KEGG ENZYME: 6.2.1.35

ENZYME: 6.2.1.35

Entry

EC 6.2.1.35                 Enzyme

Name

acetate---[acyl-carrier protein] ligase;
HS-acyl-carrier protein:acetate ligase;
[acyl-carrier protein]:acetate ligase;
MadH;
ACP-SH:acetate ligase

Class

Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases

Sysname

acetate:[acyl-carrier-protein] ligase (AMP-forming)

Reaction(IUBMB)

ATP + acetate + an [acyl-carrier protein] = AMP + diphosphate + an acetyl-[acyl-carrier protein] [RN:R09475]

Reaction(KEGG)

R09475

Substrate

ATP [CPD:C00002];
acetate [CPD:C00033];
[acyl-carrier protein] [CPD:C00229]

Product

AMP [CPD:C00020];
diphosphate [CPD:C00013];
acetyl-[acyl-carrier protein] [CPD:C03939]

Comment

This enzyme, from the anaerobic bacterium Malonomonas rubra, is a component of the multienzyme complex EC 7.2.4.4, biotin-dependent malonate decarboxylase. The enzyme uses the energy from hydrolysis of ATP to convert the thiol group of the acyl-carrier-protein-bound 2'-(5-phosphoribosyl)-3'-dephospho-CoA cofactor into its acetyl thioester [2].

History

EC 6.2.1.35 created 2008, modified 2018

Orthology

K20415

ACP-SH:acetate ligase

Genes

EFE:

EFER_3132

KPZ:	KPNIH27_22450

KPB:	FH42_14180

KLW:	DA718_03330

KGR:	JJJ10_02965

KPAS:

LUW96_03555

KLC:	K7H21_02975

KLM:	BWI76_25015

CAMA:

F384_01150

CTEL:

GBC03_24705

SFO:	Z042_24840

RPB:

RPB_3294

RPC:

RPC_1061

RCP:	RCAP_rcp00126

RAP:	RHOA_4942(madH)

ATI:	AL072_31140

AZZ:	DEW08_20125

RRU:

Rru_A1326

RRF:

F11_06860

DES:	DSOUD_0384

PFT:

JBW_01394

» show all

Reference

1 [PMID:1628643]

Authors

Hilbi H, Dehning I, Schink B, Dimroth P

Title

Malonate decarboxylase of Malonomonas rubra, a novel type of biotin-containing acetyl enzyme.

Journal

Eur J Biochem 207:117-23 (1992)
DOI:10.1111/j.1432-1033.1992.tb17028.x

Reference

2 [PMID:8664258]

Authors

Berg M, Hilbi H, Dimroth P

Title

The acyl carrier protein of malonate decarboxylase of Malonomonas rubra contains 2'-(5"-phosphoribosyl)-3'-dephosphocoenzyme A as a prosthetic group.

Journal

Biochemistry 35:4689-96 (1996)
DOI:10.1021/bi952873p

Sequence

[ag:AAC45404]

Reference

3 [PMID:9128730]

Authors

Berg M, Hilbi H, Dimroth P

Title

Sequence of a gene cluster from Malonomonas rubra encoding components of the malonate decarboxylase Na+ pump and evidence for their function.

Journal

Eur J Biochem 245:103-15 (1997)
DOI:10.1111/j.1432-1033.1997.00103.x

Sequence

[ag:AAC45404]

Reference

4 [PMID:11902724]

Authors

Dimroth P, Hilbi H

Title

Enzymic and genetic basis for bacterial growth on malonate.

Journal

Mol Microbiol 25:3-10 (1997)
DOI:10.1046/j.1365-2958.1997.4611824.x

Other DBs

ExplorEnz - The Enzyme Database:

6.2.1.35

IUBMB Enzyme Nomenclature:

6.2.1.35

ExPASy - ENZYME nomenclature database:

6.2.1.35

BRENDA, the Enzyme Database:

6.2.1.35

DBGET integrated database retrieval system