KEGG ENZYME: 6.3.1.13

ENZYME: 6.3.1.13

Entry

EC 6.3.1.13                 Enzyme

Name

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase;
MshC;
MshC ligase;
Cys:GlcN-Ins ligase;
mycothiol ligase

Class

Ligases;
Forming carbon-nitrogen bonds;
Acid-D-ammonia (or amine) ligases (amide synthases)

Sysname

L-cysteine:1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol ligase (AMP-forming)

Reaction(IUBMB)

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate [RN:R09591]

Reaction(KEGG)

R09591

Substrate

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol [CPD:C19702];
L-cysteine [CPD:C00097];
ATP [CPD:C00002]

Product

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol [CPD:C19703];
AMP [CPD:C00020];
diphosphate [CPD:C00013]

Comment

This enzyme is a key enzyme in the biosynthesis of mycothiol, a small molecular weight thiol found in Mycobacteria spp. and other actinomycetes. Mycothiol plays a fundamental role in these organisms by helping to provide protection from the effects of reactive oxygen species and electrophiles, including many antibiotics. The enzyme may represent a novel target for new classes of antituberculars [2].

History

EC 6.3.1.13 created 2009

Orthology

K15526

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Genes

ERZ:	ER308_04690

EUZ:	DVS28_a1275

MTU:	Rv2130c(mshC)

MTV:	RVBD_2130c

MTC:	MT2188(cysS-1)

MRA:	MRA_2144(cysS1)

MTF:	TBFG_12161

MTB:	TBMG_01851

MTK:	TBSG_01863

MTZ:	TBXG_001834

MTG:	MRGA327_13125

MTI:	MRGA423_13240

MTE:	CCDC5079_1969

MTUR:

CFBS_2257(cysS1)

MTL:	CCDC5180_1943

MTO:	MTCTRI2_2165(cysS)

MTD:	UDA_2130c(cysS2)

MTN:	ERDMAN_2347(cysS)

MTJ:	J112_11410

MTUB:

MT7199_2160(mshC)

MTUC:

J113_14765

MTUE:

J114_11415

MTX:	M943_11010

MTUL:

TBHG_02085

MTUT:

HKBT1_2248(cysS1)

MTUU:

HKBT2_2250(cysS1)

MTQ:	HKBS1_2254(cysS1)

MBO:	BQ2027_MB2154C(mshc)

MBB:	BCG_2147c(cysS2)

MBT:	JTY_2141(cysS2)

MBM:	BCGMEX_2133c(cysS2)

MBK:	K60_022110

MBX:

BCGT_1951

MAF:	MAF_21420(cysS2)

MMIC:

RN08_2361

MCE:	MCAN_21541(cysS2)

MCQ:	BN44_50059(cysS)

MCV:	BN43_31347(cysS)

MCX:	BN42_40042(cysS)

MCZ:	BN45_50451(cysS)

MORY:

MO_002230(mshC)

MLE:	ML1302(cysS2)

MLB:	MLBr01302(cysS2)

MPA:	MAP_1876c(cysS2)

MAO:

MAP4_1953

MAVI:

RC58_09755

MAVU:

RE97_09760

MAV:	MAV_2361(cysS)

MIT:

OCO_23640

MIA:

OCU_23520

MID:

MIP_03257

MYO:

OEM_22060

MCHI:

AN480_11905

MIR:

OCQ_22160

MMAL:

CKJ54_10685

MLP:

MLM_2236

MMAN:

MMAN_16200(mshC)

MSA:	Mycsm_03809

MUL:	MUL_2360(mshC)

MMI:	MMAR_3110(mshC)

MMAE:

MMARE11_30320(mshC)

MLI:	MULP_03371(mshC)

MPSE:

MPSD_32180(mshC)

MSHO:

MSHO_45680(mshC)

MMC:

Mmcs_3153

MKM:

Mkms_3215

MJL:

Mjls_3165

MMM:

W7S_11410

MKN:	MKAN_02345

MYV:	G155_17845

MYE:	AB431_18405

MHAD:

B586_10540

MDX:	BTO20_13850

MSHG:

MSG_03006(mshC)

MFJ:	MFLOJ_02080(mshC)

MSTO:

MSTO_02740(mshC)

MSIM:

MSIM_46870(mshC)

MSAK:

MSAS_10650(mshC)

MKU:	I2456_15270

MLW:	MJO58_16090(mshC)

MXE:	MYXE_22970(mshC)

MNV:	MNVI_43580(mshC)

MPAG:

C0J29_17655

MNM:	MNVM_00970(mshC)

MGOR:

H0P51_16610

MCOO:

MCOO_11380(mshC)

MBAI:

MB901379_02881(mshC)

MSEO:

MSEO_18160(mshC)

MHEK:

JMUB5695_02230(mshC)

MGAU:

MGALJ_14100(mshC)

MLJ:	MLAC_21310(mshC)

MBRD:

MBRA_36880(mshC)

MSHJ:

MSHI_11390(mshC)

MMAM:

K3U93_13990(mshC)

MSPG:

F6B93_13290

MOT:	LTS72_08815(mshC)

MLM:	MLPF_1846(mshC)

MPAA:

MKK62_11015(mshC)

MMEH:

M5I08_13140(mshC)

MKY:	IWGMT90018_26740(mshC)

MADI:

A7U43_16825

MWU:	PT015_06785(mshC)

MSM:	MSMEG_4189(cysS)

MSG:	MSMEI_4091(cysS2)

MSB:	LJ00_20770

MSN:	LI99_20775

MSH:	LI98_20780

MVA:

Mvan_3478

MGI:

Mflv_3049

MSP:	Mspyr1_23640

MCB:	Mycch_3131

MNE:	D174_14110

MYN:	MyAD_13865

MFT:	XA26_36370(mshC)

MPHL:

MPHLCCUG_03253(mshC)

MVQ:	MYVA_3402(cysS1)

MLL:	B1R94_11530

MRH:	MycrhN_5129

MTHN:

4412656_01910(mshC)

MHAS:

MHAS_01577(mshC)

MDU:	MDUV_09380(mshC)

MCHT:

MCHIJ_34980(mshC)

MDR:	MDOR_23330(mshC)

MAUU:

NCTC10437_03369(mshC)

MMAG:

MMAD_33800(mshC)

MMOR:

MMOR_36240(mshC)

MFX:	MFAL_11990(mshC)

MAIC:

MAIC_32490(mshC)

MIJ:	MINS_30510(mshC)

MALV:

MALV_03630(mshC)

MTY:	MTOK_53970(mshC)

MPSC:

MPSYJ_32140(mshC)

MARZ:

MARA_05810(mshC)

MGAD:

MGAD_10700(mshC)

MHEV:

MHEL_57800(mshC)

MSAR:

MSAR_37340(mshC)

MANY:

MANY_17490(mshC)

MAUB:

MAUB_52910(mshC)

MPOF:

MPOR_36750(mshC)

MPHU:

MPHO_16650(mshC)

MMUC:

C1S78_010120

MMAT:

MMAGJ_71410(mshC)

MBOK:

MBOE_37710(mshC)

MFG:	K6L26_14120(mshC)

MSEI:

MSEDJ_45890(mshC)

MFLV:

NCTC10271_02066(mshC)

MCEE:

MCEL_04990(mshC)

MBRM:

L2Z93_001534(mshC)

MMON:

EWR22_16645

MGO:	AFA91_19155

MHOL:

K3U96_11385(mshC)

MSEN:

K3U95_15230(mshC)

MPAE:

K0O64_17755(mshC)

MRF:	MJO55_15960(mshC)

MDF:	K0O62_14330(mshC)

MCRO:

MI149_18595(mshC)

MGRO:

FZ046_21870

MKR:	MKOR_06390(mshC)

MPAK:

MIU77_07475(mshC)

MAB:

MAB_2116

MMV:	MYCMA_1106

MABB:

MASS_2039

MABL:

MMASJCM_2065

MCHE:

BB28_10590

MIZ:	BAB75_11340

MSTE:

MSTE_02046(mshC)

MSAO:

MYCSP_08980

MSAL:

DSM43276_01828(mshC)

MJD:	JDM601_1772(mshC)

MTER:

4434518_01718(mshC)

MMIN:

MMIN_33980(mshC)

MHIB:

MHIB_12110(mshC)

MHER:

K3U94_10025(mshC)

MVM:	MJO54_10115(mshC)

ASD:	AS9A_1960(mshC)

CGL:	Cgl1514(Cgl1514)

CGB:	cg1709(cysS2)

CGU:	WA5_1457(CysS)

CGT:

cgR_1574

CGS:	C624_08375

CGG:	C629_08385

CGM:	cgp_1709(mshC)

CGJ:

AR0_08110

CGQ:	CGLAR1_07975

CGX:	SB89_07810

CEF:	CE1639(cysS)

CDI:	DIP1261(cysS2)

CDP:	CD241_1191(cysS2)

CDH:	CDB402_1170(cysS2)

CDT:	CDHC01_1190(cysS2)

CDE:	CDHC02_1169(cysS2)

CDR:	CDHC03_1164(cysS2)

CDA:	CDHC04_1174(cysS2)

CDZ:	CD31A_1272(cysS2)

CDB:	CDBH8_1239(cysS2)

CDS:	CDC7B_1255(cysS2)

CDD:	CDCE8392_1162(cysS2)

CDW:	CDPW8_1240(cysS2)

CDV:	CDVA01_1131(cysS2)

CDIP:

ERS451417_01261(cysS2)

CJK:	jk0368(cysS2) jk0956(cysS1)

CUR:	cu0281 cu1032

CUA:	CU7111_1014(mshC)

CAR:	cauri_1250(mshC)

CKP:	ckrop_0920(mshC)

CPU:	CPFRC_05295(mshC)

CPL:	Cp3995_1074(mshC)

CPG:	CP316_05445(mshC)

CPP:	CpP54B96_1068(mshC)

CPK:	CP1002_07850(mshC)

CPQ:	CPC231_05300(mshC)

CPX:	CPI19_06220(mshC)

CPZ:	CpPAT10_1048(mshC)

COR:	Cp267_1100(mshC)

COP:	CP31_05675(mshC)

COD:	Cp106_1031(mshC)

COS:	Cp4202_1041(mshC)

COI:	CPCIP5297_05465(mshC)

COE:	CP258_05460(mshC)

COU:	CP162_05830(mshC)

CPSE:

CPTA_01622

CPSU:

CPTB_02141

CPSF:

CPTC_01613

CRD:	CRES_1038(mshC)

CUL:	CULC22_01124(mshC)

CUC:	CULC809_01109(mshC)

CUE:	CULC0102_1233(cysS)

CUN:	Cul210932_1165(mshC)

CUQ:	Cul210931_1115(mshC)

CUZ:	Cul05146_1189(mshC)

CUJ:	CUL131002_1147c(mshC)

CUS:	CulFRC11_1133(mshC)

CVA:	CVAR_1445(mshC)

CHN:	A605_07425

CCN:	H924_06935

CTER:

A606_05610

CMD:	B841_06875

CAZ:	CARG_04870

CFN:	CFAL_05375

CCG:	CCASEI_07275

CVT:	B843_07135

CGY:	CGLY_08655(mshC2)

