Entry Name [butirosin acyl-carrier protein]---L-glutamate ligase;
Class Ligases;BRITE hierarchy
Sysname [BtrI acyl-carrier protein]:L-glutamate ligase (ADP-forming)
Reaction(IUBMB) (1) ATP + L-glutamate + BtrI acyl-carrier protein = ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein] [RN:
R08905 ];
(2) ATP + L-glutamate + 4-amino butanoyl-[BtrI acyl-carrier protein] = ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] [RN:
R08906 ]
Reaction(KEGG) Substrate ATP [CPD:
C00002 ];
L-glutamate [CPD:
C00025 ];
BtrI acyl-carrier protein;
4-amino butanoyl-[BtrI acyl-carrier protein]
Product ADP [CPD:
C00008 ];
phosphate [CPD:
C00009 ];
L-glutamyl-[BtrI acyl-carrier protein];
4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
Comment Catalyses two steps in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family. The enzyme adds one molecule of L-glutamate to a dedicated acyl-carrier protein, and following decarboxylation of the product by EC
4.1.1.95 , L-glutamyl-[BtrI acyl-carrier protein] decarboxylase, adds a second L-glutamate molecule.
Requires Mg2+ or Mn2+, and activity is enhanced in the presence of Mn2+.
History EC 6.2.1.39 created 2012
Pathway ec00524 Neomycin, kanamycin and gentamicin biosynthesis
ec01110 Biosynthesis of secondary metabolites
Orthology K13559 [BtrI acyl-carrier protein]--L-glutamate ligase
Reference Authors Li Y, Llewellyn NM, Giri R, Huang F, Spencer JB
Title Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway.
Journal Sequence Other DBs ExplorEnz - The Enzyme Database: 6.2.1.39
ExPASy - ENZYME nomenclature database: 6.2.1.39
