KEGG ENZYME: 6.3.2.33

ENZYME: 6.3.2.33

Entry

EC 6.3.2.33                 Enzyme

Name

tetrahydrosarcinapterin synthase;
H4MPT:alpha-L-glutamate ligase;
MJ0620;
MptN protein

Class

Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)

Sysname

tetrahydromethanopterin:alpha-L-glutamate ligase (ADP-forming)

Reaction(IUBMB)

ATP + tetrahydromethanopterin + L-glutamate = ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin [RN:R09594]

Reaction(KEGG)

R09594

Substrate

ATP [CPD:C00002];
tetrahydromethanopterin [CPD:C01217];
L-glutamate [CPD:C00025]

Product

ADP [CPD:C00008];
phosphate [CPD:C00009];
5,6,7,8-tetrahydrosarcinapterin [CPD:C18802]

Comment

This enzyme catalyses the biosynthesis of 5,6,7,8-tetrahydrosarcinapterin, a modified form of tetrahydromethanopterin found in the Methanosarcinales. It does not require K+, and does not discriminate between ATP and GTP [1].

History

EC 6.3.2.33 created 2010

Pathway

ec00790

Folate biosynthesis

Orthology

K15740

tetrahydromethanopterin:alpha-L-glutamate ligase

Genes

PROD:

PCO85_01115

PAQU:

DMB82_0020250

PIN:

Ping_2322

SPOI:

IMCC21906_01613

SNAN:

I6N98_15575

MCA:

MCA2864

METU:

GNH96_03525

MMEO:

OOT43_14010

MMT:	Metme_2266

MDN:	JT25_019340

MDH:	AYM39_08545

MKO:	MKLM6_1779

METL:

U737_14815

MPAD:

KEF85_11470

MELL:

IVG45_03935

MRP:	NM686_014185

MMOT:

QZJ86_13650

MAH:	MEALZ_2426

MBUR:

EQU24_09120

MPSY:

CEK71_02665

MMAI:

sS8_3393

MSZE:

MSZNOR_2916

MMOB:

F6R98_19015

MEIN:

methR_P2100

MOZ:	MoryE10_28860

MISZ:

MishRS11D_40140

MESL:

KKZ03_09090

MEIY:

MIN45_P1842

MCAU:

MIT9_P1442

TCX:

Tcr_1768

HTR:	EPV75_09480

TCY:	Thicy_0311

TAO:	THIAE_08710

THIO:

AYJ59_04455

TMH:	JX580_01585

MEJ:

Q7A_45

MEC:

Q7C_1773

MPIN:

LGT42_002850

MMAF:

GCM100_17920

MTHA:

VSX76_15980

TXA:	HQN79_09935

TSE:	THMIRHAS_05250

TZO:	THMIRHAT_19060

BLEP:

AL038_08770

NOC:

Noc_0259

NHL:

Nhal_3877

NWA:

Nwat_0360

NWR:	E3U44_04670

NTT:

TAO_1754

NTG:	NSCAC_0081(mptN)

SEDS:

AAY24_01655

HAM:

HALO3126

HAF:	C8233_03465

HPRO:

LMS44_07885

HHAO:

QWG60_15160

HVN:	EI420_12615

HOL:	HORIV_23050

HNP:	SR894_06700

HTT:	HZS52_20065

CAMH:

LCW13_07760

COBE:

CLAM6_14860

COBB:

H2O77_07785

MMW:	Mmwyl1_0157

MME:	Marme_1385

MPC:	Mar181_0123

MPRI:

MP3633_0137

MFOI:

JSY38_09950

MRHI:

KDW99_00720

MPON:

MACH16_01480

NIK:	F5I99_17995

OME:	OLMES_4802

EMP:	EZMO1_4142(rimK)

COX:	E0W60_31935

BUG:	BC1001_3949

BYI:	BYI23_B014450(rimK)

BUE:	BRPE67_BCDS01440(rimK)

BUQ:	AC233_22030

BPH:	Bphy_5945 Bphy_6485

BGE:	BC1002_3222

BPX:	BUPH_00533

BCAI:

K788_0001557

PSPW:

BJG93_19400

PHS:	C2L64_21810

PTER:

C2L65_26160 C2L65_34285

PSAA:

QEN71_34080

BUO:	BRPE64_BCDS01580(rimK)

VAP:	Vapar_3081

VPD:	VAPA_1c31450

MPT:

Mpe_A2610

METP:

C1M51_15090

RGE:	RGE_40260(rimK)

LCH:

Lcho_3149

METR:

BSY238_3291

MFA:

Mfla_1657

MMB:

Mmol_1343

MEH:

M301_1553

MEI:	Msip34_1501

MEP:	MPQ_1564(rimK)

MBAC:

BN1209_0748(rimK)

MPAU:

ZMTM_11850

SPLB:

SFPGR_25970

AZA:	AZKH_p0272

AZD:	CDA09_11320

THU:	AC731_004850

THAU:

