Entry
Name
mannuronan 5-epimerase;
algG (gene name);
alginate epimerase;
C5-mannuronan epimerase;
mannuronan C-5-epimerase
Class
Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives
BRITE hierarchy
Sysname
[mannuronan]-beta-D-mannuronate 5-epimerase
Reaction(IUBMB)
[mannuronan]-beta-D-mannuronate = [alginate]-alpha-L-guluronate
Reaction(KEGG)
Substrate
[mannuronan]-beta-D-mannuronate
Product
[alginate]-alpha-L-guluronate
Comment
The enzyme epimerizes the C-5 bond in some beta-D-mannuronate residues in mannuronan, converting them to alpha-L-guluronate residues, and thus modifying the mannuronan into alginate. It is found in brown algae and alginate-producing bacterial species from the Pseudomonas and Azotobacter genera.
History
EC 5.1.3.37 created 2015
Pathway
ec00051 Fructose and mannose metabolism
Orthology
Genes
PAEB : NCGM1900_2739(algG)
PRE : PCA10_11830(algG) PCA10_38180(algG)
PALC : A0T30_03860 A0T30_11830
PMOL : CLJ08_08405 CLJ08_22875
PVD : CFBP1590__4282(algG)
PPRC : PFLCHA0_c10390(algG)
PMUD : NCTC8068_04326(algG)
PFW : PF1751_v1c09430(algG)
PEN : PSEEN3463 PSEEN4540(algG)
PPUU : PputUW4_04476(algG)
PSV : PVLB_04210 PVLB_15520
PALK : PSAKL28_08690 PSAKL28_31910
PSEC : CCOS191_0907(algG) CCOS191_2019
PSET : THL1_1263 THL1_3799
PALL : UYA_04860 UYA_15375
POJ : PtoMrB4_25890 PtoMrB4_46240(algG)
PGY : AWU82_19105 AWU82_28980
PSEJ : HNQ25_10115 HNQ25_19305
PTY : JWV26_01425 JWV26_15940(algG)
PTRT : HU722_0006140(algG)
PQI : KH389_10430 KH389_22270(algG)
PXN : HU772_004395(algG) HU772_009380
PPRG : HU725_004060(algG) HU725_014450
PMUY : KSS95_07450(algG) KSS95_11710
PMAM : KSS90_04810(algG) KSS90_09855
PTW : TUM18999_50170(algG)
PCAV : D3880_05520 D3880_05585
PHF : NLY38_05215(algG) NLY38_16770
PKJ : Q1W70_05495(algG) Q1W70_21890(algG)
PHOM : KJF94_01525(algG) KJF94_13620(algG)
PCHE : QYM18_12780(algG) QYM18_23060
PLG : NCTC10937_04288(algG)
FRI : FraEuI1c_0157 FraEuI1c_0161
» show all
Taxonomy
Reference
Authors
Franklin MJ, Chitnis CE, Gacesa P, Sonesson A, White DC, Ohman DE
Title
Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase.
Journal
Sequence
Reference
Authors
Morea A, Mathee K, Franklin MJ, Giacomini A, O'Regan M, Ohman DE
Title
Characterization of algG encoding C5-epimerase in the alginate biosynthetic gene cluster of Pseudomonas fluorescens.
Journal
Reference
Authors
Nyvall P, Corre E, Boisset C, Barbeyron T, Rousvoal S, Scornet D, Kloareg B, Boyen C
Title
Characterization of mannuronan C-5-epimerase genes from the brown alga Laminaria digitata.
Journal
Reference
Authors
Jain S, Franklin MJ, Ertesvag H, Valla S, Ohman DE
Title
The dual roles of AlgG in C-5-epimerization and secretion of alginate polymers in Pseudomonas aeruginosa.
Journal
Reference
Authors
Douthit SA, Dlakic M, Ohman DE, Franklin MJ
Title
Epimerase active domain of Pseudomonas aeruginosa AlgG, a protein that contains a right-handed beta-helix.
Journal
Reference
Authors
Wolfram F, Kitova EN, Robinson H, Walvoort MT, Codee JD, Klassen JS, Howell PL
Title
Catalytic mechanism and mode of action of the periplasmic alginate epimerase AlgG.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 5.1.3.37
ExPASy - ENZYME nomenclature database: 5.1.3.37