KEGG ENZYME: 4.2.2.25

ENZYME: 4.2.2.25

Entry

EC 4.2.2.25                 Enzyme

Name

gellan lyase

Class

Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides

Sysname

gellan beta-D-glucopyranosyl-(1->4)-D-glucopyranosyluronate lyase

Reaction(IUBMB)

Eliminative cleavage of beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyluronate bonds of gellan backbone releasing tetrasaccharides containing a 4-deoxy-4,5-unsaturated D-glucopyranosyluronic acid at the non-reducing end. The tetrasaccharide produced from deacetylated gellan is beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp.

Comment

The enzyme is highly specific to gellan, especially deacetylated gellan.

History

EC 4.2.2.25 created 2011

Orthology

K20610

gellan lyase

Reference

1 [PMID:9056228]

Authors

Hashimoto W, Maesaka K, Sato N, Kimura S, Yamamoto K, Kumagai H, Murata K

Title

Microbial system for polysaccharide depolymerization: enzymatic route for gellan depolymerization by Bacillus sp. GL1.

Journal

Arch Biochem Biophys 339:17-23 (1997)
DOI:10.1006/abbi.1996.9851

Sequence

[ag:BAA29068]

Reference

2 [PMID:9633595]

Authors

Hashimoto W, Sato N, Kimura S, Murata K

Title

Polysaccharide lyase: molecular cloning of gellan lyase gene and formation of the lyase from a huge precursor protein in Bacillus sp. GL1.

Journal

Arch Biochem Biophys 354:31-9 (1998)
DOI:10.1006/abbi.1998.0674

Sequence

[ag:BAA29068]

Reference

3 [PMID:14759609]

Authors

Miyake O, Kobayashi E, Nankai H, Hashimoto W, Mikami B, Murata K

Title

Posttranslational processing of polysaccharide lyase: maturation route for gellan lyase in Bacillus sp. GL1.

Journal

Arch Biochem Biophys 422:211-20 (2004)
DOI:10.1016/j.abb.2003.12.015

Sequence

[ag:BAA29068]

Other DBs

ExplorEnz - The Enzyme Database:

4.2.2.25

IUBMB Enzyme Nomenclature:

4.2.2.25

ExPASy - ENZYME nomenclature database:

4.2.2.25

BRENDA, the Enzyme Database:

4.2.2.25

All links

Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (43)   
   UniProt (41)   
   SWISS-PROT (2)   
DNA sequence (27)   
   GenBank (13)   
   EMBL (14)   
Protein domain (7)   
   InterPro (7)   
All databases (79)   

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