Entry
Name
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase;
L-delta-(alpha-aminoadipoyl)-L-cysteinyl-D-valine synthetase;
ACV synthetase;
L-alpha-aminoadipyl-cysteinyl-valine synthetase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
L-2-aminohexanedioate:L-cysteine:L-valine ligase (AMP-forming, valine-inverting)
Reaction(IUBMB)
3 ATP + L-2-aminohexanedioate + L-cysteine + L-valine + H2O = 3 AMP + 3 diphosphate + N-[L-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine [RN:
R04870 ]
Reaction(KEGG)
Substrate
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
N-[L-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine [CPD:
C05556 ]
Comment
Requires Mg2+. The enzyme contains 4'-phosphopantetheine, which may be involved in the mechanism of the reaction. Forms part of the penicillin biosynthesis pathway (for pathway, click here).
History
EC 6.3.2.26 created 2002
Pathway
ec00311 Penicillin and cephalosporin biosynthesis
ec01110 Biosynthesis of secondary metabolites
Orthology
K12743 N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase
Genes
PMEO : 129593796 129595266
» show all
Taxonomy
Reference
Authors
Byford MF, Baldwin JE, Shiau CY, Schofield CJ.
Title
The Mechanism of ACV Synthetase.
Journal
Reference
Authors
Theilgaard HB, Kristiansen KN, Henriksen CM, Nielsen J.
Title
Purification and characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Penicillium chrysogenum.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.26
ExPASy - ENZYME nomenclature database: 6.3.2.26