KEGG ENZYME: 4.2.3.96

ENZYME: 4.2.3.96

Entry

EC 4.2.3.96                 Enzyme

Name

avermitilol synthase

Class

Lyases;
Carbon-oxygen lyases;
Acting on phosphates

Sysname

avermitilol hydrolase (cyclizing, avermitilol-forming)

Reaction(IUBMB)

(2E,6E)-farnesyl diphosphate + H2O = avermitilol + diphosphate [RN:R09896]

Reaction(KEGG)

R09896

Substrate

(2E,6E)-farnesyl diphosphate [CPD:C00448];
H2O [CPD:C00001]

Product

avermitilol [CPD:C20181];
diphosphate [CPD:C00013]

Comment

Requires Mg2+. The recombinent enzyme gives avermitilol (85%) plus traces of germacrene A, germacrene B and viridiflorol. The (1S)-hydrogen of farnesyl diphosphate is retained.

History

EC 4.2.3.96 created 2012

Pathway

ec00909

Sesquiterpenoid and triterpenoid biosynthesis

Orthology

K18110

avermitilol synthase

Genes

SMA:	SAVERM_76(ams)

STRM:

M444_02785

SVN:	CP980_01435

SNF:	JYK04_00062(tpc1_1)

SMOB:

J7W19_05280

SXT:	KPP03845_100097(tpc1)

SGK:	PET44_01335

STRH:

GXP74_03530

Reference

1 [PMID:20536237]

Authors

Chou WK, Fanizza I, Uchiyama T, Komatsu M, Ikeda H, Cane DE

Title

Genome mining in Streptomyces avermitilis: cloning and characterization of SAV_76, the synthase for a new sesquiterpene, avermitilol.

Journal

J Am Chem Soc 132:8850-1 (2010)
DOI:10.1021/ja103087w

Sequence

[sma:SAVERM_76]

Other DBs

ExplorEnz - The Enzyme Database:

4.2.3.96

IUBMB Enzyme Nomenclature:

4.2.3.96

ExPASy - ENZYME nomenclature database:

4.2.3.96

BRENDA, the Enzyme Database:

4.2.3.96

DBGET integrated database retrieval system