Entry
Name
pancreatic ribonuclease;
RNase;
RNase I;
RNase A;
pancreatic RNase;
ribonuclease I;
endoribonuclease I;
ribonucleic phosphatase;
alkaline ribonuclease;
ribonuclease;
gene S glycoproteins;
Ceratitis capitata alkaline ribonuclease;
SLSG glycoproteins;
gene S locus-specific glycoproteins;
S-genotype-asssocd. glycoproteins;
ribonucleate 3'-pyrimidino-oligonucleotidohydrolase
Class
Lyases;
Phosphorus-oxygen lyases;
Phosphorus-oxygen lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
RNA lyase ([RNA]-3'-cytidine/uridine-3'-phosphate and 5'-hydroxy-ribonucleotide-3'-[RNA] producing)
Reaction(IUBMB)
(1) an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] (overall reaction);
(1a) an [RNA] containing cytidine = an [RNA]-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA];
(1b) an [RNA]-3'-cytidine-2',3'-cyclophosphate + H2O = an [RNA]-3'-cytidine-3'-phosphate;
(2) an [RNA] containing uridine + H2O = an [RNA]-3'-uridine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA];
(2a) an [RNA] containing uridine = an [RNA]-3'-uridine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA];
(2b) an [RNA]-3'-uridine-2',3'-cyclophosphate + H2O = an [RNA]-3'-uridine-3'-phosphate
Substrate
[RNA] containing cytidine;
H2O [CPD:
C00001 ];
[RNA]-3'-cytidine-2',3'-cyclophosphate;
[RNA] containing uridine;
[RNA]-3'-uridine-2',3'-cyclophosphate
Product
[RNA]-3'-cytidine-3'-phosphate;
5'-hydroxy-ribonucleotide-3'-[RNA];
[RNA]-3'-cytidine-2',3'-cyclophosphate;
[RNA]-3'-uridine-3'-phosphate;
[RNA]-3'-uridine-2',3'-cyclophosphate
Comment
Specifically cleaves at the 3'-side of pyrimidine (uracil or cytosine) phosphate bonds in RNA. The reaction takes place in two steps, with the 2',3'-cyclic phosphodiester intermediates released from the enzyme at the completion of the first step. Hydrolysis of these cyclic compounds occurs at a much slower rate through a reversal of the first step, in which the -OH group of water substitutes for the 2'-OH group of the ribose used in the first step, and does not take place until essentially all the susceptible 3',5'-phosphodiester bonds have been cyclised. The enzyme can act as an endo- or exo ribonuclease.
History
EC 4.6.1.18 created 1972 as EC 3.1.4.22, transferred 1978 to EC 3.1.27.5, modified 1981, transferred 2018 to EC 4.6.1.18
Orthology
K01168 pancreatic ribonuclease
Genes
HSA : 6035(RNASE1) 6036(RNASE2)
PTR : 465207(RNASE1) 473325(RNASE2)
PPS : 100981889(RNASE2) 100995483(RNASE1)
GGO : 101145521(RNASE1) 101147913(RNASE2)
PON : 100173814(RNASE1) 100460680
NLE : 100586798 100591741 115830345 115832331
HMH : 116464874(RNASE1) 116485062
MCC : 704676(RNASE1) 706466(RNASE2)
MTHB : 126958320 126958329
MNI : 105496552 105496559(RNASE2)
CSAB : 103231361(RNASE1) 103231363(RNASE2)
CATY : 105593922(RNASE2) 105593924(RNASE1)
TGE : 112628662(RNASE1) 112629101(RNASE2)
MLEU : 105536738(RNASE1) 105536743(RNASE2)
RRO : 104669500 104672173 104672174 104672623(RNASE2)
RBB : 108515872 108516130(RNASE2) 108524692
TFN : 117070070(RNASE2) 117070071 117070072(RNASE1) 117074888
PTEH : 111538697 113223281 113223282 113223283
CANG : 105503838(RNASE2) 105503841(RNASE1) 105523134
CJC : 100389327(RNASE1) 100391488(RNASE3)
SBQ : 101031542 101031854(RNASE1)
CIMI : 108308153(RNASE2) 108308174(RNASE1)
CSYR : 103256741(RNASE1) 103258822 110595498
MMUR : 105864753(RNASE1) 105864771 105864773(RNASE2)
LCAT : 123621271(RNASE2) 123621299(RNASE1)
PCOQ : 105814183 105814296(RNASE1)
OGA : 100950392 100966234(RNASE1)
MMU : 19752(Rnase1) 53873(Ear7) 53877(Ear4) 54159(Rnase2b)
MCAL : 110288119 110309770 110309876(Rnase1) 110309912
MPAH : 110314765 110314852 110315578 110315658 110315720 110325813 110325870 110325927(Rnase1)
RNO : 100361909(Ear1) 305844(Rnase1l2) 364304(Rnase1) 474169(Rnase2)
MCOC : 116084498 116084688 116085216 116085217 116085223
ANU : 117705756 117705858 117705912 117705918 117705926 117706010
MUN : 110543247 110544166 110544500 110549725(Rnase1) 132656624 132656625 132656626 132656627
CGE : 100689288(Ear10) 100689289(Ear11) 100689291(Ear7) 100756274(Rnase1)
MAUA : 101834164(Rnase1) 101839975 121136362 121136368 121136373 121138486
PROB : 127216195 127216218(Rnase1)
PLEU : 114682269(Rnase1) 114682271 114682272
