)

GEP:	Q9293_04450

MSIL:

METEAL_32670

MSEA:

METESE_10340

CSOM:

MKD34_09420

TAI:

Taci_1437

CPOR:

BED41_02450

TLI:

Tlie_1574

AMO:	Anamo_1952

ARAM:

KAR29_12355

BVU:

BVU_1421

BDO:	EL88_01945

BDH:	GV66_04255

PSAC:

PSM36_1174

PDI:

BDI_0786

PARC:

CI960_07850

PMER:

INE87_00975(pflD)

PGOL:

K6V26_10100

PJO:	LK449_08315

PFAI:

NXY11_09110

OSP:	Odosp_3648

PCOU:

L6468_02095

TTZ:	FHG85_12835

BLQ:	L21SP5_00958(bssA)

DORI:

FH5T_08275

DRC:	G0Q07_01065

MCOS:

GM418_11240

ANF:

AQPE_2112

MBAS:

ALGA_0749

ATK:	AUTU_04410

OCI:	FEZ18_06875

SHYR:

LA303_00540 LA303_03965

TLE:

Tlet_1141

OCS:	OF820_05755

CPO:	COPRO5265_1565

DDF:	DEFDS_2103

DAP:	Dacet_1516

GTL:	EP073_08865

TKO:

TK0289

TSI:

TSIB_0631

TLT:

OCC_00382

ABI:

Aboo_0169

ACF:	AciM339_0879

KCR:

Kcr_0517

LOB:	NEF87_000394 NEF87_002435

PSYT:

DSAG12_03102

» show all

Reference

1 [PMID:28183913]

Authors

Levin BJ, Huang YY, Peck SC, Wei Y, Martinez-Del Campo A, Marks JA, Franzosa EA, Huttenhower C, Balskus EP

Title

A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans-4-hydroxy-l-proline.

Journal

Science 355:eaai8386 (2017)
DOI:10.1126/science.aai8386

Other DBs

ExplorEnz - The Enzyme Database:

4.2.1.172

IUBMB Enzyme Nomenclature:

4.2.1.172

ExPASy - ENZYME nomenclature database:

4.2.1.172

BRENDA, the Enzyme Database:

4.2.1.172

DBGET integrated database retrieval system