KEGG ENZYME: 6.3.2.53

ENZYME: 6.3.2.53

Entry

EC 6.3.2.53                 Enzyme

Name

UDP-N-acetylmuramoyl-L-alanine---L-glutamate ligase;
murD2 (gene name);
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-glutamate synthetase;
UDP-MurNAc-L-Ala-L-Glu synthetase

Class

Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)

Sysname

UDP-N-acetylmuramoyl-L-alanine---L-glutamate ligase (ADP-forming)

Reaction(IUBMB)

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + L-glutamate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-glutamate [RN:R12272]

Reaction(KEGG)

R12272

Substrate

ATP [CPD:C00002];
UDP-N-acetyl-alpha-D-muramoyl-L-alanine [CPD:C01212];
L-glutamate [CPD:C00025]

Product

ADP [CPD:C00008];
phosphate [CPD:C00009];
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-glutamate [CPD:C22053]

Comment

The enzyme, characterized from the bacterium Xanthomonas oryzae, catalyses the ligation of a terminal L-glutamate to UDP-N-acetyl-alpha-D-muramoyl-L-alanine. The combined activity of this enzyme and EC 5.1.1.23, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-glutamate epimerase, provides an alternative route for incorporating D-glutamate into peptidoglycan, replacing the more common combination of EC 5.1.1.3, glutamate racemase, and EC 6.3.2.9, UDP-N-acetylmuramoyl-L-alanine---D-glutamate ligase.

History

EC 6.3.2.53 created 2018

Pathway

ec00550

Peptidoglycan biosynthesis

ec01100

Metabolic pathways

Orthology

K23169

UDP-N-acetylmuramoyl-L-alanine---L-glutamate ligase

Genes

XFA:

XF_1118

XFT:	PD_0410(murD)

XFM:	Xfasm12_0463

XFN:	XfasM23_0404

XFF:	XFLM_07445(murD)

XFL:	P303_01520(murD)

XFS:	D934_03165(murD)

XFH:	XFHB_09980(murD)

XCC:	XCC2738(murD)

XCB:

XC_1375

XCA:	xcc-b100_1423(murD)

XCP:	XCR_3103(murD)

XCV:	XCV3053(murD)

XAX:	XACM_2840(murD)

XAC:	XAC2908(murD)

XCI:	XCAW_03185(murD)

XCT:	J151_03093

XCJ:	J158_03072

XCU:	J159_03068

XCN:	J169_03091

XCW:	J162_03071

XCR:	J163_03068

XCM:	J164_03068

XCF:	J172_03084

XFU:	XFF4834R_chr17310(murD)

XAO:	XAC29_14785(murD)

XOM:	XOO1320(XOO1320)

XOO:	XOO1436(murD)

XOP:	PXO_02013(murD)

XOY:	AZ54_17285(murD)

XOR:	XOC_1508(murD)

XOZ:	BE73_15870(murD)

XAL:	XALC_1040(murD)

XSA:	SB85_19215(murD)

XTN:	FD63_12880(murD)

XFR:	BER92_13375

XVE:	BJD12_05415

XPE:	BJD13_02105

XHR:	XJ27_01680

XGA:	BI317_14830

XPH:	XppCFBP6546_21370(XppCFBP6546P_21370)

XVA:	C7V42_14840

XAN:	AC801_14450(murD)

XAR:	XB05_09285(murD)

XHY:	FZ025_02780

XCZ:	EBN15_12515

XTH:	G4Q83_06635

XEU:	XSP_002828

XHD:	LMG31886_14450(murD2)

XAG:	HEP73_01423

XDY:	NYR95_15630(murD)

XAS:	HEP74_01349

SML:	Smlt1170(murD)

SMT:

Smal_1013

BUJ:	BurJV3_1007

SMZ:	SMD_1091(murD)

SACZ:

AOT14_11460(murD)

STEK:

AXG53_12015(murD)

SRH:	BAY15_1165

SLM:	BIZ42_14380

STEN:

CCR98_05430

STEM:

CLM74_05420

STES:

MG068_05045

SINC:

DAIF1_10570(murD)

SPAQ:

STNY_R11340

STEQ:

ICJ04_04940

SGEN:

RKE57_05055(murD)

PSU:	Psesu_0813

PSUW:

WQ53_12435(murD)

PSD:	DSC_05875(murD)

PMEX:

H4W19_08625

PJP:	LAG73_03195(murD)

PDD:	MNQ95_10950(murD)

PWI:	MWN52_12500(murD)

LAB:	LA76x_1652(murD)

LAQ:	GLA29479_622(murD)

LCP:	LC55x_1440(murD)

LGU:	LG3211_3705(murD)

LEZ:	GLE_3730(murD)

LEM:	LEN_1404(murD)

LMB:	C9I47_0893

LYT:	DWG18_03455

LUE:	DCD74_07525

LYJ:	FKV23_04210

LSOL:

GOY17_14340

LSX:	H8B22_02545

LARE:

HIV01_000510

LHX:	LYSHEL_19050(murD)

LCAS:

LYSCAS_19050(murD)

LCIC:

INQ41_04415

LAVI:

INQ42_04185

LLZ:	LYB30171_00860(murD2)

LAUX:

LA521A_28280(murD)

LYA:	RDV84_14270(murD)

