Entry
Name
2,3-dihydroxybenzoate---[aryl-carrier protein] ligase;
entE (gene name);
vibE (gene name);
dhbE (gene name);
angE (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
2,3-dihydroxybenzoate:[aryl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + 2,3-dihydroxybenzoate + holo-[aryl-carrier protein] = AMP + diphosphate + 2,3-dihydroxybenzoyl-[aryl-carrier protein] (overall reaction) [RN:
R12777 ];
(1a) ATP + 2,3-dihydroxybenzoate = diphosphate + (2,3-dihydroxybenzoyl)adenylate [RN:
R01504 ];
(1b) (2,3-dihydroxybenzoyl)adenylate + holo-[aryl-carrier protein] = AMP + 2,3-dihydroxybenzoyl-[aryl-carrier protein] [RN:
R12776 ]
Reaction(KEGG)
Substrate
ATP [CPD:
C00002 ];
2,3-dihydroxybenzoate [CPD:
C00196 ];
holo-[aryl-carrier protein] [CPD:
C20665 ];
(2,3-dihydroxybenzoyl)adenylate [CPD:
C04030 ]
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
2,3-dihydroxybenzoyl-[aryl-carrier protein] [CPD:
C22408 ];
(2,3-dihydroxybenzoyl)adenylate [CPD:
C04030 ]
Comment
The adenylation domain of the enzyme catalyses the activation of 2,3-dihydroxybenzoate to (2,3-dihydroxybenzoyl)adenylate, followed by the transfer the activated compound to the free thiol of a phosphopantetheine arm of an aryl-carrier protein domain of a specific non-ribosomal peptide synthase. For example, the EntE enzyme of Escherichia coli is part of the enterobactin synthase complex, the VibE enzyme of Vibrio cholerae is part of the vibriobactin synthase complex, and the DhbE enzyme of Bacillus subtilis is part of the bacillibactin synthase complex.
History
EC 6.2.1.71 created 2021 (EC 2.7.7.58 created 1992, incorporated 2021)
Pathway
ec01053 Biosynthesis of siderophore group nonribosomal peptides
Orthology
K02363 2,3-dihydroxybenzoate---[aryl-carrier protein] ligase
Genes
ECD : ECDH10B_0554(entE) ECDH10B_0662(entE)
ECOO : ECRM13514_0616(entE)
ECOH : ECRM13516_0560(entE)
ELR : ECO55CA74_03710(entE)
ECW : EcE24377A_0614(entE)
ECOA : APECO78_06550(entE)
EDJ : ECDH1ME8569_0564(entE)
ECOI : ECOPMV1_00612(dhbE_1)
SEM : STMDT12_C06580(entE)
SETC : CFSAN001921_14055(entE)
SEEN : SE451236_09000(entE)
SENH : CFSAN002069_05865(entE)
SEEH : SEEH1578_12410(entE)
SENJ : CFSAN001992_08350(entE)
SEEC : CFSAN002050_09590(entE)
SEEB : SEEB0189_016300(entE)
SENC : SEET0819_09945(entE)
REE : electrica_03706(entE)
KIE : NCTC12125_02371(dhbE_2)
AHN : NCTC12129_03600(entE)
METY : MRY16398_41480(entE)
SMW : SMWW4_v1c03160(entE)
SERS : SERRSCBI_01440(entE)
SFJ : SAMEA4384070_0306(dhbE)
SOF : NCTC11214_03248(dhbE)
SERA : Ser39006_010830(entE)
DIC : Dpoa569_001066(entE)
DDA : Dd703_1489 Dd703_3074
TPTY : NCTC11468_00301(dhbE)
LAG : N175_10460(entE) N175_19255(entE)
VAU : VANGNB10_67p012c(angE) VANGNB10_cI0894c(vabE)
VPL : SA104470976_01956(dhbE)
PFW : PF1751_v1c25710(pchD)
PSED : DM292_17525 DM292_17580
PLZ : S4054249_19780(entE)
AEL : NCTC12917_01758(entE)
LMIR : NCTC12852_01587(dhbE)
CDN : BN940_00281 BN940_03671
HYN : F9K07_05605 F9K07_23795
RPUS : CFBP5875_15640(entE)
BVY : NCTC9239_02600(dhbE)
DTI : Desti_0832 Desti_0842
MFB : MFUL124B02_23120(entE)
SCU : SCE1572_23325(entE) SCE1572_43370
BAZ : BAMTA208_15890(dhbE)
BSON : S101395_00489(entE)
PARG : PspKH34_01740(dhbE)
CPRE : Csp1_05900(dhbE_1) Csp1_26980(dhbE_2)
CCYS : SAMEA4530656_2121(entE2)
NBR : O3I_007905 O3I_033030
