Entry
Name
long-chain fatty acid adenylase/transferase FadD23;
fadD23 (gene name);
long-chain fatty acid adenylyltransferase FadD23
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
palmitate:holo-[(hydroxy)phthioceranic acid synthase] ligase
Reaction(IUBMB)
(1) ATP + stearate + a holo-[(hydroxy)phthioceranic acid synthase] = AMP + diphosphate + a stearoyl-[(hydroxy)phthioceranic acid synthase] (overall reaction);
(1a) ATP + stearate = diphosphate + (stearoyl)adenylate [RN:
R12393 ];
(1b) (stearoyl)adenylate + a holo-[(hydroxy)phthioceranic acid synthase] = AMP + a stearoyl-[(hydroxy)phthioceranic acid synthase];
(2) ATP + palmitate + a holo-[(hydroxy)phthioceranic acid synthase] = AMP + diphosphate + a palmitoyl-[(hydroxy)phthioceranic acid synthase] (overall reaction);
(2a) ATP + palmitate = diphosphate + (palmitoyl)adenylate [RN:
R12396 ];
(2b) (palmitoyl)adenylate + a holo-[(hydroxy)phthioceranic acid synthase] = AMP + a palmitoyl-[(hydroxy)phthioceranic acid synthase]
Reaction(KEGG)
Substrate
ATP [CPD:
C00002 ];
stearate [CPD:
C01530 ];
holo-[(hydroxy)phthioceranic acid synthase];
(stearoyl)adenylate [CPD:
C22132 ];
palmitate [CPD:
C00249 ];
(palmitoyl)adenylate [CPD:
C22135 ]
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
stearoyl-[(hydroxy)phthioceranic acid synthase] [CPD:
C22134 ];
(stearoyl)adenylate [CPD:
C22132 ];
palmitoyl-[(hydroxy)phthioceranic acid synthase] [CPD:
C22124 ];
(palmitoyl)adenylate [CPD:
C22135 ]
Comment
This mycobacterial enzyme activates palmitate and stearate by adenylation, followed by their loading onto the polyketide synthase EC
2.3.1.287 , phthioceranic/hydroxyphthioceranic acid synthase.
History
EC 6.2.1.57 created 2019
Orthology
K12425 long-chain fatty acid adenylase/transferase FadD23
Genes
MTO : MTCTRI2_3905(fadD23)
MBO : BQ2027_MB3856(fadD23)
MGAU : MGALJ_58670(fadD23)
» show all
Taxonomy
Reference
Authors
Gokhale RS, Saxena P, Chopra T, Mohanty D
Title
Versatile polyketide enzymatic machinery for the biosynthesis of complex mycobacterial lipids.
Journal
Reference
Authors
Lynett J, Stokes RW
Title
Selection of transposon mutants of Mycobacterium tuberculosis with increased macrophage infectivity identifies fadD23 to be involved in sulfolipid production and association with macrophages.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.57
ExPASy - ENZYME nomenclature database: 6.2.1.57