KEGG   ENZYME: 4.2.3.27
Entry
EC 4.2.3.27                 Enzyme                                 
Name
isoprene synthase;
ISPC;
ISPS;
dimethylallyl-diphosphate diphosphate-lyase (isoprene-forming)
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
prenyl-diphosphate diphosphate-lyase (isoprene-forming)
Reaction(IUBMB)
prenyl diphosphate = isoprene + diphosphate [RN:R08199]
Reaction(KEGG)
R08199
Substrate
prenyl diphosphate [CPD:C00235]
Product
isoprene [CPD:C16521];
diphosphate [CPD:C00013]
Comment
Requires Mg2+ or Mn2+ for activity. This enzyme is located in the chloroplast of isoprene-emitting plants, such as poplar and aspen, and may be activitated by light-dependent changes in chloroplast pH and Mg2+ concentration [2,8].
History
EC 4.2.3.27 created 2007
Pathway
ec00900  Terpenoid backbone biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K12742  isoprene synthase
Genes
ALY9306207
CRB17880730
CSAT104718686 104723429 104731915
BRP103869246
BNA106437444
BOE106301302
RSZ108835531
THJ104809623 104813173 104814037 104817852
CIT102626262
PVY116123255 116128463 116137959
MINC123201668 123222461 123222493 123223710 123223711 123223712
GRA105780734 105780735
GHI107898356 107948996
GAB108459891
HSYR120137220 120142667 120150919 120150921
DZI111281612 111282169
EGR104424788 104424789 104424822 104427170 104427201 104427202 104427205 104430709 120289311 120289653 120290023
GMX100782452(TPS3) 100786684(TPS23) 100801629(TPS8) 100814880(TPS9)
GSJ114367474 114378664 114402973 114417283
PVUPHAVU_005G133100g PHAVU_005G133200g PHAVU_005G133300g PHAVU_005G135000g PHAVU_005G135100g PHAVU_005G135200g PHAVU_011G1068000 PHAVU_011G107100g PHAVU_011G107200g
VRA106758286 106758670 106758896 106771573 106780025 106780479
VAR108332923 108339360 108339363
VUN114168156 114168157 114170371 114184762 114184763 114190704
VUM124822890 124831682 124833701
CCAJ109788984 109793359 109795221 109800348 109812765 109817029 109817034
APRC113849434 113859711 113859712 113870923
TPRA123881736
PSAT127104156 127107065 127107066
LJALj0g3v0356449.1(Lj0g3v0356449.1) Lj3g3v2888440.1(Lj3g3v2888440.1) Lj3g3v2888450.1(Lj3g3v2888450.1)
ADU107461928 107462094 107467723 107472126 107477816 127744051
AIP107614308 107614309 107619403 107629942
AHF112707039 112707040 112712397 112737563 112772760 112772761 112779715 112789658
LANG109355202
PCIN129297098 129312385 129313230
QSAO6P43_000625 O6P43_014061 O6P43_028726
FVE101297281 101305692 101310344
RCN112170738 112187983 112191500
MDM103404781
MSYL126611736 126612183
PXB103946958 103946959
ZJU107409508 107416905 107418472
MNT21401277 21401279
RCU8260996
JCU105631119 105631122 105644031 105648248
HBR110634890 110651136
MESC110627516 110627517 110627952
POP18100875 18107022 7485020
PEU105110039 105112403 105112405 105119775
PALZ118040300 118040301 118054959 118054961
JRE108979116 108991961 108991983
CILL122303857 122303892
CAVE132181657 132181663 132182366 132182685 132182717 132182729 132182934
QSU111989249 111989265 112037150
QLO115955012 115955086 115956777 115957700 115981122
TWL119992103 120007178
VVI100243167 100263737 100265475
VRI117926341 117926354 117926476
INI109163391 109163393 109163396 109163416 109188031
