KEGG ENZYME: 4.4.1.29

ENZYME: 4.4.1.29

Entry

EC 4.4.1.29                 Enzyme

Name

phycobiliprotein cysteine-84 phycobilin lyase;
cpcS (gene name);
cpeS (gene name);
cpcS1 (gene name);
cpcU (gene name);
phycocyanobilin:Cys-beta84-phycobiliprotein lyase

Class

Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)

Sysname

[phycobiliprotein]-Cys84-phycobilin:phycobilin lyase

Reaction(IUBMB)

(1) [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin;
(2) [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin;
(3) [allophycocyanin alpha-subunit]-Cys84-phycocyanobilin = apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin;
(4) [allophycocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[allophycocyanin beta-subunit] + (2R,3E)-phycocyanobilin;
(5) [C-phycoerythrin alpha-subunit]-Cys84-phycoerythrobilin = apo-[C-phycoerythrin alpha-subunit] + (2R,3E)-phycoerythrobilin;
(6) [C-phycoerythrin beta-subunit]-Cys84-phycoerythrobilin = apo-[C-phycoerythrin beta-subunit] + (2R,3E)-phycoerythrobilin

Substrate

[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin;
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin;
[allophycocyanin alpha-subunit]-Cys84-phycocyanobilin;
[allophycocyanin beta-subunit]-Cys84-phycocyanobilin;
[C-phycoerythrin alpha-subunit]-Cys84-phycoerythrobilin;
[C-phycoerythrin beta-subunit]-Cys84-phycoerythrobilin

Product

apo-[C-phycocyanin beta-subunit];
(2R,3E)-phycocyanobilin;
apo-[phycoerythrocyanin beta-subunit];
apo-[allophycocyanin alpha-subunit];
apo-[allophycocyanin beta-subunit];
apo-[C-phycoerythrin alpha-subunit];
(2R,3E)-phycoerythrobilin;
apo-[C-phycoerythrin beta-subunit]

Comment

The enzyme, found in cyanobacteria and red algae, catalyses the attachment of phycobilin chromophores to cysteine 84 of several phycobiliproteins (the numbering used here corresponds to the enzyme from Anabaena, in other organisms the number may vary slightly). It can attach phycocyanobilin to the beta subunits of C-phycocyanin and phycoerythrocyanin and to both subunits of allophycocyanin. In addition, it can attach phycoerythrobilin to both subunits of C-phycoerythrin.

History

EC 4.4.1.29 created 2015

Reference

1 [PMID:16452471]

Authors

Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H

Title

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120.

Journal

J Biol Chem 281:8573-81 (2006)
DOI:10.1074/jbc.M513796200

Sequence

[ana:alr0617]

Reference

2 [PMID:17726096]

Authors

Zhao KH, Su P, Tu JM, Wang X, Liu H, Ploscher M, Eichacker L, Yang B, Zhou M, Scheer H

Title

Phycobilin:cystein-84 biliprotein lyase, a near-universal lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins.

Journal

Proc Natl Acad Sci U S A 104:14300-5 (2007)
DOI:10.1073/pnas.0706209104

Sequence

[ana:alr0617]

Reference

3 [PMID:18199753]

Authors

Saunee NA, Williams SR, Bryant DA, Schluchter WM

Title

Biogenesis of phycobiliproteins: II. CpcS-I and CpcU comprise the heterodimeric bilin lyase that attaches phycocyanobilin to CYS-82 OF beta-phycocyanin and CYS-81 of allophycocyanin subunits in Synechococcus sp. PCC 7002.

Journal

J Biol Chem 283:7513-22 (2008)
DOI:10.1074/jbc.M708165200

Reference

4 [PMID:19864423]

Authors

Kupka M, Zhang J, Fu WL, Tu JM, Bohm S, Su P, Chen Y, Zhou M, Scheer H, Zhao KH

Title

Catalytic mechanism of S-type phycobiliprotein lyase: chaperone-like action and functional amino acid residues.

Journal

J Biol Chem 284:36405-14 (2009)
DOI:10.1074/jbc.M109.056382

Sequence

[ana:alr0617]