KEGG   ENZYME: 4.2.3.128
Entry
EC 4.2.3.128                Enzyme                                 
Name
beta-cubebene synthase;
cop4;
Mg25
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, beta-cubebene-forming)
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate = beta-cubebene + diphosphate [RN:R10007]
Reaction(KEGG)
R10007
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448]
Product
beta-cubebene [CPD:C09648];
diphosphate [CPD:C00013]
Comment
Isolated from the fungus Coprinus cinereus. The enzyme also forms (+)-delta-cadinene, beta-copaene, (+)-sativene and traces of several other sequiterpenoids [2-4]. It is found in many higher plants such as Magnolia grandiflora (Southern Magnolia) together with germacrene A [1]. See EC 4.2.3.13, (+)-delta-cadinene synthase, EC 4.2.3.127, beta-copaene synthase, EC 4.2.3.129, (+)-sativene synthase, and EC 4.2.3.23, germacrene A synthase.
History
EC 4.2.3.128 created 2012
Orthology
K21982  beta-cubebene synthase
K22058  cubebol/beta-copaene/beta-cubebene/(+)-sativene synthase
Genes
MSIN131221682 131221684 131221691
CCICC1G_06441
Reference
1  [PMID:18467455]
  Authors
Lee S, Chappell J
  Title
Biochemical and genomic characterization of terpene synthases in Magnolia grandiflora.
  Journal
Plant Physiol 147:1017-33 (2008)
DOI:10.1104/pp.108.115824
  Sequence
Reference
2  [PMID:19400802]
  Authors
Agger S, Lopez-Gallego F, Schmidt-Dannert C
  Title
Diversity of sesquiterpene synthases in the basidiomycete Coprinus cinereus.
  Journal
Mol Microbiol 72:1181-95 (2009)
DOI:10.1111/j.1365-2958.2009.06717.x
  Sequence
Reference
3  [PMID:20419721]
  Authors
Lopez-Gallego F, Agger SA, Abate-Pella D, Distefano MD, Schmidt-Dannert C
  Title
Sesquiterpene synthases Cop4 and Cop6 from Coprinus cinereus: catalytic promiscuity and cyclization of farnesyl pyrophosphate geometric isomers.
  Journal
Chembiochem 11:1093-106 (2010)
DOI:10.1002/cbic.200900671
  Sequence
Reference
4  [PMID:20889795]
  Authors
Lopez-Gallego F, Wawrzyn GT, Schmidt-Dannert C
  Title
Selectivity of fungal sesquiterpene synthases: role of the active site's H-1 alpha loop in catalysis.
  Journal
Appl Environ Microbiol 76:7723-33 (2010)
DOI:10.1128/AEM.01811-10
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.128
IUBMB Enzyme Nomenclature: 4.2.3.128
ExPASy - ENZYME nomenclature database: 4.2.3.128
BRENDA, the Enzyme Database: 4.2.3.128

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