Entry
Name
L-arginine---[L-arginyl-carrier protein] ligase;
vabF (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
L-arginine:[L-arginyl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-arginine + holo-[L-arginyl-carrier protein] = AMP + diphosphate + L-arginyl-[L-arginyl-carrier protein] (overall reaction);
(1a) ATP + L-arginine = diphosphate + (L-arginyl)adenylate [RN:
R12572 ];
(1b) (L-arginyl)adenylate + holo-[L-arginyl-carrier protein] = AMP + L-arginyl-[L-arginyl-carrier protein]
Reaction(KEGG)
Substrate
ATP [CPD:
C00002 ];
L-arginine [CPD:
C00062 ];
holo-[L-arginyl-carrier protein];
(L-arginyl)adenylate [CPD:
C22264 ]
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
L-arginyl-[L-arginyl-carrier protein];
(L-arginyl)adenylate [CPD:
C22264 ]
Comment
The adenylation domain of the enzyme catalyses the activation of L-arginine to (L-arginyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of a peptidyl-carrier protein domain. The peptidyl-carrier protein domain may be part of the same protein, or of a different protein. This activity is often found as part of a larger non-ribosomal peptide synthase.
History
EC 6.2.1.63 created 2020
Orthology
K24101 L-arginine---[L-arginyl-carrier protein] ligase
Genes
SFJ : SAMEA4384070_1829(dhbF_3)
VSR : Vspart_01407(lgrD_3)
ABS : AZOBR_p350078 AZOBR_p350079
» show all
Taxonomy
Reference
Authors
Balado M, Osorio CR, Lemos ML
Title
A gene cluster involved in the biosynthesis of vanchrobactin, a chromosome-encoded siderophore produced by Vibrio anguillarum.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.63
ExPASy - ENZYME nomenclature database: 6.2.1.63