KEGG ENZYME: 6.1.2.1

ENZYME: 6.1.2.1

Entry

EC 6.1.2.1                  Enzyme

Name

D-alanine---(R)-lactate ligase;
VanA;
VanB;
VanD

Class

Ligases;
Forming carbon-oxygen bonds;
acid-alcohol ligases (ester synthases)

Sysname

D-alanine:(R)-lactate ligase (ADP-forming)

Reaction(IUBMB)

D-alanine + (R)-lactate + ATP = D-alanyl-(R)-lactate + ADP + phosphate [RN:R09588]

Reaction(KEGG)

R09588

Substrate

D-alanine [CPD:C00133];
(R)-lactate [CPD:C00256];
ATP [CPD:C00002]

Product

D-alanyl-(R)-lactate [CPD:C19694];
ADP [CPD:C00008];
phosphate [CPD:C00009]

Comment

The product of this enzyme, the depsipeptide D-alanyl-(R)-lactate, can be incorporated into the peptidoglycan pentapeptide instead of the usual D-alanyl-D-alanine dipeptide, which is formed by EC 6.3.2.4, D-alanine---D-alanine ligase. The resulting peptidoglycan does not bind the glycopeptide antibiotics vancomycin and teicoplanin, conferring resistance on the bacteria.

History

EC 6.1.2.1 created 2010

Orthology

K15739

D-alanine---(R)-lactate ligase

Genes

ASED:

IRT44_16970(vanA)

BAYD:

BSPP4475_08375(vanA)

PLV:	ERIC2_c07960(vanA)

PSON:

JI735_32490(vanA)

PMAE:

LMZ02_20975(vanA)

TVU:	AB849_010220(vanA)

LAH:	LA20533_00580

EFA:	EF2294(vanB)

EFC:	EFAU004_02775(vanB)

EFAU:

EFAU085_00735(vanB)

ESG:	EsVE80_p1-00080(vanA)

CARG:

RSJ17_20565

RGV:	NQ502_10130(vanD)

DSY:

DSY3690

DHD:

Dhaf_1664

DDL:	Desdi_1473

FWA:	DCMF_02700(vanB)

BLAR:

LC508_18525(vanD)

AMIC:

Ami3637_13565(vanA)

LCG:	L3BBH23_19240(vanB)

OLS:

Olsu_1201

TIO:	INP52_03930

NTP:	CRH09_30840(vanB)

NWL:	NWFMUON74_45700

TPR:

Tpau_0523

TOY:	FO059_01075(vanA)

SCO:	SCO3595(vanB)

SLV:	SLIV_20320

SXI:	SXIM_54620

STRC:

AA958_28375(vanB)

SLS:	SLINC_1844

SAQU:

EJC51_03785(vanA)

SBY:	H7H31_16175(vanA)

SRIM:

CP984_39910(vanA)

SSPB:

CP982_31320(vanA-Sc)

SROI:

IAG44_39585(vanA-Sc)

SSPN:

LXH13_34735(vanA-Sc)

SCOA:

QU709_45505(vanA-Sc)

STRZ:

OYE22_15920(vanA)

YIA:	LO772_06590(vanA)

RTX:	TI83_01670(vanB)

RTC:	APU90_04820(vanB)

RTN:	A6122_0299

RRY:	C1O28_00080(vanA)

RIA:	C7V51_10855(vanA)

AGG:	C1N71_08265

ASUN:

KG104_11150

NARO:

CFH99_00255(vanB)

KFL:

Kfla_3620

KQI:	F1D05_26435(vanA)

KSL:	OG809_31005(vanA)

ACTW:

F7P10_09890(vanA)

SPIQ:

OHA34_26520(vanO)

FRE:	Franean1_7137

AMD:	AMED_8289(ddlA)

AMN:	RAM_42565(vanB)

AMM:	AMES_8162(ddlA)

AMZ:	B737_8163(ddlA)

AOI:	AORI_1472(ddl)

AJA:	AJAP_32170

AMYC:

CU254_05730(vanB)

AMYB:

BKN51_41395(vanB)

AAB:	A4R43_21435(vanB)

AMYY:

YIM_36535(vanB)

AORI:

SD37_33760(vanB)

APRT:

MUY14_04915

ATHM:

L1857_33140(vanA-Sc)

SESP:

BN6_49760

SSYI:

EKG83_05355(vanA)

KAL:

KALB_1172

KUT:	JJ691_70650(ddlA_1)

KBU:	Q4V64_46365(vanA-Sc)

KPHY:

AOZ06_08680(vanB) AOZ06_28865(vanB)

ACTU:

Actkin_02323(vanB)

CROS:

N8J89_03400(vanA)

AOU:	ACTOB_006505(vanA)

PSUU:

Psuf_054820

ATL:	Athai_15820

ASER:

Asera_58740

DVC:	Dvina_26165(vanA)

DMAT:

Dmats_21695(vanA)

CAI:

Caci_5793

SNA:

Snas_5356

GLY:	K3N28_22515(vanA)

CWO:

Cwoe_1414

IAM:	HC251_07060

DHI:	LH044_08545(vanA)

KBS:	EPA93_09965(vanA)

KBB:	ccbrp13_15920(vanB)

» show all

Reference

1 [PMID:1931965]

Authors

Bugg TD, Wright GD, Dutka-Malen S, Arthur M, Courvalin P, Walsh CT

Title

Molecular basis for vancomycin resistance in Enterococcus faecium BM4147: biosynthesis of a depsipeptide peptidoglycan precursor by vancomycin resistance proteins VanH and VanA.

Journal

Biochemistry 30:10408-15 (1991)
DOI:10.1021/bi00107a007

Sequence

[ag:CAA40215]

Reference

2 [PMID:7957913]

Authors

Meziane-Cherif D, Badet-Denisot MA, Evers S, Courvalin P, Badet B

Title

Purification and characterization of the VanB ligase associated with type B vancomycin resistance in Enterococcus faecalis V583.

Journal

FEBS Lett 354:140-2 (1994)
DOI:10.1016/0014-5793(94)01096-X