KEGG ENZYME: 4.3.2.5

ENZYME: 4.3.2.5

Entry

EC 4.3.2.5                  Enzyme

Name

peptidylamidoglycolate lyase;
alpha-hydroxyglycine amidating dealkylase;
peptidyl-alpha-hydroxyglycine alpha-amidating lyase;
HGAD;
PGL;
PAL;
peptidylamidoglycolate peptidylamide-lyase

Class

Lyases;
Carbon-nitrogen lyases;
Amidine-lyases

Sysname

[peptide]-(2S)-2-hydroxyglycine peptidyl-amide-lyase (glyoxylate-forming)

Reaction(IUBMB)

[peptide]-(2S)-2-hydroxyglycine = [peptide]-amide + glyoxylate [RN:R03874]

Reaction(KEGG)

R03874

Substrate

[peptide]-(2S)-2-hydroxyglycine [CPD:C03303]

Product

[peptide]-amide [CPD:C02179];
glyoxylate [CPD:C00048]

Comment

Requires zinc. The enzyme acts on the product of the reaction catalysed by EC 1.14.17.3 peptidylglycine monooxygenase, thus removing a terminal glycine residue and leaving a des-glycine peptide amide. In mammals, the two activities are part of a bifunctional protein.

History

EC 4.3.2.5 created 1992, modified 2019

Orthology

K18200

peptidylamidoglycolate lyase

K24006

peptidylglycine monooxygenase / peptidylamidoglycolate lyase

Genes

HSA:

5066(PAM)

PTR:	461974(PAM)

PPS:	100970635(PAM)

GGO:	101148887(PAM)

PON:	100449730(PAM)

NLE:	100603954(PAM)

HMH:	116470968(PAM)

MCC:	707733(PAM)

MCF:	101926659(PAM)

MTHB:

126957228

MNI:	105471068(PAM)

CSAB:

103244283(PAM)

CATY:

105576668(PAM)

PANU:

101026672(PAM)

TGE:	112626915(PAM)

MLEU:

105554887(PAM)

RRO:	104669199(PAM)

RBB:	108527716(PAM)

TFN:	117064857(PAM)

PTEH:

111542665(PAM)

CANG:

105524723(PAM)

CJC:	100396001(PAM)

SBQ:	101034151(PAM)

CIMI:

108291406(PAM)

CSYR:

103263009(PAM)

MMUR:

105875983(PAM)

LCAT:

123648435(PAM)

PCOQ:

105807623(PAM)

OGA:	100956802(PAM)

MMU:	18484(Pam)

MCAL:

110305227(Pam)

MPAH:

110321660(Pam)

RNO:	25508(Pam)

MCOC:

116088825(Pam)

ANU:	117722233(Pam)

MUN:	110540758(Pam)

CGE:	100770162(Pam)

MAUA:

101829991(Pam)

PROB:

127226743(Pam)

PLEU:

114699806(Pam)

MORG:

121433406(Pam)

MFOT:

126508296

AAMP:

119820391(Pam)

NGI:	103738185(Pam)

HGL:	101716909(Pam)

CPOC:

100713254(Pam)

CCAN:

109689129(Pam) 109698017

DORD:

105989825(Pam)

DSP:	122117119(Pam)

PLOP:

125339850(Pam)

NCAR:

124986502

MMMA:

107140778(Pam)

OCU:

100352005

OPI:	101526566(PAM)

TUP:	102499925(PAM)

GVR:	103593467(PAM)

CFA:	479145(PAM)

CLUD:

112654112(PAM)

VVP:	112915565(PAM)

VLG:	121489468(PAM)

NPO:	129501613(PAM)

AML:	100484534(PAM)

UMR:	103656467(PAM)

UAH:	113255028(PAM)

UAR:	123799268(PAM)

ELK:

111148403

LLV:

125101073

MPUF:

101671790(PAM)

MNP:	132028823(PAM)

MLK:	131832499(PAM)

NVS:	122917187(PAM)

ORO:	101383156(PAM)

EJU:	114208638(PAM)

ZCA:	113929120(PAM)

MLX:	118017056(PAM)

NSU:	110590755(PAM)

LWW:	102745099(PAM)