C4PIVTH_3699

THK:	CCZ27_13245

DDZ:	DSYM_25700

MCI:	Mesci_4261

MCIC:

A4R28_05750

MESW:

A9K65_023625

MESM:

EJ066_29725

MOH:	IHQ72_13870

MMEI:

LRP31_14000

PHT:	BLM14_23795

RGA:	RGR602_PC01569

NPM:	QEO92_19225

XAU:

Xaut_1789

XDI:	EZH22_07505

STAR:

G3545_09785

LNE:	FZC33_09340

SNO:

Snov_0749

ANC:	GBB76_00745

APRA:

G3A50_16490

APOL:

K9D25_20195

MPO:

Mpop_1783

MRD:	Mrad2831_0531

MET:

M446_5781

MNO:

Mnod_6005

MOR:

MOC_0686

MAQU:

Maq22A_c16480(rimK)

MPHY:

MCBMB27_00108

MEE:	DA075_05735

METD:

C0214_10530

METX:

A3862_28295

METS:

DK389_20660

METI:

DK427_18450

MMES:

MMSR116_16695

MTEA:

DK419_10800

MIND:

mvi_45920

MOG:	MMB17_20020

MTAD:

M6G65_13660

MSL:

Msil_2388

MTUN:

MTUNDRAET4_2724

MLG:	CWB41_06050

MEDK:

QEV83_11765

HDN:

Hden_1476

HDT:	HYPDE_31263

HMC:	HYPMC_1626

HNI:	W911_13180

FIL:	BN1229_v1_0585

FIY:	BN1229_v1_0587

MCG:

GL4_1785

METG:

HT051_10185

MSC:

BN69_1354

MBRY:

B1812_01095

MROS:

EHO51_13060

MHEY:

H2LOC_003820

MPAR:

F7D14_05055

MIWA:

SS37A_00080

MTW:	CQW49_13490

MECQ:

MSC49_12080

PLEO:

OHA_1_01034(rimK)

HDI:	HDIA_2313(lysX)

LAP:	ACP90_05820

LABR:

CHH27_14855

LABT:

FIU93_11455(lysX)

LAGG:

B0E33_17375

YAN:	AYJ57_18930

PABY:

Ga0080574_TMP3824

MALU:

KU6B_45100

TGL:	HFZ77_12215

PALX:

GQA70_00045

PSEW:

JHW44_10545

RHC:

RGUI_1595

RMB:	K529_021915

LIT:	FPZ52_13040

PALW:

PSAL_037890(rimK)

GBE:	GbCGDNIH1_0772

GBH:	GbCGDNIH2_0772

GBC:	GbCGDNIH3_0772

GBS:	GbCGDNIH4_0772

ALI:	AZOLI_p10699 AZOLI_p10888

ABQ:	ABAZ39_25475

ABF:	AMK58_22325

ATI:	AL072_27015

AZT:	TSH58p_01210

AZM:	DM194_17145

AOZ:	HUE56_08915

AARE:

D3093_24955

AZS:	E6C72_18240 E6C72_22390

MAGQ:

MGMAQ_0159

HCJ:

HCR_14150

DMA:

DMR_18070

DCB:	C3Y92_08250

DGG:

DGI_0284

DMS:	E8L03_06215

DSA:	Desal_3089

DHY:	DESAM_21527

DAF:	Desaf_1060

DAS:

Daes_1177

DPI:

BN4_12184

DEJ:	AWY79_17295

PSEL:

GM415_00290

DDN:	DND132_2927

DSX:	GD604_07115

DSD:	GD606_05870

DBA:

Dbac_2700

DESU:

NLA06_13430

DLI:

dnl_59780

DMM:	dnm_097390

DBR:

Deba_0382

ADE:

Adeh_1691

ACP:	A2cp1_2254

AFW:	Anae109_2113

ANK:	AnaeK_2165

BACW:

QR42_02385

ANX:	ACH33_11280

ATHE:

K3F53_07725

HAS:	Halsa_0948

SFB:	CP974_23765

ATL:	Athai_62040

PSL:

Psta_3185

PIR:	VN12_14460(lysX)

MFF:	MFFC18_17590(rimK_2)

RUV:	EC9_35470(lysX_1)

RCF:	Poly24_34890(rimK_2)

AHEL:

Q31a_62580(lysX_2)

LCRE:

Pla8534_62270(rimK_3)

AAGG:

ETAA8_05130(lysX)

BVO:	Pan97_33080(rimK_2)

BREM:

PSR63_21935

RLC:	K227x_27850(rimK_2)

SMAM:

Mal15_33990(rimK_2)

LPAV:

PLANPX_1004

LLH:	I41_11720(lysX)

AMUC:

Pan181_44390(lysX)

PND:	Pla175_40190(lysX)

PEH:	Spb1_33560(lysX)

PBS:	Plabr_4808

PLH:	VT85_05470(lysX)

FMR:	Fuma_00365(lysX_1)

GPN:	Pan110_11870(rimK_1)

GFM:	Enr17x_12900(rimK_1)

MRI:	Mal4_29790(rimK_1)

