Entry
Name
E1 NEDD8-activating enzyme;
NEDD-activating enzyme E1;
NAE1 (gene name);
UBA3 (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
[NEDD8 protein]:[E1 NEDD8-activating enzyme] ligase (AMP-forming)
Reaction(IUBMB)
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
Substrate
ATP [CPD:
C00002 ];
[NEDD8 protein];
[E1 NEDD8-activating enzyme]-L-cysteine
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
Comment
Some RING-type E3 ubiquitin transferase (EC
2.3.2.27 ) are not able to bind a substrate protein directly. Instead, they form complexes with a cullin scaffold protein and a substrate recognition module, which are known as CRL (Cullin-RING-Ligase) complexes. The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation. Like ubiquitin, the NEDD8 protein ends with two glycine residues. The E1 NEDD8-activating enzyme activates NEDD8 in an ATP-dependent reaction by forming a high-energy thioester intermediate between NEDD8 and one of its cysteine residues. The activated NEDD8 is subsequently transferred to a cysteine residue of EC
2.3.2.34 , E2 NEDD8-conjugating enzyme, and is eventually conjugated to a lysine residue of specific substrates in the presence of the appropriate E3 transferase (EC
2.3.2.32 , cullin-RING-type E3 NEDD8 transferase).
History
EC 6.2.1.64 created 2020
Orthology
K10686 NEDD8-activating enzyme E1
Genes
CIMI : 108283730 108314065(UBA3)
NVS : 122898856 122899207 122899214 122909279(UBA3) 122913945
TOD : 119230920(UBA3) 119244539
ASAO : 132769411(UBA3) 132775615
XLA : 108714725(uba3.L) 734782(uba3.S)
SRX : 107729399 107737645 107753775
SANH : 107656056 107672020 107696817
SGH : 107558885 107580149 107600288
CCAR : 109061212 109061224 109077613
CAUA : 113055001 113069100 113111027
CGIB : 127968206 128012157 128022425
PTET : 122329179 122354265(uba3)
LROH : 127155243 127173545(uba3)
OMC : 131532426 131549013(uba3)
MASI : 127426278 127428228
MANU : 129427649 129427651(uba3)
CMAO : 118799796(uba3) 118822616
CHAR : 105906787 105912256(uba3)
NCC : 104944983 104955224(uba3)
TBEN : 117475652(uba3) 117479473
CGOB : 115008263 115011383
PGEO : 117446651(uba3) 117450116
GACU : 117543134 117543692(uba3)
EMAC : 134866439 134883547(uba3)
ELY : 117256264(uba3) 117258904
EFO : 125891409 125893796(uba3)
PLEP : 121946477 121949671(uba3)
SLUC : 116035189(uba3) 116061992
ECRA : 117943434(uba3) 117947055
PFLV : 114554110 114558205
GAT : 120828986 120835094(uba3)
PPUG : 119194095(uba3) 119222001
AFB : 129099610 129105657(uba3)
CLUM : 117731384(uba3) 117733286
PSWI : 130199322 130207973(uba3)
MSAM : 119886654(uba3) 119906123
SCHU : 122865348(uba3) 122883402
CUD : 121506998(uba3) 121516911
ALAT : 119021901(uba3) 119022861
OAU : 116310945 116330178(uba3)
OML : 112145215(uba3) 112158859
XMA : 102225974 102230113(uba3)
PRET : 103465053 103467665
PFOR : 103144012 103152910
PLAI : 106934653 106953339
PMEI : 106903451 106907052