LUM:	CNR27_12535

LUS:	E5843_04845

LUG:	FPZ22_04125

THES:

FHQ07_12080

THEH:

G7079_04620

TCN:	H9L16_01115

TBV:	H9L17_12625

XBC:	ELE36_04540

FAU:	Fraau_2758

FSG:	LQ771_02330(murD)

FED:	LQ772_02830(murD)

RHD:	R2APBS1_3189

RGL:	CS053_04850

RTH:	LRK53_15935(murD)

DJI:	CH75_02000

DJA:	HY57_07400

DTX:	ATSB10_38180

DYE:	EO087_15600

DCS:	ISN74_03625

DTL:	H8F01_14060

DTA:	DYST_04094

DKO:

I596_815

LRZ:	BJI69_02825

LPY:	FIV34_16565

LANH:

KR767_11545(murD)

LFL:	IM816_03480

LAEG:

L2Y94_04620(murD)

LAEO:

L2Y97_17605(murD)

LAES:

L2Y96_17490(murD)

XBA:	C7S18_07690

RBD:

ALSL_0763

TAMN:

N4264_21450(murD)

WMA:	WM2015_2023

PHL:

KKY_2208

PEM:	OF122_05850(murD)

DEQ:	XM25_12460

DEI:	C4375_13235(murD)

DEA:	FPZ08_10315(murD)

DSAL:

K1X15_14525(murD)

DOY:	JI749_07465(murD)

DRH:	JI748_02225(murD)

MMYR:

MXMO3_01240

YTI:	FNA67_16185(murD)

MBRY:

B1812_02585

PBR:	PB2503_10389

ECOG:

FIV45_12580

PAQI:

KW060_11915(murD)

SUA:

Saut_0607

SPAL:

FM071_02350(murD)

FSA:	C5Q98_01705(murD)

ESR:

ES1_22010

ESU:

EUS_07940

BFI:

CIY_04060

RIX:

RO1_21100

RIM:

ROI_08450

LACY:

A4V08_15955

PXV:	FXF36_08210(murD)

CBOL:

CGC65_00545(murD)

LBW:	C3V36_02640

CBAR:

PATL70BA_0973(murD)

BULL:

JOS54_04620(murD)

MNE:	D174_13165

MYN:	MyAD_12925

MMAG:

MMAD_25840(murD)

MARZ:

MARA_13720(murD)

MAUB:

MAUB_03040(murD)

MPHU:

MPHO_09770(murD)

MMUC:

C1S78_013345(murD)

MGRO:

FZ046_18265

PSAI:

C3B54_111517

SALC:

C2138_03505

SALD:

FVA74_09830

LTR:	EVS81_15155(murD)

NOI:	FCL41_11765(murD)

NDP:	E2C04_12315(murD)

NMAR:

HPC71_14690(murD)

FSY:	FsymDg_3110

NAK:	EH165_15010(murD)

GOB:

Gobs_4629

STP:	Strop_3005

SAQ:

Sare_3229

MAU:	Micau_4222

MIL:

ML5_4080

MICB:

MicB006_3213

MTUA:

CSH63_03520

MICH:

FJK98_22630

MTEM:

GCE86_15450

MCAB:

HXZ27_21490

MSAG:

GCM10017556_04310(murD)

VMA:	VAB18032_25490(murD)

MCRA:

ID554_25220

VER:	HUT12_22125

MCHL:

PVK74_11170(murD)

MFEU:

H1D33_26675(murD)

MPRN:

Q3V37_17040(murD)

MHAW:

RMN56_22240(murD)

ASE:	ACPL_1938(murD)

AMS:	AMIS_16910

ACTN:

L083_2284(murD)

AFS:	AFR_11175(murD)

ACTS:

ACWT_1816

AIH:	Aiant_62820(murD)

ACTR:

Asp14428_22960

ACTL:

L3i22_019110(murD)

ASIC:

Q0Z83_070110(murD)

AOU:	ACTOB_001892(murD)

PLK:	CIK06_19240

PLAB:

C6361_35835

PLAT:

C6W10_06560

PFLA:

Pflav_048860(murD)

PSUU:

Psuf_006650(murD)

PRY:	Prubr_01400

DVC:	Dvina_14080

CCAZ:

COUCH_28460(murD)

SBAE:

DSM104329_01985(murD2)

AQZ:	KSP35_00795

MIN:	Minf_2246(murD)

MKC:

kam1_2041

MEAP:

MTHMO_2232(murD)

UAM:	UABAM_00997

PRF:	PeribacterA2_0609

MIB:	UY43_C0001G0610

» show all

Reference

1 [PMID:28294606]

Authors

Feng R, Satoh Y, Ogasawara Y, Yoshimura T, Dairi T

Title

A Glycopeptidyl-Glutamate Epimerase for Bacterial Peptidoglycan Biosynthesis.

Journal

J Am Chem Soc 139:4243-4245 (2017)
DOI:10.1021/jacs.7b01221

Sequence

[xom:XOO1320]

Other DBs

ExplorEnz - The Enzyme Database:

6.3.2.53

IUBMB Enzyme Nomenclature:

6.3.2.53

ExPASy - ENZYME nomenclature database:

6.3.2.53

BRENDA, the Enzyme Database:

6.3.2.53

DBGET integrated database retrieval system