SDYN:

Mal52_18790(lysX)

PLON:

Pla110_18950(rimK_2)

TPOL:

Mal48_26090(rimK_1)

PVAR:

SH412_003488

CCOP:

Mal65_23790(lysX)

TIM:	GMBLW1_05850

LRS:	PX52LOC_02774

FTJ:

FTUN_8523

ULI:	ETAA1_18390(lysX)

PBOR:

BSF38_00162(lysX)

AGV:	OJF2_59290(lysX)

ARAM:

KAR29_10125

NLI:	G3M70_11550

MOX:

DAMO_0462

MJA:

MJ_0620

MFE:	Mefer_0208

MVU:	Metvu_0791

MFS:	MFS40622_1033

MIF:	Metin_0376

MJH:	JH146_0844

MESG:

MLAUSG7_0282(mptN)

MESA:

MLASG1_1392(mptN)

MIG:	Metig_0603

MMP:

MMP0702

MMQ:	MmarC5_0874

MMX:	MmarC6_0182

MMZ:	MmarC7_1729

MMD:

GYY_02745

MMAK:

MMKA1_07550(mptN)

MMAO:

MMOS7_07480(mptN)

MMAD:

MMJJ_02750(lysX_1)

MAE:

Maeo_0564

MVN:	Mevan_1580

MVO:

Mvol_0278

MOK:	Metok_1010

METF:

CFE53_02140

MBA:	Mbar_A3203

MBY:	MSBRM_1601

MBW:	MSBRW_1532

MBAR:

MSBR2_0806

MBAK:

MSBR3_0066

MAC:	MA_3268(rimK)

MMA:

MM_0111

MMAZ:

MmTuc01_0117

MMJ:	MSMAS_1220

MMAC:

MSMAC_2562

MVC:	MSVAZ_2319

MEK:	MSKOL_2330

MLS:	MSLAZ_1472

METM:

MSMTP_1203

MEF:	MSWH1_2073

MEQ:	MSWHS_3023

MSJ:	MSSAC_1749

MSZ:	MSSIH_2466

MSW:	MSSIT_2508

MTHR:

MSTHT_0138

MTHE:

MSTHC_0590

MHOR:

MSHOH_2484

MFZ:	AOB57_001770

MBU:	Mbur_1276(mptN)

MMET:

MCMEM_1723

MELO:

J7W08_07655

MMH:

Mmah_1581

MHAZ:

BHR79_05815

MPOT:

BKM01_01915

MEV:	Metev_0291

MZH:	Mzhil_1580

MPY:

Mpsy_2609

MZI:	HWN40_09855

MMAV:

RE476_00680(mptN)

MSEB:

RE474_10415(mptN)

MHZ:	Metho_1409

MTP:

Mthe_1118

MCJ:	MCON_0555(mptN)

MHI:

Mhar_2185

MHU:

Mhun_1105

MRTJ:

KHC33_08600

MLA:

Mlab_1175

MEM:	Memar_1015

MBG:	BN140_2303

MEMA:

MMAB1_3062

MCHK:

MchiMG62_11390

MSUM:

OH143_11800

MPI:

Mpet_1310

MFK:	E2N92_12970

MESB:

L1S32_02810

MPL:

Mpal_2364

PABI:

PABY_03160

PARE:

PYJP_15150

SSO:	SSO2346(rimK-3)

SOL:

Ssol_0158

SSOA:

SULA_0150

SSOL:

SULB_0151

SSOF:

SULC_0150

SSHI:

J5U23_01141

SACN:

SacN8_04820

SIS:	LS215_0174

SIA:	M1425_0143

SIM:	M1627_0143

SID:

M164_0162

SIY:	YG5714_0147

SIN:	YN1551_2746

SII:

LD85_0147

SIH:

SiH_0147

SIR:

SiRe_0143

SIC:

SiL_0134

SULA:

BFU36_10505

SULE:

GFS03_02265

SULO:

GFS33_08960

MHK:	DFR87_03205

MPRU:

DFR88_03120

MTEN:

GWK48_08920

SMET:

RQ359_001712

PIS:

Pisl_1747

VDI:

Vdis_2361

VMO:

VMUT_0713

BARC:

AOA65_2016(mptN)

LOKI:

Lokiarch_28770(mptN)

» show all

Reference

1 [PMID:12909715]

Authors

Li H, Xu H, Graham DE, White RH

Title

Glutathione synthetase homologs encode alpha-L-glutamate ligases for methanogenic coenzyme F420 and tetrahydrosarcinapterin biosyntheses.

Journal

Proc Natl Acad Sci U S A 100:9785-90 (2003)
DOI:10.1073/pnas.1733391100

Sequence

[mja:MJ_0620]

Other DBs

ExplorEnz - The Enzyme Database:

6.3.2.33

IUBMB Enzyme Nomenclature:

6.3.2.33

ExPASy - ENZYME nomenclature database:

6.3.2.33

BRENDA, the Enzyme Database:

6.3.2.33

DBGET integrated database retrieval system