KEGG   ENZYME: 5.1.3.27
Entry
EC 5.1.3.27                 Enzyme                                 
Name
dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase;
dTDP-deoxyglucose 3-epimerase;
dTDP-4-keto-6-deoxy-D-glucose 3-epimerase;
dTDP-4-keto-6-deoxyglucose 3-epimerase;
gerF (gene name);
tylJ (gene name);
chmJ (gene name);
mydH (gene name)
Class
Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives
Sysname
dTDP-4-dehydro-6-deoxy-alpha-D-glucose 3-epimerase
Reaction(IUBMB)
dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-gulose [RN:R06437]
Reaction(KEGG)
R06437
Substrate
dTDP-4-dehydro-6-deoxy-alpha-D-glucose [CPD:C11907]
Product
dTDP-4-dehydro-6-deoxy-alpha-D-gulose [CPD:C11926]
Comment
The enzyme is involved in the biosynthetic pathway of dTDP-6-deoxy-alpha-D-allose, which is converted to mycinose after attachment to the aglycones of several macrolide antibiotics, including tylosin, chalcomycin, dihydrochalcomycin, and mycinamicin II.
History
EC 5.1.3.27 created 2013
Pathway
ec00523  Polyketide sugar unit biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13312  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
K19855  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
Genes
STRMM444_29900
SDRZNEH16_32030
SGKPET44_28320
MCHNHCR76_04910
BEIGCM100_27600(rfbC)
NGVCDO52_02260
MFEUH1D33_25635(rfbC)
Reference
1  [PMID:15049360]
  Authors
Sohng JK, Kim HJ, Nam DH, Lim DO, Han JM, Lee HJ, Yoo JC
  Title
Cloning, expression, and biological function of a dTDP-deoxyglucose epimerase (gerF) gene from Streptomyces sp. GERI-155.
  Journal
Biotechnol Lett 26:185-91 (2004)
DOI:10.1023/b:bile.0000013709.80691.97
  Sequence
Reference
2  [PMID:17053005]
  Authors
Thuy TT, Liou K, Oh TJ, Kim DH, Nam DH, Yoo JC, Sohng JK
  Title
Biosynthesis of dTDP-6-deoxy-beta-D-allose, biochemical characterization of dTDP-4-keto-6-deoxyglucose reductase (GerKI) from Streptomyces sp. KCTC 0041BP.
  Journal
Glycobiology 17:119-26 (2007)
DOI:10.1093/glycob/cwl060
  Sequence
Reference
3  [PMID:23116432]
  Authors
Kubiak RL, Phillips RK, Zmudka MW, Ahn MR, Maka EM, Pyeatt GL, Roggensack SJ, Holden HM
  Title
Structural and functional studies on a 3'-epimerase involved in the biosynthesis  of dTDP-6-deoxy-D-allose.
  Journal
Biochemistry 51:9375-83 (2012)
DOI:10.1021/bi3012737
Other DBs
ExplorEnz - The Enzyme Database: 5.1.3.27
IUBMB Enzyme Nomenclature: 5.1.3.27
ExPASy - ENZYME nomenclature database: 5.1.3.27
BRENDA, the Enzyme Database: 5.1.3.27

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