NGI : 103733657(Rnase1) 103733690
CPOC : 100716938 100717209 100720895
DORD : 105995816(Rnase2) 106000429(Rnase1)
MMMA : 107144333(Rnase1) 107145507
OCU : 100351089 103351154(RAF2) 103351156
TUP : 102476976 102482913(RNASE1) 102486685(RNASE2) 102487501 102496645 106736330
GVR : 103581606 103599933 103603911(RNASE1)
ELK : 111140359 111140693 111153368 111153369 111161666
LLV : 125105326 125105327 125105328 125105331 125105332
MPUF : 101672053 101672824 101679288 101687150(RNASE1)
MNP : 131998596 131998598(RNASE1) 132029232 132029233
MLK : 131835242 131836017 131836018 131836021 131836027(RNASE1) 131836028
NVS : 122892926 122893080 122893134 122893401 122893506
BTA : 100125948(RNASE2) 280720(BRB) 280930(RNASE1) 282340(RNASE1)
BOM : 102266201(RNASE1) 102285547(RNASE2) 102286022 102286301
BIU : 109564482(RNASE2) 109564483 109564861 109564864
BBUB : 102389674 102390334(RNASE2) 102415699
BBIS : 104988187 104988188 104988191
CHX : 102173194(RNASE2) 102173852 102174479 102174664
OAS : 101112062(RNASE2) 101112572 101112822 101113761
BTAX : 128054325 128054572
ODA : 120853345 120853347 120853348(RNASE2)
CCAD : 122443302 122443304 122443305
MBEZ : 129562646 129562783 129562971 129563164
SSC : 100312980(RNASE1) 102163838
CBAI : 105064664 105064665
CDK : 105097632 105097633 105097635
VPC : 102526523 102528688 102534153
BACU : 103015209(RNASE1) 103015486(RNASE2)
OOR : 101275695(RNASE1) 101275939
DLE : 111181593(RNASE2) 111181814(RNASE1)
PCAD : 102984671(RNASE1) 102985237
ECB : 100058995(RNASE1) 102148204
EPZ : 103542465(RNASE1) 103546041
EAI : 106833277(RNASE1) 123276424
MYB : 102242292(RNASE1) 102246040 102246159(RNASE2) 102254467 102255887 102258486
MMYO : 118664189 118664196 118664200(RNASE1)
MLF : 102420212 102421348 102421648 102422773 102431349 102433660 102440480 102442644
PKL : 118703904 118703956 118724348(RNASE1) 118724370
EFUS : 103302733 103302756(RNASE2) 103304773 129149164
HAI : 109374274 109376437(RNASE1) 109376442
DRO : 112311749(RNASE1) 112312002
SHON : 118982623(RNASE1) 118982639 118982648(RNASE2)
AJM : 119036557 119036692(RNASE1)
PDIC : 114490266 114490272 114490316(RNASE1) 118501025
PHAS : 123808395(RNASE1) 123808402
MMF : 118623404(RNASE2) 118637799
RFQ : 117023661 117024075(RNASE1)
PPAM : 129064121(RNASE1) 129064123(RNASE2) 129081880
PALE : 102878759(RNASE1) 102879014(RNASE2)
PGIG : 120608068(RNASE1) 120608099(RNASE2)
PVP : 105307291(RNASE1) 105307292(RNASE2)
RAY : 107521167(RNASE1) 107521168(RNASE2)
TOD : 119238001 119238208 119238209 119238212 119238213 119238863 119238867 119238868 119238870 119238871
SARA : 101555011(RNASE1) 101555279
LAV : 100671803 104847072(RNASE2)
MDO : 100023066 100029662 103102267 103104413
GAS : 123234126 123234129 123234130 123236045 123236046 123236052 123236898
SHR : 100931633 105749827 116421537
PCW : 110205810 110205870 110206608 110219094
ASN : 102371017 102375656 106723468 112548987
AMJ : 102568630(RNASE1) 102573106 106739133
BPEC : 110153686 110153688 110159162 110159169 110165503
ARUT : 117407735 117967204 117967205 117967293 117967294 117967295 117969234 117969235 117971032
» show all
Taxonomy
Reference
1
Authors
Anfinsen, C.B. and White, F.H., Jr.
Title
The ribonucleases: occurrence, structure, and properties.
Journal
In: Boyer, P.D., Lardy, H. and Myrback, K. (Eds.), The Enzymes, 2nd ed., vol. 5, Academic Press, New York, 1961, p. 95-122.
Reference
Authors
BEARD JR, RAZZELL WE.
Title
PURIFICATION OF ALKALINE RIBONUCLEASE II FROM MITOCHONDRIAL AND SOLUBLE FRACTIONS OF LIVER.
Journal
J Biol Chem 239:4186-93 (1964)
Reference
Authors
Cannistraro VJ, Kennell D.
Title
Purification and characterization of ribonuclease M and mRNA degradation in Escherichia coli.
Journal
Reference
Authors
Cuchillo CM, Pares X, Guasch A, Barman T, Travers F, Nogues MV
Title
The role of 2',3'-cyclic phosphodiesters in the bovine pancreatic ribonuclease A catalysed cleavage of RNA: intermediates or products?
Journal
Reference
Authors
Loverix S, Laus G, Martins JC, Wyns L, Steyaert J
Title
Reconsidering the energetics of ribonuclease catalysed RNA hydrolysis.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 4.6.1.18
ExPASy - ENZYME nomenclature database: 4.6.1.18