KEGG ENZYME: 4.2.3.114

ENZYME: 4.2.3.114

Entry

EC 4.2.3.114                Enzyme

Name

gamma-terpinene synthase;
OvTPS2;
ClcTS

Class

Lyases;
Carbon-oxygen lyases;
Acting on phosphates

Sysname

geranyl-diphosphate diphosphate-lyase (cyclizing, gamma-terpinene-forming)

Reaction(IUBMB)

geranyl diphosphate = gamma-terpinene + diphosphate [RN:R09969]

Reaction(KEGG)

R09969

Substrate

geranyl diphosphate [CPD:C00341]

Product

gamma-terpinene [CPD:C09900];
diphosphate [CPD:C00013]

Comment

Isolated from Thymus vulgaris (thyme) [1,2], Citrus limon (lemon) [3], Citrus unshiu (satsuma) [4] and Origanum vulgare (oregano) [5]. Requires Mg2+. Mn2+ less effective. The reaction involves a 1,2-hydride shift. The 5-pro-S hydrogen of geranyl diphosphate is lost. Traces of several other monoterpenoids are formed in addition to gamma-terpinene.

History

EC 4.2.3.114 created 2012

Orthology

K21931

gamma-terpinene synthase

K21939

(+)-sabinene/gamma-terpinene synthase

Genes

CIT:

102620613

Reference

1 [PMID:1897973]

Authors

Alonso WR, Croteau R

Title

Purification and characterization of the monoterpene cyclase gamma-terpinene synthase from Thymus vulgaris.

Journal

Arch Biochem Biophys 286:511-7 (1991)
DOI:10.1016/0003-9861(91)90073-R

Reference

2 [PMID:8460944]

Authors

LaFever RE, Croteau R

Title

Hydride shifts in the biosynthesis of the p-menthane monoterpenes alpha-terpinene, gamma-terpinene, and beta-phellandrene.

Journal

Arch Biochem Biophys 301:361-6 (1993)
DOI:10.1006/abbi.1993.1156

Reference

3 [PMID:12084056]

Authors

Lucker J, El Tamer MK, Schwab W, Verstappen FW, van der Plas LH, Bouwmeester HJ, Verhoeven HA

Title

Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases.

Journal

Eur J Biochem 269:3160-71 (2002)
DOI:10.1046/j.1432-1033.2002.02985.x

Sequence

[ag:AAM53943]

Reference

4 [PMID:15630174]

Authors

Suzuki Y, Sakai H, Shimada T, Omura M, Kumazawa S, Nakayama T

Title

Characterization of gamma-terpinene synthase from Citrus unshiu (Satsuma mandarin).

Journal

Biofactors 21:79-82 (2004)
DOI:10.1002/biof.552210115

Reference

5 [PMID:20419468]

Authors

Crocoll C, Asbach J, Novak J, Gershenzon J, Degenhardt J

Title

Terpene synthases of oregano (Origanum vulgare L.) and their roles in the pathway and regulation of terpene biosynthesis.

Journal

Plant Mol Biol 73:587-603 (2010)
DOI:10.1007/s11103-010-9636-1

Other DBs

ExplorEnz - The Enzyme Database:

4.2.3.114

IUBMB Enzyme Nomenclature:

4.2.3.114

ExPASy - ENZYME nomenclature database:

4.2.3.114

BRENDA, the Enzyme Database:

4.2.3.114

DBGET integrated database retrieval system