KEGG ENZYME: 4.2.99.24

ENZYME: 4.2.99.24

Entry

EC 4.2.99.24                Enzyme

Name

thebaine synthase;
THS

Class

Lyases;
Carbon-oxygen lyases;
Other carbon-oxygen lyases

Sysname

salutaridinol 7-O-acetate acetate-lyase (thebaine-forming)

Reaction(IUBMB)

salutaridinol 7-O-acetate = thebaine + acetate [RN:R04769]

Reaction(KEGG)

R04769

Substrate

salutaridinol 7-O-acetate [CPD:C05322]

Product

thebaine [CPD:C06173];
acetate [CPD:C00033]

Comment

Isolated from the plant Papaver somniferum (opium poppy). The reaction occurs spontaneously when the pH is between 8-9, but the enzyme is required at the physiological pH, which is close to 7.

History

EC 4.2.99.24 created 2019

Pathway

ec00950

Isoquinoline alkaloid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K01742

thebaine synthase

Genes

PSOM:

113322169 113340172

Reference

1 [PMID:29807982]

Authors

Chen X, Hagel JM, Chang L, Tucker JE, Shiigi SA, Yelpaala Y, Chen HY, Estrada R, Colbeck J, Enquist-Newman M, Ibanez AB, Cottarel G, Vidanes GM, Facchini PJ

Title

A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis.

Journal

Nat Chem Biol 14:738-743 (2018)
DOI:10.1038/s41589-018-0059-7

Sequence

[psom:113322169]

Other DBs

ExplorEnz - The Enzyme Database:

4.2.99.24

IUBMB Enzyme Nomenclature:

4.2.99.24

ExPASy - ENZYME nomenclature database:

4.2.99.24

BRENDA, the Enzyme Database:

4.2.99.24

DBGET integrated database retrieval system