KEGG ENZYME: 5.4.1.3

ENZYME: 5.4.1.3

Entry

EC 5.4.1.3                  Enzyme

Name

2-methylfumaryl-CoA isomerase;
mesaconyl-CoA C1-C4 CoA transferase;
Mct

Class

Isomerases;
Intramolecular transferases;
Transferring acyl groups

Sysname

2-methylfumaryl-CoA 1,4-CoA-mutase

Reaction(IUBMB)

2-methylfumaryl-CoA = 3-methylfumaryl-CoA [RN:R09283]

Reaction(KEGG)

R09283

Substrate

2-methylfumaryl-CoA [CPD:C06028]

Product

3-methylfumaryl-CoA [CPD:C18323]

Comment

The enzyme, purified from the bacterium Chloroflexus aurantiacus, acts as an intramolecular CoA transferase and does not transfer CoA to free mesaconate. It is part of the 3-hydroxypropanoate cycle for carbon assimilation.

History

EC 5.4.1.3 created 2014

Pathway

ec00720

Carbon fixation pathways in prokaryotes

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K14470

2-methylfumaryl-CoA isomerase

Genes

PMUD:

NCTC8068_02062(frc_4)

PSET:

THL1_4232

PIE:	HU724_013975

MARI:

ACP86_19420

MANP:

EHN06_02530

ALKM:

NKI27_11380

ASEM:

NNL22_07735

STAW:

NCG89_13785

THIP:

N838_00830

GAI:	IMCC3135_16905(mct)

OME:	OLMES_2172

CBW:	RR42_s1516

BUZ:	AYM40_06765

TVL:	FAZ95_10115 FAZ95_35735

BPE:

BP0435

BPC:

BPTD_0450

BPER:

BN118_0286

BPET:

B1917_3384

BPEU:

Q425_33760

BPAR:

BN117_0457

BPA:

BPP0464

BBH:	BN112_2948

BBR:

BB0464

BBM:	BN115_0451

BBX:	BBS798_0461

ACHB:

DVB37_15020

PARK:

LSG25_05970

VAM:	C4F17_14125

ADN:	Alide_3213

ADK:	Alide2_1234

HYB:

Q5W_20080

HYN:	F9K07_11940

XYK:	GT347_20755

APP:	CAP2UW1_2507

MESW:

A9K65_031180

EMX:	FKV68_30910

RFV:	RFYW14_04034

VGO:	GJW-30_1_01671(fldA_2)

APOL:

K9D25_22035

MEE:	DA075_00045

ACUT:

MRB58_00800

PZU:

PHZ_c3026

PHB:	HYN04_06120

RLI:	RLO149_c031960

DSH:

Dshi_2494

RED:	roselon_02264

SUAL:

KDD17_12265

TOM:	BWR18_13435

RMM:	ROSMUCSMR3_03781(mct)

ROK:	RAK1035_2623

RID:	RIdsm_00212(bbsF_2)

ROH:	FIU89_16605(frc4)

SAGU:

CDO87_15525

THAA:

CFI11_02315

ROT:	FIV09_02235(frc2)

MALU:

KU6B_14500(mct)

RMAI:

MACH21_17130(mct)

PMEH:

JWJ88_18480

PVER:

E3U25_12545

RBG:	BG454_05635

HBC:	AEM38_02600

NAR:

Saro_1223

NPP:	PP1Y_AT31398 PP1Y_AT31978

NPN:	JI59_17595

NOT:	C7W88_08755

NOR:	FA702_12990

NGF:	FRF71_05170

NOG:	GKE62_16825

SAL:

Sala_2895

SPHK:

SKP52_19820

SPHP:

LH20_06650

SMAG:

AN936_15830

SMAZ:

LH19_02460

STER:

AOA14_14415

SGI:	SGRAN_3008

SPHQ:

BWQ93_19060

SPHO:

C3E99_10410

SPHX:

E5675_00370

SINA:

KNJ79_05725

SPHU:

SPPYR_3583

SPHD:

HY78_18265

SPKC:

KC8_19695

SPHA:

D3Y57_03960

SLAN:

GV829_02835

SPAP:

H3Z74_06275

SRAD:

LLW23_12465

SCH:	Sphch_0992

SYB:	TZ53_19645

SPHB:

EP837_03072

SPHT:

K426_13800

SYA:	A6768_05915

SCLO:

SCLO_1006520

SUFL:

FIL70_08820 FIL70_14450

SBAR:

H5V43_06490

CIJ:	WG74_11810

SLAA:

EUU25_06500

BLAS:

BSY18_3596

BMAA:

T8S45_12500

AEP:	AMC99_02359

ALH:	G6N82_07865

ARUE:

QQX03_00995

AMX:	AM2010_1644

PMAS:

NCF86_09185

ADO:	A6F68_00320(fldA_1)

