Entry
Name
coenzyme F420-0:L-glutamate ligase;
CofE-AF;
MJ0768;
CofE
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
L-glutamate:coenzyme F420-0 ligase (GDP-forming)
Reaction(IUBMB)
GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1 [RN:
R09399 ]
Reaction(KEGG)
Substrate
Product
Comment
This protein catalyses the successive addition of two glutamate residues to factor F420 (coenzyme F420) by two distinct and independent reactions. In the reaction described here the enzyme attaches a glutamate via its alpha-amine group to F420-0. In the second reaction (EC
6.3.2.34 , coenzyme F420-1:gamma-L-glutamate ligase) it catalyses the addition of a second L-glutamate residue to the gamma-carboxyl of the first glutamate.
History
EC 6.3.2.31 created 2010
Pathway
ec01120 Microbial metabolism in diverse environments
Orthology
K12234 coenzyme F420-0:L-glutamate ligase / coenzyme F420-1:gamma-L-glutamate ligase
K24998 dehydro coenzyme F420 reductase / coenzyme F420-0:L-glutamate ligase / coenzyme F420-1:gamma-L-glutamate ligase
Genes
NRE : BES08_02705 BES08_26370
SCH : Sphch_2140 Sphch_2202
SPHJ : BSL82_04905 BSL82_08910
MMAE : MMARE11_12460(fbiB)
MBAI : MB901379_01171(fbiB)
MHEK : JMUB5695_03655(fbiB)
MKY : IWGMT90018_13200(fbiB)
MPHL : MPHLCCUG_01626(fbiB_3)
MSAL : DSM43276_03406(fbiB_2)
MTER : 4434518_02998(fbiB)
NWL : NWFMUON74_13470(fbiB)
NSPU : IFM12276_09360(fbiB)
RKO : JWS14_25820 JWS14_34320
TPUL : TPB0596_34400(fbiB)
SHO : SHJGH_4267 SHJGH_5926
SAMB : SAM23877_3079(fbiB)
SLE : sle_42440(sle_42440)
SGM : GCM10017557_50170(fbiB)
SDX : C4B68_06930 C4B68_24085
SPAD : DVK44_11200 DVK44_18510
SGF : HEP81_01492 HEP81_04779
STUI : GCM10017668_27350(fbiB)
SHUN : DWB77_04646(fbiB_2)
SXT : KPP03845_103148(fbiB_1)
KAB : B7C62_12015 B7C62_12850
AMAU : DSM26151_28160(fbiB_1) DSM26151_28190(fbiB_2)
MHUM : NNL39_01335(cofE) NNL39_01525(cofE)
PSEK : GCM125_00170 GCM125_32860
NAQU : ENKNEFLB_03315(fbiB)
NEX : NE857_28265 NE857_32350(cofE)
ACTI : UA75_23080 UA75_27205
ACAD : UA74_22560 UA74_26615
ACTU : Actkin_05520(fbiB_3)
STP : Strop_0943 Strop_1139 Strop_1147
MSAG : GCM10017556_35200(fbiB)
BALA : DSM104299_01948(fbiB)
SBAE : DSM104329_01861(fbiB_1)
MEHF : MmiHf6_12340(fbiB_1)
MEES : MmiEs2_14490(fbiB_2)
MPD : MCP_1297(cofE-1) MCP_2474(cofE-2)
HSU : HLASF_0415 HLASF_1128
HSF : HLASA_0414 HLASA_1117
HHI : HAH_2869(cofE) HAH_4273
HHN : HISP_14585 HISP_16585
HAB : SG26_00985 SG26_06725
HDS : HSR122_0386(cofE) HSR122_2881(cofE2)
HAE : halTADL_0320 halTADL_1345
HDF : AArcSl_2462(cofE) AArcSl_2524(cofE2)
NARA : QQ977_07085 QQ977_13015
LOKI : Lokiarch_23940(cofE_1) Lokiarch_46970(cofE_3)
» show all
Taxonomy
Reference
Authors
Li H, Graupner M, Xu H, White RH
Title
CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii.
Journal
Sequence
Reference
Authors
Nocek B, Evdokimova E, Proudfoot M, Kudritska M, Grochowski LL, White RH, Savchenko A, Yakunin AF, Edwards A, Joachimiak A
Title
Structure of an amide bond forming F(420):gamma-glutamyl ligase from Archaeoglobus fulgidus -- a member of a new family of non-ribosomal peptide synthases.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.31
ExPASy - ENZYME nomenclature database: 6.3.2.31