FCA:	101084598(PAM)

PYU:	121012392(PAM)

PCOO:

112860269(PAM)

PBG:	122491636(PAM)

PVIV:

125161563(PAM)

LRUF:

124519496

PTG:	102961161(PAM)

PPAD:

109267636(PAM)

PUC:

125934337

AJU:

106982276

HHV:	120220005(PAM)

BTA:	280890(PAM)

BOM:	102271872(PAM)

BIU:	109562372(PAM)

BBUB:

102409727(PAM)

BBIS:

104982553(PAM)

CHX:	102190130(PAM)

OAS:	101112815(PAM)

BTAX:

128051451(PAM)

ODA:	120863478(PAM)

CCAD:

122439492(PAM)

MBEZ:

129558554(PAM)

SSC:	100526040(PAM)

CFR:	102509591(PAM)

CBAI:

105082012(PAM)

CDK:	105103083(PAM)

VPC:	102529765(PAM)

BACU:

103008694(PAM)

BMUS:

118893484(PAM)

LVE:	103088789(PAM)

OOR:	101274063(PAM)

DLE:	111183960(PAM)

PCAD:

102993897(PAM)

PSIU:

116750498(PAM)

NASI:

112396130(PAM)

ECB:	791244(PAM)

EPZ:	103555847(PAM)

EAI:	106831159(PAM)

MYB:	102257709(PAM)

MYD:	102752613(PAM)

MMYO:

118655718(PAM)

MLF:	102422620(PAM)

PKL:	118703757(PAM)

EFUS:

103288742(PAM)

MNA:	107544178(PAM)

DRO:	112307140(PAM)

SHON:

118977247(PAM)

AJM:	119047249(PAM)

PDIC:

114494537(PAM)

PHAS:

123818032(PAM)

MMF:	118641919(PAM)

PPAM:

129083931(PAM)

HAI:	109394174(PAM)

RFQ:	117024680(PAM)

PALE:

102894575(PAM)

PGIG:

120590776(PAM)

PVP:	105304427(PAM)

RAY:	107509145(PAM)

MJV:	108391703(PAM)

TOD:	119260954(PAM)

SARA:

101547265(PAM)

LAV:	100655604(PAM)

TMU:

101356870

ETF:	101662573(PAM)

DNM:	101428897(PAM)

MDO:	100030601(PAM)

GAS:	123236274(PAM)

SHR:	100929728(PAM)

AFZ:

127560527

PCW:	110199219(PAM)

OAA:	100073355(PAM)

GGA:	427274(PAM)

PCOC:

116229802(PAM)

CJO:	107306340(PAM)

TPAI:

128089489(PAM)

LMUT:

125687282(PAM)

NMEL:

110389678(PAM)

APLA:

101795983(PAM)

ACYG:

106043740(PAM)

CATA:

118260084(PAM)

AFUL:

116500419(PAM)

TGU:	100227923(PAM)

LSR:	110477851(PAM)

SCAN:

103822855(PAM)

PMOA:

120500582(PAM)

OTC:	121332561(PAM)

PRUF:

121355586(PAM)

GFR:	102039677(PAM)

FAB:	101821831(PAM)

OMA:	130264919(PAM)

PHI:	102114573(PAM)

PMAJ:

107215969(PAM)

CCAE:

111940620(PAM)

CCW:	104693194(PAM)

CBRC:

103613059(PAM)

ETL:	114064047(PAM)

ZAB:	102069666(PAM)

SVG:	106863061(PAM)

MMEA:

130586850

HRT:	120765045(PAM)

FPG:	101920860(PAM)

FCH:	102052743(PAM)

CLV:	102087217(PAM)

EGZ:	104135057(PAM)

NNI:	104010298(PAM)

PCRI:

104029410(PAM)

PLET:

104619390(PAM)

EHS:	104507079(PAM)

PCAO:

104044911(PAM)

ACUN:

113489698(PAM)

TALA:

104363524(PAM)

PADL:

103920863(PAM)

AFOR:

103903061(PAM)

ACHC:

115336613(PAM)

HALD:

104316339(PAM)

HLE:	104830258(PAM)

AGEN:

126036477

GCL:

127027149

CCRI:

104158631(PAM)

CSTI:

104549454

CMAC:

104484951(PAM)

BREG:

104629990(PAM)

FGA:	104074433(PAM)

LDI:	104346787(PAM)

OHA:	104327527(PAM)

NNT:	104402404(PAM)

SHAB:

115619349(PAM)

DPUB:

104302573(PAM)

PGUU:

104464770(PAM)

ACAR:

104522283(PAM)

CPEA:

104397259(PAM)

AVIT:

104279353(PAM)

CVF:	104292311(PAM)

RTD:	128902291(PAM)

CUCA:

104058390(PAM)

TEO:	104374810(PAM)

AROW:

112970164(PAM)

NPD:	112951384(PAM)

TGT:	104568553(PAM)

DNE:	112988498 112992418

SCAM:

104140158(PAM)

ASN:	102374229(PAM)

AMJ:	102559599(PAM)

CPOO:

109308144(PAM)

GGN:	109297455(PAM)

PSS:	102462977(PAM)

CMY:	102946187(PAM)

CCAY:

125636433(PAM)

DCC:	119855451(PAM)

CPIC:

101942761(PAM)

TST:	117879396(PAM)

CABI:

116815270(PAM)

MRV:	120407700(PAM)

ACS:	100562563(pam)

ASAO:

132767177(PAM)

PVT:	110079386(PAM)

SUND:

121924102(PAM)

PBI:	103055156(PAM)

CTIG:

120306039(PAM)

TSR:	106548589(PAM)

PGUT:

117674609(PAM)

APRI:

131190111(PAM)

PTEX:

113448749(PAM)

NSS:	113415426(PAM)

VKO:	123031660(PAM)

PMUA:

114606841(PAM)

PRAF:

128423318(PAM)

ZVI:	118076918(PAM)

HCG:	128343029(PAM)

GJA:	107121208(PAM)

STOW:

125436547(PAM)

EMC:	129334290(PAM)

XLA:	379207(pam.L) 397736(pam.S)

XTR:	100170159(pam)

NPR:	108797815(PAM)

RTEM:

120931440(PAM)

BBUF:

120992549(PAM)

BGAR:

122924652(PAM)

MUO:	115463171(PAM)

GSH:	117352610(PAM)

DRE:	570822(pam)

SRX:	107715844 107737006

SANH:

107670040 107702561

SGH:	107558261(pam) 107560403

CCAR:

109097266 109098036

CAUA:

113053993 113109552

CGIB:

127966314 128021433

PTET:

122352678(pam)

LROH:

127171703(pam)

OMC:	131547997(pam)

PPRM:

120483160(pam)

MAMB:

125255806(pam)

CIDE:

127520520

MASI:

127432330 127433275

TROS:

130545926(pam)

TDW:	130411485(pam)

MANU:

129447594(pam)

IPU:

108277198

IFU:	128619989(pam)

PHYP:

113543505(pam)

SMEO:

124393835(pam)

TFD:	113639276(pam)

TVC:	132863745(pam)

AMEX:

103031431(pam)

CMAO:

118824741(pam)

EEE:	113576732(pam)

CHAR:

105894612(pam)

TRU:	101062613(pam)

TFS:	130516843(pam)

TNG:	GSTEN00013653G001

LCO:	104938829(pam)

TBEN:

117474446(pam)

CGOB:

115017065(pam)

PGEO:

117455911(pam)

GACU:

117557435(pam)

EMAC:

134868393(pam)

ELY:	117269835(pam)

EFO:	125905793(pam)

PLEP:

121960283(pam)

SLUC:

116041373(pam)

ECRA:

117958843(pam)

PFLV:

114570620(pam)

GAT:	120831508(pam)

PPUG:

119226240(pam)

AFB:	129102660(pam)

CLUM:

117740491(pam)

PSWI:

130205500(pam)

MSAM:

119914308(pam)

SCHU:

122865869(pam)

CUD:	121525000(pam)

ALAT:

119030296(pam)

MZE:	101484633(pam)

ONL:	100700800(pam)

OAU:	116319154(pam)

OLA:	101164528(pam)

OML:	112140341(pam)

CSAI:

133455498(pam)