ITR116010101 116010102 116032902 116033532
SIND105165226
OEU111368363 111374106 111383800 111408326
SSPL121773664 121773702 121773788 121773820 121773925
APAN127247760
HAN110874622 110876334 110878487 110878488
ECAD122586432 122587667 122595421 122600593
LSV111881302 111916241 111917018 111917020
CCAV112529037
DCR108192517 108224147
CSIN114259935 114281498 114295355
RVL131323085 131323086 131323087 131323088 131323090 131323628 131323633
AEW130791847
BVG104883790 104890251
SOE110781021 110787756
CQI110698900 110703332
ATRI130810958 130817596
MOF131148670
PSOM113320214 113323354 113325331 113344198 113344201 113358522
BDI100839060 100839782 104583049
ATS109735704 109764078 109777110
TDC119333939 119358627 119360217 119360221 119366824 119366829
SBI8081467 8083449
PVIR120691740
PDA103698991 103699093 103699243 103704724
EGU105046866 105057539
DCT110097122 110100458 110100459
PEQ110032081
AOF109828583 109830761 109833317 109833319
MSIN131218762 131220240 131221636 131229650 131237670 131237732 131237738 131256969
 » show all
Reference
1  [PMID:16668590]
  Authors
Silver GM, Fall R.
  Title
Enzymatic Synthesis of Isoprene from Dimethylallyl Diphosphate in Aspen Leaf Extracts.
  Journal
Plant Physiol 97:1588-1591 (1991)
DOI:10.1104/pp.97.4.1588
Reference
2  [PMID:7768893]
  Authors
Silver GM, Fall R.
  Title
Characterization of aspen isoprene synthase, an enzyme responsible for leaf isoprene emission to the atmosphere.
  Journal
J Biol Chem 270:13010-6 (1995)
DOI:10.1074/jbc.270.22.13010
Reference
3  [PMID:12226383]
  Authors
Wildermuth MC, Fall R.
  Title
Light-Dependent Isoprene Emission (Characterization of a Thylakoid-Bound Isoprene Synthase in Salix discolor Chloroplasts).
  Journal
Plant Physiol 112:171-182 (1996)
DOI:10.1104/pp.112.1.171
Reference
4
  Authors
Schnitzler, J.P., Arenz, R., Steinbrecher, R. and Lehming, A.
  Title
Characterization of an isoprene synthase from leaves of Quercus petraea.
  Journal
Bot Acta 109:216-221 (1996)
Reference
5  [PMID:11506373]
  Authors
Miller B, Oschinski C, Zimmer W
  Title
First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli.
  Journal
Planta 213:483-7 (2001)
DOI:10.1007/s004250100557
  Sequence
Reference
6  [PMID:12054742]
  Authors
Sivy TL, Shirk MC, Fall R.
  Title
Isoprene synthase activity parallels fluctuations of isoprene release during growth of Bacillus subtilis.
  Journal
Biochem Biophys Res Commun 294:71-5 (2002)
DOI:10.1016/S0006-291X(02)00435-7
Reference
7  [PMID:15848197]
  Authors
Sasaki K, Ohara K, Yazaki K
  Title
Gene expression and characterization of isoprene synthase from Populus alba.
  Journal
FEBS Lett 579:2514-8 (2005)
DOI:10.1016/j.febslet.2005.03.066
  Sequence
Reference
8  [PMID:16052321]
  Authors
Schnitzler JP, Zimmer I, Bachl A, Arend M, Fromm J, Fischbach RJ.
  Title
Biochemical properties of isoprene synthase in poplar (Populus x canescens).
  Journal
Planta 222:777-86 (2005)
DOI:10.1007/s00425-005-0022-1
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.27
IUBMB Enzyme Nomenclature: 4.2.3.27
ExPASy - ENZYME nomenclature database: 4.2.3.27
BRENDA, the Enzyme Database: 4.2.3.27
CAS: 139172-14-8

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