Entry
Name
D-alanine---[D-alanyl-carrier protein] ligase;
dltA (gene name);
Dcl
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
D-alanine:[D-alanyl-carrier protein] ligase
Reaction(IUBMB)
ATP + D-alanine + holo-[D-alanyl-carrier protein] = AMP + diphosphate + D-alanyl-[D-alanyl-carrier protein] (overall reaction);
(1a) ATP + D-alanine = (D-alanyl)adenylate + diphosphate;
(1b) (D-alanyl)adenylate + holo-[D-alanyl-carrier protein] = AMP + D-alanyl-[D-alanyl-carrier protein]
Substrate
ATP [CPD:
C00002 ];
D-alanine [CPD:
C00133 ];
holo-[D-alanyl-carrier protein];
(D-alanyl)adenylate
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
D-alanyl-[D-alanyl-carrier protein];
(D-alanyl)adenylate
Comment
The enzyme is involved in the modification of wall teichoic acids, as well as type I and IV lipoteichoic acids, with D-alanine residues. It activates D-alanine using ATP to form a high-energy (D-alanyl)adenylate intermediate and subsequently transfers the alanyl moiety to the covalently-bound phosphopantheinyl cofactor of a D-alanyl-carrier protein (DltC).
History
EC 6.2.1.54 created 2018
Reference
Authors
Perego M, Glaser P, Minutello A, Strauch MA, Leopold K, Fischer W
Title
Incorporation of D-alanine into lipoteichoic acid and wall teichoic acid in Bacillus subtilis. Identification of genes and regulation.
Journal
Sequence
Reference
Authors
Yonus H, Neumann P, Zimmermann S, May JJ, Marahiel MA, Stubbs MT
Title
Crystal structure of DltA. Implications for the reaction mechanism of non-ribosomal peptide synthetase adenylation domains.
Journal
Sequence
Reference
Authors
Du L, He Y, Luo Y
Title
Crystal structure and enantiomer selection by D-alanyl carrier protein ligase DltA from Bacillus cereus.
Journal
Sequence
Reference
Authors
Osman KT, Du L, He Y, Luo Y
Title
Crystal structure of Bacillus cereus D-alanyl carrier protein ligase (DltA) in complex with ATP.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.54
ExPASy - ENZYME nomenclature database: 6.2.1.54