CAX:	CATYP_05200

CII:	CIMIT_06450

CUV:	CUREI_06185

COA:	DR71_50(mshC)

CDO:	CDOO_07385

CHM:	B842_06785

CSX:	CSING_06315 CSING_06890(mshC)

CMQ:	B840_06340(mshC)

CKU:	UL82_05260(mshC)

CCJ:	UL81_06070(mshC)

CMV:	CMUST_08100(mshC)

CEI:	CEPID_06495(mshC)

CTED:

CTEST_06690(mshC)

CUT:	CUTER_05510(mshC)

CLW:	CLAC_06420

CDX:	CDES_07295(mshC)

CSP:	WM42_0105(mshC)

CSTA:

CSTAT_06590

CCJZ:

ccrud_07370

CFK:	CFRA_05760

CPHO:

CPHO_06025

CFC:	CFLV_06410

CGV:	CGLAU_06465(mshC)

CSTR:

CBE89_07260

CAQU:

CAQU_06410

CSPH:

CSPHI_06640

CAMG:

CAMM_06785

CMIN:

NCTC10288_01274(mshC)

CPEG:

CPELA_05510(mshC)

CXE:	FOB82_12335(mshC)

CEE:	CENDO_05835(mshC)

CSAN:

E3227_09300(mshC)

CGK:	CGERO_05625(mshC)

CRF:	FRC0190_01236

CRL:	NCTC7448_01926(CysS)

CCHO:

CCHOA_05890(mshC)

CPRE:

Csp1_14170(mshC)

CPSO:

CPPEL_05710(mshC)

CSUR:

N24_1606(cysS)

CCYS:

SAMEA4530656_1085(cysS2)

CMAT:

HBA49_00360(mshC)

CYZ:	C3B44_05605(mshC)

CKF:	I6I12_06655(mshC)

CANS:

GP473_04330(mshC)

CLIA:

C3E79_05615(mshC)

CPRP:

I6I69_11000(mshC)

CAMC:

I6I65_02650(mshC)

CGLU:

I6J20_03620(mshC) I6J20_08460(mshC)

CUO:	CUROG_04860(mshC)

CLIZ:

G7Y31_05915(mshC)

CSIL:

CBE74_06320

CTUB:

I6I74_11155(mshC)

CLUJ:

IAU68_05995(mshC)

CQN:	G7Y29_05490(mshC)

CKW:	CKALI_05760(mshC)

CCOE:

CETAM_06765(mshC)

COK:	COCCU_07455(mshC)

CZO:	IAU67_04340(mshC)

CCYC:

SCMU_20620(mshC)

CORY:

FQV43_05160(mshC)

CMF:	GWO64_006130(mshC)

CWK:	IA203_06590(mshC)

CUW:	LH390_05870(mshC)

CPOY:

GP475_05960(mshC)

CACC:

CACC_06460(mshC)

CFY:	I6L56_02795(mshC)

CPSL:

KBP54_05900(mshC)

CHIN:

J5O04_08140(mshC)

CJH:	CJEDD_06595(mshC)

CPSP:

L9H27_05330(mshC)

CMAS:

CMASS_05400(mshC)

CHEI:

CHEID_04965(mshC)

CIHU:

CIHUM_05760(mshC)

CCOY:

CCOY_06500(mshC)

CHAD:

CHAD_06320(mshC3)

CFG:	CFREI_06860(mshC)

CAQM:

CAQUA_05715(mshC)

CCAW:

CCANI_07230(mshC)

CAUI:

CAURIS_05780(mshC)

CFAC:

CFAEC_07285(mshC)

CFOU:

CFOUR_05840(mshC)

CFEU:

CFELI_07035(mshC)

CAUS:

CAURIC_05255(mshC)

CATR:

CATRI_06620(mshC)

CDUR:

CDUR_06590(mshC)

CCOU:

CCONF_06080(mshC)

CHAN:

CHAN_07040(mshC)

CAFE:

CAFEL_05705(mshC)

CGF:	CGUA_06735(mshC)

CGOI:

CGOTT_06020(mshC)

CAPP:

CAPP_06065(mshC)

CBOV:

CBOVI_05675(mshC)

CSUE:

QP029_11040(mshC)

CBRE:

QP027_05580(mshC)

CPYR:

CYJ47_06035(mshC) CYJ47_10975(mshC)

CRIE:

AK829_03405

BFV:	C628_08310

NFA:	NFA_29340(mshC)

NFR:	ERS450000_01160(mshC)

NCY:	NOCYR_2775(cysS)

NBR:	O3I_020965

NNO:	NONO_c37250(mshC)

NSL:	BOX37_16095

NSR:	NS506_04748(mshC)

NTP:	CRH09_19110(mshC)

NOZ:	DMB37_01595(mshC)

NOD:	FOH10_12980(mshC)

NAH:	F5544_22350(mshC)

NAD:	NCTC11293_04018(mshC)

NWL:	NWFMUON74_33090(mshC)

NIE:	KV110_21730(mshC)

NTC:	KHQ06_23545(mshC)

NHU:	H0264_23835(mshC)

NYA:	LTV02_11300(mshC)

NGP:	LTT66_33500(mshC)

NSPU:

IFM12276_32640(mshC)

NYU:	D7D52_20030(mshC)

RHA:	RHA1_ro00877

RER:	RER_31960(mshC)

REY:

O5Y_14690

REB:	XU06_14915

RQI:	C1M55_15935(mshC)

ROP:	ROP_06130(mshC)

ROA:	Pd630_LPD04960

RPY:	Y013_02985

RBY:	CEJ39_13660(mshC)

RHB:

NY08_1030

RAV:	AAT18_13580

RFA:	A3L23_04280(mshC)

