KEGG ENZYME: 4.2.3.154

ENZYME: 4.2.3.154

Entry

EC 4.2.3.154                Enzyme

Name

demethyl-4-deoxygadusol synthase;
Nos2 (gene name);
Anb2 (gene name)

Class

Lyases;
Carbon-oxygen lyases;
Acting on phosphates

Sysname

D-sedoheptulose-7-phosphate phosphate-lyase (cyclizing; demethyl-4-deoxygadusol-forming)

Reaction(IUBMB)

D-sedoheptulose 7-phosphate = demethyl-4-deoxygadusol + phosphate + H2O [RN:R11326]

Reaction(KEGG)

R11326

Substrate

D-sedoheptulose 7-phosphate [CPD:C05382]

Product

demethyl-4-deoxygadusol [CPD:C21281];
phosphate [CPD:C00009];
H2O [CPD:C00001]

Comment

The enzyme, characterized from the cyanobacterium Nostoc punctiforme PCC 73102, is involved in the biosynthesis of the sunscreen compound shinorine. It requires a divalent metal ion (Zn2+ or Co2+) and an NAD+ cofactor, which is transiently reduced during the reaction. cf. EC 4.2.3.152, 2-epi-5-epi-valiolone synthase and EC 4.2.3.155, 2-epi-valiolone synthase.

History

EC 4.2.3.154 created 2016

Orthology

K21342

demethyl-4-deoxygadusol synthase

Genes

MPAG:

C0J29_21490

MCOO:

MCOO_09320(aroB_2)

MGAU:

MGALJ_52870

MPAA:

MKK62_12420

MWU:	PT015_05885

MCB:	Mycch_0151

MAUU:

NCTC10437_02576(aroB_2)

MMAG:

MMAD_33660(aroB_2)

MTY:	MTOK_57150(aroB_2)

MPSC:

MPSYJ_07130(aroB_1)

MARZ:

MARA_24090(aroB_2)

MSEI:

MSEDJ_27540(aroB_1)

MRF:	MJO55_00790

MGRO:

FZ046_13290

RHB:

NY08_2748

RFA:	A3L23_00895(aroB_1)

RHS:	A3Q41_02456(aroB_1)

RHU:	A3Q40_02457(aroB_2)

RANT:

RHODO2019_15770

RHOP:

D8W71_12170

WHR:	OG579_03875

ALX:	LVQ62_07140

NOO:	FE634_11545

NAQU:

ENKNEFLB_00668

AER:	AERYTH_01590

NBT:	KLP28_04910

NAKA:

H7F38_19490

PSEA:

WY02_06440

PPEL:

H6H00_24785

APRE:

CNX65_21345

AMI:

Amir_4259

ACTY:

OG774_14535

IMA:	PO878_16450

CMP:	Cha6605_2820

CEP:	Cri9333_2379

LEN:	LEP3755_23080

TSIN:

OXH18_18240

CHON:

NIES4102_34930

MPK:

VL20_4964

CYC:	PCC7424_4981

ONI:	Osc7112_3782

NPU:	Npun_R5600

NOP:	Nos7524_3370

NON:	NOS3756_20030

NFL:	COO91_06028

NOE:	CLI64_23900

NSH:

GXM_06694

NED:	HUN01_27795

AVA:

Ava_3858

ANN:	EH233_17480

CSG:	Cylst_1339

HBQ:	QI031_21975

CALT:

Cal6303_3580

CALS:

NIES3974_03010

RIV:	Riv7116_0138

NSPH:

BDGGKGIB_03892

NCN:	BZZ01_16735

CTHE:

Chro_0778

SCS:	Sta7437_1639

STAN:

STA3757_07800

» show all

Reference

1 [PMID:20813918]

Authors

Balskus EP, Walsh CT

Title

The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria.

Journal

Science 329:1653-6 (2010)
DOI:10.1126/science.1193637

Sequence

[npu:Npun_R5600] [ava:Ava_3858]

Reference

2 [PMID:22741921]

Authors

Asamizu S, Xie P, Brumsted CJ, Flatt PM, Mahmud T

Title

Evolutionary divergence of sedoheptulose 7-phosphate cyclases leads to several distinct cyclic products.

Journal

J Am Chem Soc 134:12219-29 (2012)
DOI:10.1021/ja3041866

Sequence

[npu:Npun_R5600] [ava:Ava_3858]

Other DBs

ExplorEnz - The Enzyme Database:

4.2.3.154

IUBMB Enzyme Nomenclature:

4.2.3.154

ExPASy - ENZYME nomenclature database:

4.2.3.154

BRENDA, the Enzyme Database:

4.2.3.154

DBGET integrated database retrieval system