KEGG ENZYME: 4.2.3.86

ENZYME: 4.2.3.86

Entry

EC 4.2.3.86                 Enzyme

Name

7-epi-alpha-selinene synthase

Class

Lyases;
Carbon-oxygen lyases;
Acting on phosphates

Sysname

(2E,6E)-farnesyl-diphosphate diphosphate-lyase (7-epi-alpha-selinene-forming)

Reaction(IUBMB)

(2E,6E)-farnesyl diphosphate = 7-epi-alpha-selinene + diphosphate [RN:R09886]

Reaction(KEGG)

R09886

Substrate

(2E,6E)-farnesyl diphosphate [CPD:C00448]

Product

7-epi-alpha-selinene [CPD:C20159];
diphosphate [CPD:C00013]

Comment

The recombinant enzyme from Vitis vinifera forms 49.5% (+)-valencene (cf. EC 4.2.3.73, valencene synthase) and 35.5% (-)-7-epi-alpha-selinene. Initial cyclization gives (+)-germacrene A in an enzyme bound form which is not released to the medium.

History

EC 4.2.3.86 created 2011

Pathway

ec00909

Sesquiterpenoid and triterpenoid biosynthesis

Orthology

K14181

valencene/7-epi-alpha-selinene synthase

Genes

VVI:	100232955(VALCS) 100246608 100249970 100256880 100260112 100260210(GERA) 100263746 100267137 100267145

VRI:	117906216 117906289 117907590

ATRI:

130809483

Reference

1 [PMID:15464152]

Authors

Lucker J, Bowen P, Bohlmann J

Title

Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries.

Journal

Phytochemistry 65:2649-59 (2004)
DOI:10.1016/j.phytochem.2004.08.017

Sequence

[vvi:100232955]

Reference

2 [PMID:19359488]

Authors

Martin DM, Toub O, Chiang A, Lo BC, Ohse S, Lund ST, Bohlmann J

Title

The bouquet of grapevine (Vitis vinifera L. cv. Cabernet Sauvignon) flowers arises from the biosynthesis of sesquiterpene volatiles in pollen grains.

Journal

Proc Natl Acad Sci U S A 106:7245-50 (2009)
DOI:10.1073/pnas.0901387106