Entry
Name
L-serine---[L-seryl-carrier protein] ligase;
entF (gene name);
zmaJ (gene name);
gdnB (gene name);
serine-activating enzyme
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
L-serine:[L-seryl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-serine + holo-[L-seryl-carrier protein] = AMP + diphosphate + L-seryl-[L-seryl-carrier protein] (overall reaction);
(1a) ATP + L-serine = diphosphate + (L-seryl)adenylate [RN:
R12779 ];
(1b) (L-seryl)adenylate + holo-[L-seryl-carrier protein] = AMP + L-seryl-[L-seryl-carrier protein]
Reaction(KEGG)
Substrate
ATP [CPD:
C00002 ];
L-serine [CPD:
C00065 ];
holo-[L-seryl-carrier protein];
(L-seryl)adenylate [CPD:
C05820 ]
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
L-seryl-[L-seryl-carrier protein];
(L-seryl)adenylate [CPD:
C05820 ]
Comment
The adenylation domain of the enzyme catalyses the activation of L-serine to (L-seryl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of a peptidyl-carrier protein domain. The peptidyl-carrier protein domain may be part of the same protein, or of a different protein. This activity is often found as part of a larger non-ribosomal peptide synthase.
History
EC 6.2.1.72 created 2021
Pathway
ec01053 Biosynthesis of siderophore group nonribosomal peptides
Orthology
K02364 L-serine---[L-seryl-carrier protein] ligase
Genes
ECD : ECDH10B_0546(entF) ECDH10B_0654(entF)
ECOO : ECRM13514_0608(entF)
ECOH : ECRM13516_0552(entF)
ELR : ECO55CA74_03670(entF)
ECW : EcE24377A_0606(entF)
ECOA : APECO78_06510(entF)
EDJ : ECDH1ME8569_0556(entF)
ECOI : ECOPMV1_00604(entF)
SEM : STMDT12_C06500(entF)
SETC : CFSAN001921_14095(entF)
SEEN : SE451236_08960(entF)
SENH : CFSAN002069_05905(entF)
SEEH : SEEH1578_12370(entF)
SEEC : CFSAN002050_09550(entF)
SEEB : SEEB0189_016340(entF)
SENC : SEET0819_09905(entF)
REE : electrica_03713(entF)
RTG : NCTC13098_05440(entF_1) NCTC13098_05441(entF_2)
KIE : NCTC12125_02379(entF_1) NCTC12125_02380(entF_2)
AHN : NCTC12129_03608(entF_1)
METY : MRY16398_41550(entF)
SMAR : SM39_4660 SM39_4670(entF)
SMAC : SMDB11_4402 SMDB11_4411(entF)
SMW : SMWW4_v1c03040 SMWW4_v1c03130
SRR : SerAS9_0899 SerAS9_3620
SRL : SOD_c08290(dhbF1) SOD_c33480(entF)
SRY : M621_04560 M621_18205(entF)
SPLY : Q5A_004410(dhbF_1) Q5A_018140(entF)
SRS : SerAS12_0899 SerAS12_3621
SRA : SerAS13_0899 SerAS13_3622
SLQ : M495_04150 M495_17660(entF)
SERF : L085_02490(entF) L085_02540 L085_10400(entF)
SERS : SERRSCBI_01380 SERRSCBI_01425(entF)
SFW : WN53_13375 WN53_26515(entF)
SFG : AV650_08925 AV650_22600(entF)
SQU : E4343_11595 E4343_23565
SFJ : SAMEA4384070_0294(dhbF_1) SAMEA4384070_0303(entF)
SSUR : ATE40_022265 ATE40_022310(entF)
SURI : J0X03_05725 J0X03_22420 J0X03_22465
SENP : KHA73_01245 KHA73_01290
SNEM : NLX84_01310 NLX84_01355
SNEV : OI978_04965 OI978_05010
GQU : AWC35_14270 AWC35_18935(entF)
TPTY : NCTC11468_00309(entF)
XFU : XFF4834R_chr38100 XFF4834R_chr38110
XPH : XppCFBP6546_14790(XppCFBP6546P_14790)
XHD : LMG31886_40570(dhbF)
VPL : SA104470976_01311(dltA)
ZPL : ZBT109_1264 ZBT109_2392 ZBT109_2393
LMIR : NCTC12852_01585(dhbF)
AVV : RvVAT039_06970(entF)
AVF : RvVAR031_26920(entF)
BVL : BF3285c2_0781(entF1)
BIO : BR141012304_20996(entF)
BVY : NCTC9239_02597(dhbF)
SPSE : SULPSESMR1_03056(dhbF)
PYE : A6J80_08735 A6J80_11740
PAMN : JCM7685_0408(entF1) JCM7685_2850(entF3)
PKD : F8A10_00900 F8A10_09700
PPAN : ESD82_11360 ESD82_19250
PSEW : JHW44_08185 JHW44_17535
PVER : E3U25_17940 E3U25_21275
PFEO : E3U26_06405 E3U26_09545
CCYS : SAMEA4530656_2119(nrpS4)
NAD : NCTC11293_06435(entF)
NWL : NWFMUON74_31280(entF)
STRT : A8713_24165 A8713_28970
SQZ : FQU76_00195 FQU76_33545
SRIM : CP984_01955 CP984_39855
STRZ : OYE22_21905 OYE22_28035
ACTU : Actkin_04386(dhbF_4)
BALA : DSM104299_03971(dhbF)
» show all
Taxonomy
Reference
Authors
Pettis GS, McIntosh MA
Title
Molecular characterization of the Escherichia coli enterobactin cistron entF and coupled expression of entF and the fes gene.
Journal
Reference
Authors
Rusnak F, Sakaitani M, Drueckhammer D, Reichert J, Walsh CT
Title
Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheine.
Journal
Sequence
Reference
Authors
Reichert J, Sakaitani M, Walsh CT.
Title
Characterization of EntF as a serine-activating enzyme.
Journal
Reference
Authors
Ehmann DE, Shaw-Reid CA, Losey HC, Walsh CT.
Title
The EntF and EntE adenylation domains of Escherichia coli enterobactin synthetase: sequestration and selectivity in acyl-AMP transfers to thiolation domain cosubstrates.
Journal
Reference
Authors
Chan YA, Boyne MT 2nd, Podevels AM, Klimowicz AK, Handelsman J, Kelleher NL, Thomas MG.
Title
Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units.
Journal
Reference
Authors
Frueh DP, Arthanari H, Koglin A, Vosburg DA, Bennett AE, Walsh CT, Wagner G.
Title
Dynamic thiolation-thioesterase structure of a non-ribosomal peptide synthetase.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.72
ExPASy - ENZYME nomenclature database: 6.2.1.72