Entry
Name
N-(sulfonatooxy)alkenimidothioic acid sulfate-lyase (epithionitrile-forming);
ESP (gene name);
epithionitrile-specifier protein;
epithiospecifier protein
Class
Lyases;
Nitrogen-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
N-(sulfonatooxy)alkenimidothioic acid sulfate-lyase (epithionitrile-forming)
Reaction(IUBMB)
N-(sulfonatooxy)alkenimidothioic acid with a terminal double bond = an epithionitrile + sulfate [RN:
R13129 ]
Reaction(KEGG)
Substrate
N-(sulfonatooxy)alkenimidothioic acid with a terminal double bond
Product
Comment
The enzyme is involved in the breakdown of glucosinolates. It acts only on aliphatic N-(sulfonatooxy)alkenimidothioic acids produced from omega-alkenyl-glucosinolates, and forms epithionitrile-containing products. cf. EC
4.8.1.5 , thiohydroximate-O-sulfate sulfate/sulfur-lyase (nitrile-forming), and EC
4.8.1.7 , phenyl-N-(sulfonatooxy)methanimidothioate sulfolyase.
History
EC 4.8.1.6 created 2022
Orthology
K26381 N-(sulfonatooxy)alkenimidothioic acid sulfate-lyase (epithionitrile-forming)
Genes
BRP : 103832745 103871086 103871098
BNA : 106345817 106345821 106348445 106387979 106436486
Taxonomy
Reference
Authors
Lambrix V, Reichelt M, Mitchell-Olds T, Kliebenstein DJ, Gershenzon J.
Title
The Arabidopsis epithiospecifier protein promotes the hydrolysis of glucosinolates to nitriles and influences Trichoplusia ni herbivory.
Journal
Sequence
Reference
Authors
Zabala Mde T, Grant M, Bones AM, Bennett R, Lim YS, Kissen R, Rossiter JT.
Title
Characterisation of recombinant epithiospecifier protein and its over-expression in Arabidopsis thaliana.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.8.1.6
ExPASy - ENZYME nomenclature database: 4.8.1.6
BRENDA, the Enzyme Database: 4.8.1.6