GAF : 122832545 122834364(uba3)
PPRL : 129358396(uba3) 129370412
CTUL : 119782023 119789537
GMU : 124856575(uba3) 124870880
NFU : 107385429(uba3) 107396417
KMR : 108231938 108241173(uba3)
ALIM : 106518515(uba3) 106519301
NWH : 119411712(uba3) 119416830
AOCE : 111572109 111588779
MCEP : 125005552 125007431(uba3)
SSEN : 122768622 122777873
HHIP : 117761467(uba3) 117764866
HSP : 118105365(uba3) 118111501
SMAU : 118309923(uba3) 118317018
SLAL : 111655022 111670887
XGL : 120783499(uba3) 120803892
MALB : 109955907 109966755
BSPL : 114856283(uba3) 114859396
SJO : 128355467(uba3) 128357016
SASA : 100196693(uba3) 106567275(uba3) 106572411
STRU : 115150783 115168117(uba3)
OGO : 124031431 124032034 124045894
OKE : 118359856 118360401 118400157
SALP : 111966608 111969932 111976991
SNH : 120058867 120061175 120065095
CCLU : 121538490 121545638 121578473
SFM : 108923369(uba3) 108940090
PSPA : 121297887 121329119
ARUT : 117411901 117413772
DSI : Dsimw501_GD10991(Dsim_GD10991)
CLON : 129610295 129617884
CQU : CpipJ_CPIJ015388 CpipJ_CPIJ016052
SGRE : 126293670 126318312
IEL : 124154708 124155380 124171480
HAZT : 108673009 108677282
PPOI : 119107499 119108610
CBR : CBG_16503(Cbr-rfl-1)
BMY : BM_BM3316(Bma-rfl-1)
MMER : 123530526 128547822
CSAT : 104706240 104735980 104770273
BNA : 106386769 106433658 106434242 111206274
HSYR : 120175254 120191703
LJA : Lj4g3v2400830.1(Lj4g3v2400830.1)
LANG : 109329614 109329616 109337793
QSA : O6P43_003417 O6P43_021610
MSYL : 126596448 126608648
MESC : 110601552 110611230
PALZ : 118029704 118061045 118062744
SSPL : 121783630 121797418 121799575 121808475
SHIS : 125197539 125223467
AEW : 130763986 130764804 130775061
PSOM : 113336889 113357346
DOSA : Os01t0271500-01(Os01g0271500)
TAES : 123061091 123069678 123078164
TUA : 125543715 125543716 125543717
PVIR : 120673559 120706510
SMO : SELMODRAFT_106904 SELMODRAFT_174709
MPP : MICPUCDRAFT_3065(JGI:MicpuC2_3065)
NCS : NCAS_0A11140(NCAS0A11140)
NDI : NDAI_0A05160(NDAI0A05160)
TPF : TPHA_0A03600(TPHA0A03600)
TBL : TBLA_0H01590(TBLA0H01590)
TDL : TDEL_0C04980(TDEL0C04980)
KAF : KAFR_0H00930(KAFR0H00930)
KNG : KNAG_0H00670(KNAG0H00670)
CAL : CAALFM_C600500CA(CaO19.4209)
NTE : NEUTE1DRAFT133618(NEUTE1DRAFT_133618)
CCAC : CcaHIS019_0200870(uba3)
ABP : AGABI1DRAFT111125(AGABI1DRAFT_111125)
ABV : AGABI2DRAFT190343(AGABI2DRAFT_190343)
SCM : SCHCO_02607608(SCHCODRAFT_02607608)
PGR : PGTG_00608 PGTG_17926 PGTG_20639
PSTR : Pst134EA_000626 Pst134EA_030431
EHI : EHI_098550(19.t00019)
PTI : PHATRDRAFT_33774(UBA3)
EHX : EMIHUDRAFT_425916 EMIHUDRAFT_428127
TVA : TVAG_2v0228020 TVAG_2v0330060
LOKI : Lokiarch_29320(moeB_2)
» show all
Taxonomy
Reference
Authors
Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S
Title
A new NEDD8-ligating system for cullin-4A.
Journal
Sequence
Reference
Authors
Gong L, Yeh ET
Title
Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.64
ExPASy - ENZYME nomenclature database: 6.2.1.64