KEGG   ENZYME: 4.2.3.132
Entry
EC 4.2.3.132                Enzyme                                 
Name
neoabietadiene synthase;
TPS-LAS
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
(+)-copaly-diphosphate diphosphate-lyase (cyclizing, neoabietadiene-forming)
Reaction(IUBMB)
(+)-copalyl diphosphate = neoabietadiene + diphosphate [RN:R06303]
Reaction(KEGG)
R06303
Substrate
(+)-copalyl diphosphate [CPD:C11901]
Product
neoabietadiene [CPD:C11880];
diphosphate [CPD:C00013]
Comment
Isolated from Abies grandis (grand fir) [1]. This class I enzyme forms about equal proportions of abietadiene, levopimaradiene and neoabietadiene. See also EC 4.2.3.18, abieta-7,13-diene synthase and EC 4.2.3.32, levopimaradiene synthase. An X-ray study of this multifunctional enzyme showed that the class I activity is in the alpha domain, while (+)-copalyl diphosphate synthase activity (EC 5.5.1.12, a class II activity) is in the beta and gamma domains [2]. In Pinus taeda (loblolly pine) the major product is levopimaradiene, with less abietadiene and neoabietadiene [3].
History
EC 4.2.3.132 created 2012
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K12927  abietadiene/neoabietadiene/copalyl diphosphate synthase
K19571  levopimaradiene/neoabietadiene/copalyl diphosphate synthase
Reference
1  [PMID:11112547]
  Authors
Peters RJ, Flory JE, Jetter R, Ravn MM, Lee HJ, Coates RM, Croteau RB.
  Title
Abietadiene synthase from grand fir (Abies grandis): characterization and mechanism of action of the "pseudomature" recombinant enzyme.
  Journal
Biochemistry 39:15592-602 (2000)
DOI:10.1021/bi001997l
  Sequence
[ag:AAB05407]
Reference
2  [PMID:22219188]
  Authors
Zhou K, Gao Y, Hoy JA, Mann FM, Honzatko RB, Peters RJ
  Title
Insights into diterpene cyclization from structure of bifunctional abietadiene synthase from Abies grandis.
  Journal
J Biol Chem 287:6840-50 (2012)
DOI:10.1074/jbc.M111.337592
  Sequence
[ag:AAB05407]
Reference
3  [PMID:16497345]
  Authors
Ro DK, Bohlmann J
  Title
Diterpene resin acid biosynthesis in loblolly pine (Pinus taeda): functional characterization of abietadiene/levopimaradiene synthase (PtTPS-LAS) cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase (PtAO, CYP720B1).
  Journal
Phytochemistry 67:1572-8 (2006)
DOI:10.1016/j.phytochem.2006.01.011
  Sequence
[ag:AAX07435]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.132
IUBMB Enzyme Nomenclature: 4.2.3.132
ExPASy - ENZYME nomenclature database: 4.2.3.132
BRENDA, the Enzyme Database: 4.2.3.132

  All links  
Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (3)   
   KEGG COMPOUND (3)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (4)   
   KEGG ORTHOLOGY (2)   
   KEGG GENES (2)   
Protein sequence (12)   
   UniProt (6)   
   SWISS-PROT (6)   
Protein domain (8)   
   InterPro (8)   
All databases (33)   

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