RHS:	A3Q41_03986(mshC)

RHW:	BFN03_08490

RRZ:	CS378_03350(mshC)

RHU:	A3Q40_01708(mshC)

RHQ:	IM25_16935

RHOD:

AOT96_26125

RRT:	4535765_02337(mshC)

RCR:	NCTC10994_00506(mshC)

RTM:	G4H71_21950(mshC)

RKO:	JWS14_04590(mshC)

RGO:	KYT97_04675(mshC)

ROZ:	CBI38_13440

RPSK:

JWS13_41840(mshC)

RGOR:

NMQ04_10235(mshC)

RANT:

RHODO2019_07815(mshC)

RHOP:

D8W71_16950(mshC)

REQ:	REQ_22990(mshC)

WHR:	OG579_02335(mshC)

GBR:

Gbro_2442

GPO:	GPOL_c23790(mshC)

GOR:

KTR9_2351

GOQ:	ACH46_10420

GTA:	BCM27_12530

GOC:	CXX93_12580(mshC)

GIT:	C6V83_11530(mshC)

GRU:	GCWB2_13135(mshC)

GOM:	D7316_00983(mshC)

GAV:	C5O27_14950(mshC)

GOD:	GKZ92_11900(mshC)

GAM:	GII34_11890(mshC)

GPD:	GII33_11175(mshC)

GJI:	H1R19_11725(mshC)

GOI:	LK459_21580(mshC)

GHN:	MVF96_11940(mshC)

GAMI:

IHQ52_15635(mshC)

GMG:	NWF22_22250(mshC)

GSI:	P5P27_09355(mshC)

SPIN:

KV203_09790(mshC)

TPR:

Tpau_2140

TSM:	ASU32_11680

TPUL:

TPB0596_24440(mshC)

TSD:	MTP03_24260(mshC)

SRT:

Srot_1222

DTM:	BJL86_1644

DIT:	C3V38_13760(mshC)

DIZ:	CT688_07945(mshC)

DPC:	A6048_08200

DLU:	A6035_07790

DKN:	NHB83_07500(mshC)

CBQ:	AL705_00170

TOY:	FO059_08695(mshC)

SCO:	SCO1663(cysS)

SALB:

XNR_5164

SMA:	SAVERM_6647(mshC)

SGR:	SGR_5840(cysS2)

SGB:

WQO_06085

SFI:

SFUL_1182

SANL:

KZO11_05740(mshC)

SGRF:

SGFS_090900

SCYE:

R2B67_30355(mshC)

SCB:	SCAB_73151(cysS2)

SSX:	SACTE_1098

SVL:	Strvi_6678

SHY:

SHJG_3103

SHO:	SHJGH_2867

SRC:	M271_38390

SMAL:

SMALA_1465

SSOI:

I1A49_10880

SFA:

Sfla_5170

SBH:	SBI_08453(cysS2)

SVE:

SVEN_1258

SDV:	BN159_6915(mshC)

SALS:

SLNWT_6236

STRP:

F750_1464(mshC)

SCI:	B446_08590

SLV:	SLIV_29430(mshC)

SGU:	SGLAU_06905(mshC)

SVT:	SVTN_07760

STRE:

GZL_06994

SCW:	TU94_06995

SLD:

T261_6564

SLC:	SL103_11145

SXI:	SXIM_04930

STRM:

M444_08655

STRC:

AA958_04125

SAMB:

SAM23877_1720(mshC)

SPRI:

SPRI_5889

SCZ:	ABE83_29740

SCX:	AS200_35140

SRW:	TUE45_02094(mshC_2)

STRF:

ASR50_07950

SLE:	sle_54540(sle_54540)

SRN:	A4G23_00897(mshC)

SPAV:

Spa2297_06465

STRT:

A8713_05930

SCLF:

BB341_23530

SGS:	AVL59_34935

STSI:

A4E84_08295

SKY:	D0C37_27810(mshC)

SALW:

CP975_06560

SGM:	GCM10017557_66480(mshC)

SCAD:

DN051_30320

SPHW:

NFX46_28840(mshC)

SLS:	SLINC_1891

SNR:	SNOUR_32045(mshC)

SALU:

DC74_2143

SALL:

SAZ_11290

SPLU:

LK06_005125

STRD:

NI25_30875

SNW:	BBN63_28220

SAUO:

BV401_11200

SSIA:

A7J05_28715

SVU:	B1H20_05270

SPUN:

BFF78_33200

SGV:	B1H19_10275

SLAU:

SLA_1199

SALF:

SMD44_01725

SALJ:

SMD11_5351

SLX:	SLAV_29190(mshC)

STRO:

STRMOE7_08985

SFK:

KY5_1496c

SNZ:	DC008_06370

SGE:	DWG14_06800(mshC_1)

SRJ:

SRO_5805

SLK:	SLUN_07790(mshC)

SDX:	C4B68_32345

SGD:	ELQ87_31345

SQZ:	FQU76_05085

SCYA:

EJ357_09370

SAST:

CD934_27385

SNQ:	CP978_07900

STIR:

DDW44_02350

SKA:	CP970_35210

SGZ:	C0216_21720

SVN:	CP980_26435

SNK:	CP967_28085

SHAW:

CEB94_08720(mshC)

SRK:	FGW37_06820

SFIC:

EIZ62_26930

SGAL:

CP966_06810(mshC)

SSPO:

DDQ41_01815

SVR:	CP971_26425

SPAD:

DVK44_04260

SFY:	GFH48_32030(mshC)

SAQU:

EJC51_11115

SGF:	HEP81_06112(mshC)

SCAV:

CVT27_04915

SSEO:

D0Z67_05250

SBY:	H7H31_04970

SRIM:

CP984_33090

SSUB:

CP968_26280

SPHV:

F9278_08030

SCIN:

CP977_07285

SFUG:

CNQ36_07005(mshC)

SPAC:

B1H29_29250

STSU:

B7R87_05085

SCHA:

CP983_34670

SGX:	H4W23_08220

SCOE:

CP976_09405(mshC)

SBAT:

G4Z16_28645

SSPB:

CP982_09730(mshC)

SNF:	JYK04_02319(mshC_1)

SPLA:

CP981_08100(mshC)

SBRO:

GQF42_11000

STUI:

GCM10017668_12400(mshC)

SATA:

C5746_08855

SCHF:

IPT68_07390

SHUN:

DWB77_06080(mshC_1)

SHAR:

HUT13_03200

SCYG:

S1361_09170(mshC)

SFEU:

IM697_14230

SLIA:

HA039_27795

SDW:	K7C20_07200(mshC)

SVD:	CP969_09300(mshC)

SCAL:

I6J39_05440

SAUH:

SU9_006135(mshC)

SFB:	CP974_04675

SMOB:

J7W19_06070(mshC)

SPRA:

CP972_07430(mshC)

SPEU:

CGZ69_06820

SROI:

IAG44_32110(mshC)

SXN:	IAG42_28700

SGJ:	IAG43_05670

SHK:	J2N69_06505(mshC)

SANU:

K7396_28615(mshC)

STRY:

EQG64_04920

SDD:	D9753_28545(mshC)

SACT:

DMT42_07355

SMAO:

CAG99_24840

SGOB:

test1122_01400(mshC)

SINE:

KI385_10485(mshC)

SLF:	JEQ17_37020(mshC)

SDEC:

L3078_09530(mshC)

SAOV:

G3H79_30340(mshC)

SDUR:

M4V62_33540(mshC)

SYAN:

NRK68_07395(mshC)

SRUG:

F0345_04400(mshC)

SAKB:

K1J60_35960(mshC)

STAA:

LDH80_31190(mshC)

SNIG:

HEK616_18850(mshC)

SVIO:

HWN34_26085(mshC)

SCAE:

IHE65_36050(mshC)

STUB:

MMF93_05930(mshC)

SSPN:

LXH13_08330(mshC)

SCIR:

STRCI_001739(mshC)

SXT:	KPP03845_101895(mshC)

SLON:

LGI35_11610(mshC)

SDRZ:

NEH16_25750(mshC)

STUD:

STRTU_005756(mshC)

STEE:

F3L20_09530(mshC)

SENG:

OJ254_20075(mshC)

SGK:	PET44_07245(mshC)

SANT:

QR300_33990(mshC)

SYUN:

MOV08_32080(mshC)

SARG:

HKX69_27870

SJN:	RI060_34135(mshC)

SCYN:

N8I84_09105(mshC)

SOV:	QZH56_28950(mshC)

SFIY:

F0344_30150

SHAU:

K9S39_34845(mshC)

SKG:	KJK29_31010(mshC)

SCOA:

QU709_35180(mshC)

SLAI:

P8A22_31525(mshC)

SROC:

RGF97_27735(mshC)

SFP:	QUY26_32390(mshC)

SCHG:

NRO40_05075(mshC)

STTN:

BSL84_07125

SINN:

ABB07_30335

STRZ:

OYE22_04775(mshC)

SCT:	SCAT_5134(cysS)

SCY:	SCATT_51280

KSK:	KSE_66390(mshC)

KAB:	B7C62_04985

KAU:	B6264_20980

KIT:	CFP65_1340

KIS:	HUT16_31320

STRI:

C7M71_027680

YIA:	LO772_27540(mshC)

STRH:

GXP74_15245

ABRY:

NYE86_11345(mshC)

AGLA:

OIE69_31135(mshC)

LXL:	KDY119_01677(mshC)

PSIC:

J4E96_07480

CMI:	CMM_1694(cysS2)

CMH:	VO01_07700

CMS:	CMS1669(cysS)

CMC:	CMN_01673(mshC)

CCAP:

AES38_08185

CZH:	H9X71_08505

CPHA:

FGI33_04680

CCAF:

FGD68_10895(mshC)

MCHN:

HCR76_07815

RTX:	TI83_06470

RTC:	APU90_10620

RTN:	A6122_1297

RRY:	C1O28_05855

RIA:	C7V51_04380

RFS:	C1I64_07810

RTE:	GSU10_07440

CUM:	NI26_08265

CUB:	BJK06_17815

CUG:	C1N91_08400

CCIT:

QPK07_08585(mshC)

FRP:	AX769_12405

FSB:	GCM10025867_27910(mshC)

CART:

PA27867_1750

CRY:	B7495_08560

CPHY:

B5808_08865

MYL:	C3E77_06795

MSPO:

KXZ72_03020(mshC)

SALC:

C2138_06035

SALD:

FVA74_05905

HUM:	DVJ78_12010

HUW:	FPZ11_01960

GRY:	D7I44_15720

PLAP:

EAO79_14315

LEU:	Leucomu_08505

LTR:	EVS81_01220

LEB:	G7066_06645

LDN:	H9L06_02590

LVI:	G7068_00385

LINS:

G7067_08535

LARI:

KI794_07435(mshC)

LALL:

MUN78_06300(mshC)

LRP:	MUN76_03305(mshC)

LTN:	KVY00_00390(mshC)

AGG:	C1N71_07065

AGRO:

JSQ78_02700

MANT:

BHD05_11320

HEA:	HL652_07655

FRN:	F1C15_02210

GLN:	F1C58_07115

AGX:	AGREI_1489(mshC)

SUBT:

KPL76_10935(mshC)

SUBA:

LQ955_06430(mshC)

PSEV:

USB125703_01901(mshC)

GSD:	M3M28_06325(mshC)

GMN:	GMOLON4_830(mshC)

LYH:	FrondiHNR_06890(mshC)

ART:

Arth_2168

ARR:	ARUE_c23240(cysS2)

ARM:

ART_0678

ARL:	AFL94_08190

ARE:	AL755_11770

AAQ:	AOC05_06935

ARW:	MB46_05370

ARH:	AHiyo8_63840

ARY:	ATC04_09710

ARZ:	AUT26_10710

ARU:	ASPU41_15915

ARQ:	BWQ92_21085

ARN:	CGK93_12595(mshC)

ARX:	ARZXY2_1811(mshC)

ACRY:

AC20117_18655

ARTH:

C3B78_10195(mshC)

ARTP:

E5206_12630(mshC)

AAGI:

NCTC2676_1_01361(mshC)

AJG:	KKR91_09040(mshC)

ASUN:

KG104_09475(mshC)

ASUF:

MNQ99_10580(mshC)

ARTY:

AHiyo4_24080

APOA:

J0916_06655

AWW:	G8758_05305(mshC)

ACAO:

NF551_08605(mshC)

AZH:	MUK71_08800(mshC)

AGEG:

MUG94_08765(mshC)

AOD:	Q8Z05_18910(mshC)

AKO:	N9A08_08310(mshC)

AZX:	N2K95_08635(mshC)

AJR:	N2K98_09240(mshC)

ARI:	UM93_04300

AAU:

AAur_2169

PUE:	FV140_10970(mshC)

PNV:	JMY29_10290(mshC)

ACH:

Achl_1911

APN:	Asphe3_18930

PSUL:

AU252_21645

PSNI:

NIBR502771_14905(mshC)

PSEY:

GU243_04615(mshC)

PDEL:

JCQ34_09565(mshC)

POK:	SMD14_10160(mshC)

AAI:	AARI_16310(cysS)

GAR:	AOZ07_08390

GCR:	GcLGCM259_1403(mshC)

GLU:	F0M17_08205(mshC)

GPR:	JQN66_08360

GMI:	NMP99_08425(mshC)

GMY:	XH9_00485(mshC)

GNC:	QQS42_08215(mshC)

RSA:	RSal33209_2044

KRH:	KRH_13920(mshC)

KPL:	KPaMU14_06485

KFV:	AS188_10740

KII:	KocCE7_07135

KRS:	EQG70_09815

KOD:	HBK84_07300

KVR:	CIB50_0001736(mshC)

MLU:	Mlut_11810

MICK:

B1A86_00005835

MYU:	M8233_05595(mshC)

MLY:	CJ228_003675(mshC)

MPOR:

KW076_08130(mshC)

MTEU:

R3I42_03950(mshC)

RMU:	RMDY18_10290

RDN:	HMPREF0733_11956(mshC)

RAJ:	RA11412_1933

RTER:

IDM49_06450

RAMA:

IDM48_06295

RKR:	I6G21_09310

SATK:

SA2016_2029

NAE:	BHE16_02885

AUL:	DCC27_003615

AIG:	QDX25_02890(mshC)

CMUA:

P8192_07860(mshC)

PAEY:

KUF55_09545(mshC)

PALU:

CJ193_001115(mshC)

BCV:

Bcav_2247

BFA:	Bfae_16240

BRX:	BH708_03560

BGG:	CFK41_08625

BRZ:	CFK38_10830

BSAU:

DWV08_05610

BRR:	C1N80_10835

BKI:	M4486_05405(mshC)

BRHA:

NLU66_11780(mshC)

BHH:	Bra3105_02190(mshC)

BHQ:	BRM3_11865(mshC)

HMM:	R3I40_05105(mshC)

JDE:

Jden_1195

KSE:	Ksed_13890

DNI:	HX89_07480

DAY:	FV141_06700

DERM:

H7F30_10770

LMOI:

VV02_16035

YIM:	J5M86_07305

ALX:	LVQ62_12370(mshC)

BEI:	GCM100_15530(mshC)

XCE:

Xcel_1479

XYL:	ET495_03570

XYA:	ET471_13165

IVA:	Isova_1436

IDO:	I598_0779(mshC)

CET:	B8281_12280

CCEU:

CBR64_19435

CPRT:

FIC82_011050

SKE:	Sked_20970

SANW:

G7063_07140

CFL:

Cfla_2010

CFI:

Celf_1780

CGA:	Celgi_1846

CELZ:

E5225_07645

CEJ:	GC089_07950

CELH:

GXP71_05770

CIRA:

LFM56_07970(mshC)

CPAL:

F1D97_10965

CELC:

K5O09_09030(mshC)

CDON:

KKR89_10170(mshC)

CWAN:

KG103_10260(mshC)

CFEN:

KG102_07790(mshC)

CWN:	NP075_10525(mshC)

CCHE:

NP064_06635(mshC)

CXIE:

NP048_10210(mshC)

OEK:	FFI11_012645

PSEI:

GCE65_06280

PSEK:

GCM125_29900(mshC)

ICA:	Intca_1753

ARS:	ADJ73_12730

JTE:	ASJ30_07950

JLI:	EXU32_07305

JME:	EEW87_007310

JAY:	H7A72_08100

JCR:	O9K63_04860(mshC)

TEH:	GKE56_04590

PHW:	G7075_06625

PEI:	H9L10_05830

TED:	U5C87_14480(mshC)

SERJ:

SGUI_0324

SERW:

FY030_08030

ORN:	DV701_02720

ORZ:	FNH13_10975

ORD:	L0A91_04025(mshC)