XMA:	102227462(pam)

XCO:	114149523(pam)

XHE:	116724732(pam)

PRET:

103473165(pam)

PFOR:

103152322

PLAI:

106947015(pam)

PMEI:

106922489(pam)

GAF:	122819675(pam)

PPRL:

129379781(pam)

CVG:	107098487(pam)

CTUL:

119797739(pam)

GMU:	124872307(pam)

NFU:	107395057(pam)

KMR:	108246466(pam)

ALIM:

106522199(pam)

NWH:	119424389(pam)

AOCE:

111579145(pam)

MCEP:

124996364(pam)

CSEM:

103389193(pam)

POV:	109637635(pam)

SSEN:

122758574(pam)

HHIP:

117771409(pam)

HSP:	118098063(pam)

SMAU:

118284799(pam)

LCF:

108877750

SDU:	111221118(pam)

SLAL:

111659622(pam)

XGL:	120806189(pam)

HCQ:	109530660(pam)

SSCV:

125979803

SBIA:

133490609(pam)

PEE:	133414017(pam)

PTAO:

133465262(pam)

BPEC:

110161642(pam)

MALB:

109960744(pam)

BSPL:

114867236(pam)

SJO:	128379787(pam)

SASA:

106571984(pam)

STRU:

115160105(pam)

OTW:	112219988(pam)

OMY:	110523161(pam)

OGO:	123989512(pam)

ONE:	115118014(pam)

OKI:	109868844(pam)

OKE:	118387260(pam)

SALP:

111961960(pam)

SNH:	120025478(pam)

CCLU:

121581954(pam)

ELS:	105020460(pam)

SFM:	108929922(pam)

PKI:	111835769(pam)

AANG:

118212765(pam)

LOC:	102682623(pam)

PSPA:

121302893(pam) 121315078

ARUT:

117409376 117963343

PSEX:

120532663(pam)

LCM:	102348819(PAM)

CMK:	103178402(pam)

RTP:	109919477(pam)

CPLA:

122559877(pam)

HOC:	132823331(pam)

LERI:

129695861(pam)

PMRN:

116950708 116954932

LRJ:	133360938(PAM)

BFO:	118407264 118409685

BBEL:

109470824 109482227

CIN:

100186640

SCLV:

120333720 120334207

SPU:	579753 587198

LPIC:

129261976

APLC:

110987818

SKO:	100370314 100373175 102808073

DME:	Dmel_CG12130(Pal1) Dmel_CG5472(Pal2)

DER:	6541448 6547868

DSE:	6608435 6615755

DSI:	Dsimw501_GD10702(Dsim_GD10702) Dsimw501_GD11785(Dsim_GD11785)

DYA:	Dyak_GE14315 Dyak_GE19318

DAN:	6495917 6496404

DSR:	110177054 110180690

DPO:	4804533 4804713

DPE:	6592784 6593030

DMN:	108158680 108158961 117185951 117194126

DWI:	6641076 6652435

DGR:	6560913 6561212

DMO:	Dmoj_GI20014 Dmoj_GI20251

DAZ:	108616536 108616564

DNV:	108654373 108654454

DHE:	111593849 111600629

DVI:	6625579 6626018

CCAT:

101455585 101456470

BOD:	106620753 106624845

BDR:	105227732 105227807

RZE:	108361266 108376966

AOQ:	129245590 129247023

TDA:	119677971 119678536 119678831 119684223

MDE:	101897104 101897772

SCAC:

106080801 106091732

LCQ:	111681427 111681954

LSQ:	119600038 119609089

GFS:	119639718 119640309

ECOE:

129949407 129949782

CLON:

129611002 129611026

HIS:	119660118 119661513

AGA:	1269623 1276453

ACOZ:

120947392 120952938

AARA:

120893804 120894091

AMER:

121591391 121594156

ASTE:

118511439 118514168

AFUN:

125768688 125769331

AMOU:

128305296 128305443

AALI:

118467085 118467716

AAG:	5571279 5579975

AALB:

109417982 109622343

CQU:	CpipJ_CPIJ000256 CpipJ_CPIJ005687

CPII:

120427439 120428750

CNS:	116346954 116347859

BCOO:

119074112 119083604

AME:

408568

ACER:

108002175

ALAB:

122715792

ADR:

102671915

AFLR:

100869356

BIM:

100741817

BBIF:

117204104

BVK:

117239857

BVAN:

117158230

BTER:

100644430

BAFF:

126919397

BPYO:

122574499

BPAS:

132911018

FVI:

122534282

CCAL:

108630380

OBB:

114873553

OLG:

117601058

NMEA:

116426907

CGIG:

122405238

SOC:	105193621 105199939

MPHA:

105839940

AEC:	105153715 105154160

ACEP:

105622782 105626661

PBAR:

105424879 105429536

VEM:	105562634 105568363

HST:	105187606 105191264

DQU:	106741045 106741852

CFO:	105256161 105256681

FEX:	115236132 115241061

LHU:	105669317 105679657

PGC:	109860270 109862749

OBO:	105275266 105276820

PCF:	106788478 106788980

PFUC:

122520739 122522137

VPS:	122637433 122637841

VCRB:

124426015 124426021

VVE:	124946503 124957963

NVI:	100114818 100122955

CSOL:

105361409 105362823

TPRE:

106653543

LHT:

122503014

LBD:

127290501

MDL:	103569578 103570412

CGLO:

123259187 123268943

FAS:

105264633

DAM:	107038240 107043180

AGIF:

122850645 122853364

VCAN:

122412620 122415165

CCIN:

107266516 107275181

DSM:	124411322 124415995

NPT:	124218129 124218679

NFB:	124180068 124182996

NLO:	107225522 107227985

NVG:	124302555 124304458

AROA:

105684716 105690256

TCA:

657814

DPA:

109540523

SOY:	115885428 115890881

AGRG:

126742646

ATD:

109598830

CSET:

123308297

AGB:

108907151

LDC:

111506733

NVL:

108563948

APLN:

108737040

PPYR:

116158659 116164887

OTU:

111423876

BMOR:

101742958 105841291

BMAN:

114242070 114247451

MSEX:

115444747 115448582 119191969

DPL:	KGM_202935 KGM_215724

BANY:

112045179 112050125

MJU:

123876018

NIQ:	126770237 126775113

VCD:	124533534 124537644

MCIX:

123654480 123661861

PMAC:

106714150 106716757

PPOT:

106099339 106102385

PXU:	106114412 106124772

PRAP:

110991508 111001871

PBX:	123712966 123714504

PNAP:

125056789 125059953

ZCE:	119831604 119838812

CCRC:

123697346 123699103

LSIN:

126968700 126971950

HAW:	110370575 110372930

HZE:	124632347 124638216

TNL:	113501766 113504497

SLIU:

111349264 111353138

OFU:	114359644 114366202

PXY:	105387500 105390219 105395950

PGW:	126374659 126378508

CFEL:

113375565 113378033 113385684

CCRN:

123299196

API:	100162090 100166463

DNX:	107163745 107173682

AGS:	114128973 114132119

RMD:	113551314 113560512

ACOO:

126837348 126841143

DVT:	126893595 126904462

BTAB:

109031371 109035806 109036031

DCI:

103514212

CLEC:

106664759 106665330

HHAL:

106685326

NLU:	111046785 111049966 120348819

HVI:	124354564 124355488

MQU:	128996672 128997024 128997424

PHU:	Phum_PHUM262630 Phum_PHUM387350

FOC:	113213595 113217573

TPAL:

117642998 117647089

ZNE:	110830633 110837948

CSEC:

111862811 111866180

SGRE:

126271872

BROR:

134530494 134532643

IEL:	124165235 124165312

FCD:	110845914 110846222 110846791

DPX:	DAPPUDRAFT_327379 DAPPUDRAFT_330720

DMK:	116919032 116930770 123466817

DPZ:	124312136 124342993

PVM:	113800005 113815933 113815942

PJA:	122253825 122266205

PCHN:

125041365

PMOO:

119577876 119591754

HAME:

121866941 121870797

PCLA:

123745782 123757522

CQD:	128685583 128700964

PTRU:

123505879 123506128

ESN:	126980877 126988149 126993082

HAZT:

108671767

EAF:	111695161 111695791 111702393

LSM:	121121168 121129077

TCF:

131879040

PPOI:

119101386

DSV:	119433786 119442465

RSAN:

119405325

RMP:	119159463 119181443 119185517

TUT:	107362202 107370625

DPTE:

113796289 113799508

DFR:	124492876 124496248

CSCU:

111617460 111633922

PTEP:

107452529

ABRU:

129972994 129976626

UDV:

129229782

SDM:	118194459 118205964

LPOL:

106465021 106465024

PMEO:

129594725

CEL:	CELE_F21F3.1(pgal-1) CELE_T19B4.1(pamn-1)

CBR:	CBG_14919(Cbr-pamn-1) CBG_20492(Cbr-pgal-1)

CRQ:	GCK72_000272 GCK72_001307

NAI:	NECAME_00981 NECAME_06614

LOA:	LOAG_02126 LOAG_18084

TSP:

Tsp_08245

HRO:	HELRODRAFT_90150 HELRODRAFT_90186

LGI:	LOTGIDRAFT_128636 LOTGIDRAFT_204047

PVUL:

126817066 126818801

PCAN:

112553876 112554259

BGT:	106059574 106074305

GAE:

121386141

HRF:	124143750 124146994 124147038

HRJ:

124288942

CRG:

105320938

CVN:

111129912

CANU:

128175949

OED:

125652837

MYI:	110443164 110453761

PMAX:

117334163

MCAF:

127734323

MMER:

123565313

RPHI:

132759442

DPOL:

127876651 127880052

OBI:	106870427 106875056

OSN:

115218053

LAK:

106162187

SMM:	Smp_156530

SHX:	MS3_00006564

OVI:	T265_08837

EGL:

EGR_00446

NVE:	5505959 5513959

EPA:	110253509 110253517

ATEN:

116290559 116306504

ADF:

107333635

AMIL:

114961864 114967143

PDAM:

113668474 113672672

SPIS:

111322235

DGT:	114522559 114541151

XEN:	124452262 124454721

HMG:

100212200

HSY:	130640814 130643790 130646165

TAD:	TRIADDRAFT_63754

OLU:	OSTLU_15206(PAM1)

OTA:	OT_ostta04g04860

BPG:	Bathy08g04470

MIS:	MICPUN_70293

MPP:	MICPUCDRAFT_60313(JGI:MicpuC2_60313)

EMV:	HQR01_11890

PNS:	A9D12_10760

EAT:	EAT1b_2563

EXM:	U719_07590

STP:	Strop_2878

CATI:

CS0771_54180

ROO:	G5S37_12440

PSEG:

D3H65_32540

PGIN:

FRZ67_14645

FLS:	GLV81_02430

LACS:

H4075_12625

PSTY:

BFS30_16400

PGS:	CPT03_09935

SHT:	KO02_09560

SMIZ:

4412673_03632

MUH:	HYN43_024645

MRUB:

DEO27_026125

MGOS:

DIU38_029515

MMAB:

HQ865_19780

AGD:	FRZ59_06715

SBX:	CA265_01595

CMR:	Cycma_2166

CAMU:

CA2015_2102

FBC:	FB2170_01457

ZGA:	ZOBELLIA_2005

PHAL:

H9I45_14220

AALG:

AREALGSMS7_00469

AQD:	D1816_07170

FBE:	FF125_12995

» show all

Reference

1 [PMID:2207061]

Authors

Katopodis AG, Ping D, May SW.

Title

A novel enzyme from bovine neurointermediate pituitary catalyzes dealkylation of alpha-hydroxyglycine derivatives, thereby functioning sequentially with peptidylglycine alpha-amidating monooxygenase in peptide amidation.

Journal

Biochemistry 29:6115-20 (1990)
DOI:10.1021/bi00478a001

Reference

2 [PMID:9405058]

Authors

Bell J, Ash DE, Snyder LM, Kulathila R, Blackburn NJ, Merkler DJ

Title

Structural and functional investigations on the role of zinc in bifunctional rat peptidylglycine alpha-amidating enzyme.

Journal

Biochemistry 36:16239-46 (1997)
DOI:10.1021/bi970903d

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