KEGG   ENZYME: 6.2.1.62
Entry
EC 6.2.1.62                 Enzyme                                 
Name
3,4-dihydroxybenzoate---[aryl-carrier protein] ligase;
asbC (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
3,4-dihydroxybenzoate:[aryl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + 3,4-dihydroxybenzoate + holo-[aryl-carrier protein] = AMP + diphosphate + 3,4-dihydroxybenzoyl-[aryl-carrier protein] (overall reaction) [RN:R12575];
(1a) ATP + 3,4-dihydroxybenzoate = diphosphate + (3,4-dihydroxybenzoyl)adenylate [RN:R12576];
(1b) (3,4-dihydroxybenzoyl)adenylate + holo-[aryl-carrier protein] = AMP + 3,4-dihydroxybenzoyl-[aryl-carrier protein] [RN:R12577]
Reaction(KEGG)
R12575 R12576 R12577
Substrate
ATP [CPD:C00002];
3,4-dihydroxybenzoate [CPD:C00230];
holo-[aryl-carrier protein] [CPD:C20665];
(3,4-dihydroxybenzoyl)adenylate [CPD:C20884]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
3,4-dihydroxybenzoyl-[aryl-carrier protein] [CPD:C20883];
(3,4-dihydroxybenzoyl)adenylate [CPD:C20884]
Comment
The adenylation domain of the enzyme catalyses the activation of 3,4-dihydroxybenzoate to (3,4-dihydroxybenzoyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of an aryl-carrier protein domain. The aryl-carrier protein domain may be part of the same protein, or of a different protein. This activity is often found as part of a larger non-ribosomal peptide synthase.
History
EC 6.2.1.62 created 2020
Pathway
ec01053  Biosynthesis of siderophore group nonribosomal peptides
Orthology
K24110  3,4-dihydroxybenzoate---[aryl-carrier protein] ligase
Genes
GKD: K6Q96_02925
MAQ: Maqu_3524
MHC: MARHY3424
MARJ: MARI_31200(menE_2)
MALL: PBN92_17845
MSHE: MAALD49_35060
AMAC: MASE_09720
AMG: AMEC673_09965
ASP: AOR13_248
ASQ: AVL57_17230
AAW: AVL56_16115
ALE: AV939_16170
ALZ: AV940_15925
CATE: C2869_06590
HMI: soil367_15780
CATT: OLW01_08295
CELL: CBR65_18740
GIL: NHM04_07030
MKE: OOT55_16125
STAW: NCG89_14200
MII: BTJ40_19050
OAI: OLEAN_C24210
BACZ: KFF03_16070
OME: OLMES_0537
OCE: GU3_03570
AAK: AA2016_0178
AMIH: CO731_00651(fadD_1)
AMIS: Amn_05660
ANJ: AMD1_0673(fadD)
SFH: SFHH103_01318
EAD: OV14_a1319
EAH: FA04_19980
ECAA: J3R84_21200
ENP: JVX98_04515
AVQ: HRR99_17400
RPA: TX73_012335
RPB: RPB_4394
RPT: Rpal_2630
RPX: Rpdx1_3183
NWI: Nwi_2300
CMET: K6K41_17350
AUA: M673_21065
PGA: PGA1_78p00430
PGL: PGA2_95p520
PGD: Gal_04240
LMD: METH_16725
ALI: AZOLI_p20606
AZM: DM194_19470
SKT: IGS68_11030
SMUC: JL100_015625
SROE: JL101_010045
BAN: BA_1983(asbC)
BAR: GBAA_1983(asbC)
BAT: BAS1840
BAH: BAMEG_2607 BAMEG_2608(asbC)
BAI: BAA_2051(asbC)
BAX: H9401_1880
BANT: A16_20200
BANR: A16R_20440
BANS: BAPAT_1896
BANH: HYU01_09935
BANV: DJ46_788
BCE: BC1980
BCZ: BCE33L1797
BCB: BCB4264_A1985(asbC)
BCU: BCAH820_2018(asbC)
BCG: BCG9842_B3344(asbC)
BCX: BCA_2045(asbC)
BAL: BACI_c19570
BCF: bcf_09760
BTK: BT9727_1814
BTL: BALH_1755
BTB: BMB171_C1767(asbC)
BTT: HD73_2149
BTHR: YBT1520_10720
BTHI: BTK_10860
BTC: CT43_CH1936(asbC)
BTM: MC28_1186
BTG: BTB_c20500(asbC)
BTI: BTG_10045
BTN: BTF1_07290
BTHT: H175_ch1962
BTHU: YBT1518_11030
BTW: BF38_3197
BTHY: AQ980_20255
BWE: BcerKBAB4_1846
BWW: bwei_3033(asbC)
BMYO: BG05_4003
BTY: Btoyo_4554
BBY: CY96_08995
BWD: CT694_10810
BTRO: FJR70_09680(asbC)
BMOB: MLA2C4_10085
BNT: GSN03_09685(asbC)
BALU: QRY64_12030(asbC)
BWH: A9C19_17990
BARD: QRY57_11170
BHK: B4U37_11085
BCL: ABC1949
BLE: BleG1_1612
SHUA: PQ477_16910
BPF: BpOF4_08935(asbC)
BON: A361_02375
CFIR: NAF01_02370
CPSS: M5V91_19810
LSP: Bsph_2961
LFU: HR49_11805
LLB: R6U77_05295
MLIT: KDJ21_008470
MENL: MVE64_10285
MEBZ: LPC09_22170
ECTO: MUG87_15620
ALKL: MM271_23435
EXM: U719_06045
EXU: ESP131_08440
EACE: KKI46_06235
BBE: BBR47_20470
BLR: BRLA_c008690
BFM: BP422_05350
BRW: GOP56_20345
PMS: KNP414_07054
PMQ: PM3016_6643
PMW: B2K_33565
PTRI: KDC22_17570
PALR: HGI30_15375 HGI30_17890
CCHL: FPL14_04890 FPL14_24320
SOB: CSE16_06620
 » show all
Reference
1  [PMID:17346033]
  Authors
Pfleger BF, Lee JY, Somu RV, Aldrich CC, Hanna PC, Sherman DH
  Title
Characterization and analysis of early enzymes for petrobactin biosynthesis in Bacillus anthracis.
  Journal
Biochemistry 46:4147-57 (2007)
DOI:10.1021/bi6023995
  Sequence
[ban:BA_1983]
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.62
IUBMB Enzyme Nomenclature: 6.2.1.62
ExPASy - ENZYME nomenclature database: 6.2.1.62
BRENDA, the Enzyme Database: 6.2.1.62

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Pathway (2)   
   KEGG PATHWAY (2)   
Chemical substance (7)   
   KEGG COMPOUND (7)   
Chemical reaction (3)   
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Gene (219)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (130)   
   KEGG MGENES (88)   
Protein sequence (3)   
   UniProt (2)   
   SWISS-PROT (1)   
Protein domain (5)   
   InterPro (5)   
All databases (239)   

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