OCP:	NF557_09130(mshC)

BLY:	A2T55_09360

BLIN:

BLSMQ_2091

BRI:	FDF13_11170

BLUT:

EW640_03405

BCAU:

I6G59_12860

BSPO:

L1F31_09585(mshC)

DCO:	SAMEA4475696_1724(mshC)

GEZ:	FE251_06850

GYU:	FE374_07380

HALT:

IM660_08795

RHAL:

LQF10_08255(mshC)

RSUA:

LQF12_07355(mshC)

PFR:	PFREUD_14130(cysS1)

PFRE:

RM25_1334

PACD:

EGX94_02085

PAUS:

NCTC13651_02249(mshC)

PPC:	HMPREF9154_2051(mshC)

ARUB:

J5A65_05585

MPH:	MLP_17040(mshC)

MIK:	FOE78_16675

MICG:

GJV80_10680

TFL:	RPIT_08310

TFA:	BW733_01015 BW733_14760

TES:	BW730_02915

TEZ:	BKM78_02620

TDF:	H9L22_07335

TLA:	TLA_TLA_00532(mshC)

TEJ:	KDB89_05215(mshC)

RAIN:

Rai3103_16040

PRV:	G7070_14165

BRT:	J4N02_09830

PROP:

QQ658_08170(mshC)

BROO:

brsh051_03350(mshC)

NCA:

Noca_2628

NDK:	I601_3334(mshC_1) I601_4058(mshC_2)

NOY:	EXE57_01800

NOI:	FCL41_08395

NOO:	FE634_10325

NDP:	E2C04_08995

NSN:	EXE58_17755

NBE:	Back2_02160(cysS_1)

NANO:

G5V58_10885

NMES:

H9L09_20405

NARO:

CFH99_12690

NPI:	G7071_10980 G7071_14965

NAQU:

ENKNEFLB_02420(mshC_1) ENKNEFLB_03748(mshC_2)

NRO:	K8W59_08845(mshC) K8W59_12315

NOQ:	LN652_01860(mshC)

NCQ:	K6T13_04750 K6T13_08975(mshC)

NPC:	KUV85_04960(mshC) KUV85_06590

NPS:	KRR39_08465(mshC)

NMAR:

HPC71_10480

NFC:	KG111_09170(mshC)

PSIM:

KR76_13260

AER:	AERYTH_09025

AEZ:	C3E78_09230

AEB:	C6I20_06855

AEF:	GEV26_08635

ASEZ:

H9L21_08240

ADB:	NP095_08450(mshC)

AWN:	NQV15_09390(mshC)

MGG:	MPLG2_1802(mshC)

MUZ:	H4N58_10420

SGRG:

L0C25_21280(mshC)

NBT:	KLP28_16090(mshC)

KFL:

Kfla_3804

KQI:	F1D05_02930

KSL:	OG809_33465(mshC)

TFU:

Tfu_1832

THAO:

NI17_011180(mshC)

TALX:

FOF52_08720(mshC)

NDA:

Ndas_1544

NAL:	B005_4133(mshC)

NGV:	CDO52_17390

NEX:	NE857_14165(mshC)

NAKE:

KGD83_18125(mshC)

NCG:	KGD84_20455(mshC)

STRR:

EKD16_14115(mshC)

SNAH:

OUQ99_19695(mshC)

SAIU:

J4H86_01205

TCU:	Tcur_0549 Tcur_2287

ACTW:

F7P10_00750

AGRA:

AGRA3207_000515

AMAZ:

LUW76_03760(mshC) LUW76_33975

ACIR:

OG979_02255 OG979_37655(mshC)

SPIQ:

OHA34_02365(mshC)

ACTQ:

OG417_28215(mshC)

SRO:

Sros_5930

NOA:	BKM31_06205

NOW:	GBF35_32330

NCX:	Nocox_26285(mshC)

NGN:	LCN96_35405(mshC)

NFS:	OIE67_13085(mshC)

TBI:

Tbis_1932

MHAI:

OHB01_28485 OHB01_34120(mshC)

FRA:	Francci3_2642

FRE:	Franean1_4892

FAL:	FRAAL2858(cysS2)

FSY:	FsymDg_2272

FRI:	FraEuI1c_3764

ACE:

Acel_1172

NML:	Namu_2573 Namu_2780

NAK:	EH165_07205

NAKA:

H7F38_16865

GOB:	Gobs_2219 Gobs_2668

BSD:	BLASA_2691(cysS2)

MMAR:

MODMU_2589(cysS)

KRA:

Krad_1836

SEN:	SACE_2226(cysS2)

SACG:

FDZ84_02050

SVI:	Svir_22240 Svir_22970

SACC:

EYD13_12375(mshC1) EYD13_12820(mshC2)

AMD:	AMED_4930(cysS)

AMN:

RAM_25095

AMM:	AMES_4871(cysS)

AMZ:	B737_4871(cysS)

AOI:	AORI_3794(cysS)

AJA:	AJAP_20185(mshC)

AMQ:	AMETH_3568(cysS)

AMYC:

CU254_18120(mshC)

AMYB:

BKN51_11450

AAB:	A4R43_10960

AMYY:

YIM_27465(mshC)

AORI:

SD37_19470

AACD:

LWP59_15130(mshC)

APRT:

MUY14_12985(mshC)

AROO:

NQK81_03620(mshC)

ATHM:

L1857_20645(mshC)

ARHD:

VSH64_13385(mshC)

PDX:

Psed_3330

PSEA:

WY02_02660 WY02_17645

PSEE:

FRP1_12340

PSEH:

XF36_10280

PSEQ:

AD006_18630

PECQ:

AD017_26470

PHH:	AFB00_26255

PAUT:

Pdca_33300(cysS_1)

PBRO:

HOP40_01085 HOP40_24850

PPEL:

H6H00_01660 H6H00_23545

APRE:

CNX65_11215

AMI:

Amir_2201

SESP:

BN6_31410(mshC)

SSYI:

EKG83_34340

KAL:

KALB_3015

KUT:	JJ691_05060(cysS_1)

KBU:	Q4V64_10190(mshC)

KPHY:

AOZ06_20310

LED:	BBK82_45445

AHM:	TL08_13190

ACTI:

UA75_14180

ACAD:

UA74_14095

AHG:	AHOG_13260(mshC)

ACTA:

C1701_22465

ALO:

CRK57420

PMAD:

BAY61_17045 BAY61_17650

SACE:

GIY23_09785

ACTU:

Actkin_02750(mshC)

CROS:

N8J89_22050(mshC)

ACTY:

OG774_05090(mshC) OG774_10410 OG774_29155

UME:	RM788_10475(mshC)

STP:	Strop_2168

SAQ:

Sare_2322

MAU:	Micau_2918

MIL:

ML5_5478

MICB:

MicB006_6032

MTUA:

CSH63_10490

MICH:

FJK98_19510

MTEM:

GCE86_06865

MCAB:

HXZ27_17670

MSAG:

GCM10017556_49050(cysS)

VMA:	VAB18032_22945

MCRA:

ID554_00870

VER:	HUT12_19775

MENO:

Jiend_63480(cysS)

MCHL:

PVK74_04850(mshC) PVK74_30080

MFEU:

H1D33_19655(mshC)

MPRN:

Q3V37_21120(mshC)

MHAW:

RMN56_31485(mshC)

ASE:	ACPL_5266(mshC)

AMS:	AMIS_53910(mshC)

ACTN:

L083_5113(cysS)

AFS:

AFR_26630

ACTS:

ACWT_5136

AIH:	Aiant_10450(cysS_1)

ACTR:

Asp14428_13620(mshC_1) Asp14428_78950(mshC_2)

ACTL:

L3i22_075690(cysS)

ASIC:

Q0Z83_005320(mshC)

AOU:	ACTOB_005812(mshC)

PLK:	CIK06_13890

PLAB:

C6361_03060

PLAT:

C6W10_18570

PFLA:

Pflav_019260

PSUU:

Psuf_077890(cysS)

ATL:	Athai_13820(cysS_2) Athai_31580(mshC)

ASER:

Asera_43170(mshC)

PRY:	Prubr_68480(cysS_2)

CATI:

CS0771_69090(cysS)

DVC:	Dvina_37155

DFU:	Dfulv_17585(mshC)

DROS:

Drose_17230(mshC)

DAUR:

Daura_37135(mshC)

DMAT:

Dmats_31885(mshC)

NHY:	JQS43_13525

CCAZ:

COUCH_16815 COUCH_18220(mshC)

CAI:

Caci_5399

SNA:

Snas_4502

NAV:	JQS30_07435

GLY:	K3N28_19355(mshC)

TBW:	NCTC13354_00048(mshC)

ASG:	FB03_01895

AUU:	CJ184_007265

ATIM:

CYJ17_0007130

ACTT:

DDD63_05780

FSL:	EJO69_01710

FLH:	EJ997_02785

AEY:	CDG81_14280

PLAN:

A1s21148_03600

PLAK:

A1s21155_03415

PLIM:

PHILAsVB114_03465

PSUF:

A1sIA56_03320

PVS:	A1sIA79_03235

PVN:	A7sIIA15_03620

ABAM:

B1s21122_03285

NHI:	B1s21160_03160

NAB:	B1sIIB91_02980

EKE:	EK0264_18505

JTL:	M6D93_09965(mshC)

ABAI:

IMCC26256_11974

AFO:

Afer_1230

AYM:	YM304_19700(mshC)

ATQ:	GH723_08475

AQZ:	KSP35_02995

IAM:	HC251_13965

IMA:	PO878_09360(cysS) PO878_17665

DHI:	LH044_01055(cysS)

RRS:	RoseRS_3331

RCA:

Rcas_4315

CAU:

Caur_0747

CHL:	Chy400_0810

CAG:

Cagg_2860

HAU:

Haur_4263

KBS:	EPA93_27875

KBB:	ccbrp13_43660(cysS_2)

TFLA:

O0235_14015(cysS)

TTR:

Tter_1206

TRO:	trd_1909(cysS)

STI:

Sthe_3518

» show all

Reference

1 [PMID:17848100]

Authors

Fan F, Luxenburger A, Painter GF, Blanchard JS

Title

Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC).

Journal

Biochemistry 46:11421-9 (2007)
DOI:10.1021/bi7011492

Sequence

[msm:MSMEG_4189]

Reference

2 [PMID:16908186]

Authors

Gutierrez-Lugo MT, Newton GL, Fahey RC, Bewley CA

Title

Cloning, expression and rapid purification of active recombinant mycothiol ligase as B1 immunoglobulin binding domain of streptococcal protein G, glutathione-S-transferase and maltose binding protein fusion proteins in Mycobacterium smegmatis.

Journal

Protein Expr Purif 50:128-36 (2006)
DOI:10.1016/j.pep.2006.07.005

Reference

3 [PMID:19053270]

Authors

Tremblay LW, Fan F, Vetting MW, Blanchard JS

Title

The 1.6 A crystal structure of Mycobacterium smegmatis MshC: the penultimate enzyme in the mycothiol biosynthetic pathway.

Journal

Biochemistry 47:13326-35 (2008)
DOI:10.1021/bi801708f

Sequence

[msm:MSMEG_4189]