Entry
Name
N2-citryl-N6-acetyl-N6-hydroxylysine synthase;
Nalpha-citryl-Nepsilon-acetyl-Nepsilon-hydroxylysine synthase;
iucA (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
citrate:N6-acetyl-N6-hydroxy-L-lysine ligase (AMP-forming)
Reaction(IUBMB)
2 ATP + citrate + N6-acetyl-N6-hydroxy-L-lysine + H2O = 2 ADP + 2 phosphate + N6-acetyl-N2-citryl-N6-hydroxy-L-lysine [RN:
R10090 ]
Reaction(KEGG)
Substrate
Product
ADP [CPD:
C00008 ];
phosphate [CPD:
C00009 ];
N6-acetyl-N2-citryl-N6-hydroxy-L-lysine
Comment
Requires Mg2+. The enzyme is involved in the biosynthesis of aerobactin, a dihydroxamate siderophore. It belongs to a class of siderophore synthases known as type A nonribosomal peptide synthase-independent synthases (NIS). Type A enzymes are responsible for the formation of amide or ester bonds between polyamines or amino alcohols and a prochiral carboxyl group of citrate. The enzyme is believed to form an adenylate intermediate prior to ligation.
History
EC 6.3.2.38 created 2012, modified 2019
Pathway
ec00997 Biosynthesis of various other secondary metabolites
ec01110 Biosynthesis of secondary metabolites
ec01120 Microbial metabolism in diverse environments
Orthology
K03894 N2-citryl-N6-acetyl-N6-hydroxylysine synthase
Genes
ECOO : ECRM13514_1290(iucA)
ECOH : ECRM13516_1246(iucA)
ECV : APECO1_O1CoBM172(iucA)
CLAP : NCTC11466_02781(iucA)
YPK : y3380(iucA1) y3381(iucA2)
YPP : YPDSF_0528 YPDSF_0529
YPZ : YPZ3_0748(iucA2) YPZ3_0749(iucA1)
YPT : A1122_00025 A1122_00030
YPD : YPD4_0703(iucA2) YPD4_0704(iucA1)
YPX : YPD8_0703(iucA2) YPD8_0704(iucA1)
YPI : YpsIP31758_0714(iucA)
SFJ : SAMEA4384070_0954(iucA)
SOF : NCTC11214_00578(iucA)
» show all
Taxonomy
Reference
Authors
Gibson F, Magrath DI.
Title
The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I.
Journal
Reference
2
Authors
Maurer, P.J. and Miller, M.
Title
Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N6-acetyl-N6-hydroxylysine.
Journal
J Am Chem Soc 104:3096-3101 (1982)
Reference
Authors
de Lorenzo V, Bindereif A, Paw BH, Neilands JB
Title
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.
Journal
Sequence
Reference
4
Authors
Appanna, D.L., Grundy, B.J., Szczepan, E.W. and Viswanatha, T.
Title
Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1.
Journal
Biochim Biophys Acta 801:437-443 (1984)
Reference
Authors
Challis GL.
Title
A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases.
Journal
Reference
Authors
Oves-Costales D, Kadi N, Challis GL
Title
The long-overlooked enzymology of a nonribosomal peptide synthetase-independent pathway for virulence-conferring siderophore biosynthesis.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.38
ExPASy - ENZYME nomenclature database: 6.3.2.38