TFV:	IDJ81_09105

EFV:	CHH26_00235

QCI:	NCF85_15360

QSP:	L1F33_02170

QSO:	IRL76_13690

QAR:	K3148_06855

QGE:	K3136_06950

QPS:	K3166_11285

ELI:

ELI_12030

ELQ:	Ga0102493_11760

ERK:	CD351_03090

ERR:	DVR09_01635

ERF:	FIU90_03320(fldA)

EMV:	HQR01_04835

PNS:	A9D12_05440

PORL:

BG023_11590

PHZ:	CHX26_03250

POT:	E2E27_01885

NTD:	EGO55_04520

AFLV:

QQW98_03940

RAP:	RHOA_5785(mct)

SHUM:

STHU_30030(mct)

SVC:	STVA_20880(mct)

STEL:

STAQ_26630(mct)

ABS:	AZOBR_p410036

ABQ:	ABAZ39_30400

ABF:	AMK58_26100

ATI:	AL072_22110

AZT:	TSH58p_19335

AZM:	DM194_21385

AARE:

D3093_29570

HADH:

FRZ61_46020

NACI:

NUH88_21205

THAR:

T8K17_09585

ERZ:	ER308_08210

MPA:

MAP_2406c

MAO:

MAP4_1417

MAVI:

RC58_07025

MAVU:

RE97_07025

MAV:

MAV_1574

MIT:

OCO_16360

MIA:

OCU_16560

MID:

MIP_02258

MYO:

OEM_14390

MCHI:

AN480_08065

MIR:

OCQ_14040

MMAL:

CKJ54_06925

MLP:

MLM_2882

MMAN:

MMAN_25340

MSA:	Mycsm_02339

MUL:

MUL_3896

MMI:

MMAR_4031

MMAE:

MMARE11_38380

MLI:	MULP_04196

MPSE:

MPSD_39900

MSHO:

MSHO_00120 MSHO_61630

MMC:

Mmcs_1977

MKM:

Mkms_2023

MJL:

Mjls_1957

MMM:

W7S_06865

MKN:	MKAN_06110

MYV:	G155_11820

MYE:	AB431_01015

MDX:	BTO20_23015

MSHG:

MSG_03617

MFJ:	MFLOJ_50560

MSTO:

MSTO_54780

MSIM:

MSIM_40220

MSAK:

MSAS_19040

MKU:	I2456_19120

MLW:	MJO58_19890

MXE:	MYXE_16820

MNV:	MNVI_03100

MPAG:

C0J29_22200

MNM:	MNVM_20030

MGOR:

H0P51_20175

MCOO:

MCOO_16820

MBAI:

MB901379_03512(frc_5)

MSEO:

MSEO_24150

MHEK:

JMUB5695_01748

MGAU:

MGALJ_06700

MLJ:	MLAC_10180

MBRD:

MBRA_31450

MMAM:

K3U93_17265

MSPG:

F6B93_16130

MOT:	LTS72_05120

MPAA:

MKK62_07580

MKY:	IWGMT90018_42210

MADI:

A7U43_19910

MWU:	PT015_09470

MSM:	MSMEG_2468

MSG:	MSMEI_2407

MSB:	LJ00_12285

MSN:	LI99_12285

MSH:	LI98_12290

MVA:

Mvan_2197

MGI:

Mflv_4144

MSP:	Mspyr1_34870

MCB:	Mycch_1953

MNE:	D174_11125

MYN:	MyAD_10910

MFT:	XA26_24360

MPHL:

MPHLCCUG_02140(fldA_3)

MVQ:

MYVA_2097

MLL:	B1R94_00700

MRH:	MycrhN_5794

MTHN:

4412656_01628(frc_7)

MHAS:

MHAS_01026(mct)

MDU:	MDUV_23040

MCHT:

MCHIJ_09340

MDR:	MDOR_13680

MAUU:

NCTC10437_02049(frc_6)

MMAG:

MMAD_21790

MMOR:

MMOR_42620

MFX:	MFAL_26480

MAIC:

MAIC_55280

MIJ:	MINS_06440

MALV:

MALV_15080

MTY:	MTOK_12220

MPSC:

MPSYJ_44710

MARZ:

MARA_18630

MGAD:

MGAD_17330

MHEV:

MHEL_20730

MSAR:

MSAR_17300

MANY:

MANY_37030

MAUB:

MAUB_54860

MPOF:

MPOR_25280

MPHU:

MPHO_14280

MMUC:

C1S78_011140

MMAT:

MMAGJ_54560

MBOK:

MBOE_50690

MFG:	K6L26_20530

MSEI:

MSEDJ_34070

MFLV:

NCTC10271_03318(frc_12)

MCEE:

MCEL_15590

MMON:

EWR22_10040

MGO:	AFA91_27155

MHOL:

K3U96_16765

MSEN:

K3U95_17970

MPAE:

K0O64_00650

MRF:	MJO55_09920

MDF:	K0O62_11125

MCRO:

MI149_01185

MGRO:

FZ046_16075

MKR:	MKOR_29920

MPAK:

MIU77_18320

MJD:	JDM601_4181

MTER:

4434518_04118(frc_11)

MMIN:

MMIN_16210

MHIB:

MHIB_34980

MHER:

K3U94_22800

MVM:	MJO54_22865

NNO:	NONO_c28540 NONO_c32800

RHA:	RHA1_ro02874

RER:

RER_51910

REY:

O5Y_24555

REB:	XU06_24975

RQI:	C1M55_26175

ROP:

ROP_26000

ROA:	Pd630_LPD07035

RPY:	Y013_15560

RBY:	CEJ39_02065

RAV:	AAT18_25020 AAT18_25220

RRZ:	CS378_14600 CS378_14800

RHU:	A3Q40_03009(mct)

RHQ:	IM25_05515

RHOD:

AOT96_05985

RRT:	4535765_04732(frc_13)

RCR:	NCTC10994_02797(frc_2)

RTM:	G4H71_12190

RKO:	JWS14_15115

RGO:	KYT97_16010

ROZ:	CBI38_28365

RPSK:

JWS13_29700

RGOR:

NMQ04_20225

GBR:

Gbro_4182

GPO:	GPOL_c45170

GOR:

KTR9_4108

GOQ:	ACH46_19985

GTA:	BCM27_21700

GOC:	CXX93_03660

GIT:	C6V83_01010

GRU:	GCWB2_20770(fldA6)

GOM:	D7316_02274(mct)

GAV:	C5O27_07270

GOD:	GKZ92_19970

GAM:	GII34_15840

GPD:	GII33_15275

GJI:	H1R19_20085

GOI:	LK459_02760

GHN:	MVF96_20770

GAMI:

IHQ52_00540

GMG:	NWF22_16155

GSI:	P5P27_18550

SPIN:

KV203_05340

TSD:	MTP03_07210 MTP03_14840(mct)

DIZ:	CT688_10225

DPC:	A6048_10565

DLU:	A6035_10075

DKN:	NHB83_09955

SCIN:

CP977_30950

SAKB:

K1J60_08820

YIA:	LO772_33375 LO772_34925

MIO:	AOA12_04730

LARI:

KI794_06445

SUBA:

LQ955_15165

BLY:	A2T55_01930

BLIN:

BLSMQ_0281

BRI:	FDF13_04985

BSPO:

L1F31_01550

GYU:	FE374_03300

NOI:	FCL41_02445

AEF:	GEV26_00915

ASEZ:

H9L21_11795

TCU:

Tcur_4637

ACTW:

F7P10_36610

AMAZ:

LUW76_41975

ACIR:

OG979_04365

SPIQ:

OHA34_37775

ACTQ:

OG417_31730

NOW:	GBF35_13590

NCX:	Nocox_12120(frc3)

TBI:

Tbis_2092

FRE:	Franean1_6828

FRI:	FraEuI1c_2687

SACG:

FDZ84_27925

AMQ:	AMETH_3799 AMETH_4503

AMYC:

CU254_18695

AACD:

LWP59_31300

ARHD:

VSH64_11445

PDX:

Psed_1949

PSEE:

FRP1_10200

PSEH:

XF36_06540

PSEQ:

AD006_17750 AD006_31860

PECQ:

AD017_25585 AD017_28385

PAUT:

Pdca_37090

PBRO:

HOP40_32820

PPEL:

H6H00_19855

PMAD:

BAY61_15145

SACE:

GIY23_17080

PSUU:

Psuf_001410(mct)

BSOL:

FSW04_02105

CWO:

Cwoe_4298

SBAE:

DSM104329_02454(mct)

RRS:	RoseRS_4174

RCA:

Rcas_0911

CAU:

Caur_0175

CHL:	Chy400_0188

CAG:

Cagg_1329

GGR:	HKW67_06655

» show all

Reference

1 [PMID:19955419]

Authors

Zarzycki J, Brecht V, Muller M, Fuchs G

Title

Identifying the missing steps of the autotrophic 3-hydroxypropionate CO2 fixation cycle in Chloroflexus aurantiacus.

Journal

Proc Natl Acad Sci U S A 106:21317-22 (2009)
DOI:10.1073/pnas.0908356106

Sequence

[cau:Caur_0175]

Other DBs

ExplorEnz - The Enzyme Database:

5.4.1.3

IUBMB Enzyme Nomenclature:

5.4.1.3

ExPASy - ENZYME nomenclature database:

5.4.1.3

BRENDA, the Enzyme Database:

5.4.1.3

DBGET integrated database retrieval system