KEGG   ENZYME: 4.2.3.149
Entry
EC 4.2.3.149                Enzyme                                 
Name
nephthenol synthase;
DtcycA (gene name);
DtcycB (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
geranylgeranyl-diphosphate diphosphate-lyase [(R)-nephthenol-forming]
Reaction(IUBMB)
geranylgeranyl diphosphate + H2O = (R)-nephthenol + diphosphate [RN:R10897]
Reaction(KEGG)
R10897
Substrate
geranylgeranyl diphosphate [CPD:C00353];
H2O [CPD:C00001]
Product
(R)-nephthenol [CPD:C20923];
diphosphate [CPD:C00013]
Comment
Requires Mg2+. Two isozymes with this activity were isolated from the bacterium Streptomyces sp. SANK 60404. The enzyme encoded by the DtcycA gene also produces cembrene C (see EC 4.2.3.148, cembrene C synthase), while the enzyme encoded by the DtcycB gene also produces (R)-cembrene A and (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol (see EC 4.2.3.150, cembrene A synthase, and EC 4.2.3.151, pentamethylcyclopentadecatrienol synthase).
History
EC 4.2.3.149 created 2014
Orthology
K20504  cembrene C/nephthenol synthase
K20505  nephthenol/cembrene A/pentamethylcyclopentadecatrienol synthase
Reference
1  [PMID:23386483]
  Authors
Meguro A, Tomita T, Nishiyama M, Kuzuyama T
  Title
Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton.
  Journal
Chembiochem 14:316-21 (2013)
DOI:10.1002/cbic.201200651
  Sequence
[ag:BAM78697 BAM78698]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.149
IUBMB Enzyme Nomenclature: 4.2.3.149
ExPASy - ENZYME nomenclature database: 4.2.3.149
BRENDA, the Enzyme Database: 4.2.3.149

  All links  
Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (4)   
   KEGG ORTHOLOGY (2)   
   KEGG GENES (2)   
Protein sequence (4)   
   UniProt (2)   
   SWISS-PROT (2)   
Protein domain (3)   
   InterPro (3)   
All databases (17)   

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