NTP : CRH09_08850 CRH09_09670
NAD : NCTC11293_01882(dhbE_2)
NIE : KV110_22505 KV110_22835 KV110_32010 KV110_35825 KV110_35895
NHU : H0264_13975 H0264_16235
RRT : 4535765_00739(dhbE_1)
RCR : NCTC10994_01991(dhbE)
GPD : GII33_06980 GII33_11340
TPUL : TPB0596_40190(entE)
SANL : KZO11_01055 KZO11_19365
SSX : SACTE_0742 SACTE_1756 SACTE_6073
SGU : SGLAU_04525 SGLAU_04655(dhbE)
STRE : GZL_08593 GZL_p00203
SLE : sle_05720(sle_05720) sle_35590(sle_35590)
STRT : A8713_24055 A8713_24150 A8713_28985
SGM : GCM10017557_45820(dhbE)
SPHW : NFX46_15510 NFX46_19195
SNR : SNOUR_28950 SNOUR_38895
SKA : CP970_00590 CP970_25100
SPAD : DVK44_09655 DVK44_32335
SRIM : CP984_09365 CP984_39415
SHUN : DWB77_06837(dhbE_1) DWB77_07040(dhbE_2)
SXN : IAG42_33255 IAG42_36260
SDUR : M4V62_01695 M4V62_08460
STUB : MMF93_02530 MMF93_23480
SXT : KPP03845_101124(dhbE)
KAB : B7C62_00860 B7C62_28990
NAEI : GCM126_00280(pchD_1) GCM126_36390(pchD_2)
TALX : FOF52_04695 FOF52_08965
NEX : NE857_12340 NE857_18255
NCG : KGD84_12920 KGD84_20115
AGRA : AGRA3207_003654 AGRA3207_007732
FRE : Franean1_3057 Franean1_3615
AJA : AJAP_21440(dhbE1) AJAP_35485(dhbE2)
AORI : SD37_07355 SD37_10185
AROO : NQK81_04815 NQK81_41805
PSEE : FRP1_00460(entE) FRP1_17760(entE)
PSEQ : AD006_08100(entE) AD006_25210(entE)
PECQ : AD017_04800(entE) AD017_15935(entE)
PHH : AFB00_13770(entE) AFB00_17115 AFB00_19590
PAUT : Pdca_37510(dhbE_1) Pdca_45210(dhbE_2) Pdca_61910(entE)
SSYI : EKG83_25955 EKG83_32800
MCRA : ID554_15095 ID554_18280 ID554_21220 ID554_27900
ACTL : L3i22_035590(dhbE) L3i22_066090(dhbE) L3i22_072050(mbtA)
DFU : Dfulv_14540 Dfulv_14620 Dfulv_14765
BALA : DSM104299_03973(dhbE)
» show all
Taxonomy
Reference
Authors
Gehring AM, Bradley KA, Walsh CT.
Title
Enterobactin biosynthesis in Escherichia coli: isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate.
Journal
Reference
Authors
Wyckoff EE, Stoebner JA, Reed KE, Payne SM.
Title
Cloning of a Vibrio cholerae vibriobactin gene cluster: identification of genes required for early steps in siderophore biosynthesis.
Journal
Sequence
Reference
Authors
Ehmann DE, Shaw-Reid CA, Losey HC, Walsh CT.
Title
The EntF and EntE adenylation domains of Escherichia coli enterobactin synthetase: sequestration and selectivity in acyl-AMP transfers to thiolation domain cosubstrates.
Journal
Reference
Authors
Keating TA, Marshall CG, Walsh CT.
Title
Vibriobactin biosynthesis in Vibrio cholerae: VibH is an amide synthase homologous to nonribosomal peptide synthetase condensation domains.
Journal
Sequence
Reference
Authors
May JJ, Wendrich TM, Marahiel MA
Title
The dhb operon of Bacillus subtilis encodes the biosynthetic template for the catecholic siderophore 2,3-dihydroxybenzoate-glycine-threonine trimeric ester bacillibactin.
Journal
Reference
Authors
Sikora AL, Wilson DJ, Aldrich CC, Blanchard JS.
Title
Kinetic and inhibition studies of dihydroxybenzoate-AMP ligase from Escherichia coli.
Journal
Reference
Authors
Khalil S, Pawelek PD
Title
Enzymatic adenylation of 2,3-dihydroxybenzoate is enhanced by a protein-protein interaction between Escherichia coli 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (EntA) and 2,3-dihydroxybenzoate-AMP ligase (EntE).
Journal
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.71
ExPASy - ENZYME nomenclature database: 6.2.1.71