KEGG ENZYME: 6.3.2.55

ENZYME: 6.3.2.55

Entry

EC 6.3.2.55                 Enzyme

Name

2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate synthase;
sbnF (gene name)

Class

Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)

Sysname

2-[(2-aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate:L-2,3-diaminopropanoate ligase {2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate-forming}

Reaction(IUBMB)

ATP + 2-[(2-aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate + L-2,3-diaminopropanoate = AMP + diphosphate + 2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate [RN:R12311]

Reaction(KEGG)

R12311

Substrate

ATP [CPD:C00002];
2-[(2-aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate [CPD:C22073];
L-2,3-diaminopropanoate [CPD:C03401]

Product

AMP [CPD:C00020];
diphosphate [CPD:C00013];
2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate [CPD:C22074]

Comment

Requires Mg2+. The enzyme, characterized from the bacterium Staphylococcus aureus, is involved in the biosynthesis of the siderophore staphyloferrin B. It belongs to a class of siderophore synthases known as type C nonribosomal peptide synthase-independent synthases (NIS). Type C NIS enzymes recognize esterified or amidated derivatives of carboxylic acids. The enzyme likely forms a 2-[(2-aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate adenylate intermediate prior to ligation.

History

EC 6.3.2.55 created 2019

Pathway

ec00997

Biosynthesis of various other secondary metabolites

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K23374

2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate synthase

Genes

DDD:	Dda3937_02851(acsC)

DZE:	Dd1591_2674

DDC:	Dd586_2581

DZC:	W909_07420

DSO:	A4U42_16815

CED:	LH89_04360

DFN:	CVE23_08420

DDQ:

DDI_1277

DAQ:	DAQ1742_01514(acsC)

DIC:	Dpoa569_002355

DLC:	O1Q98_19085

LBQ:	CKQ53_11530

LPOP:

I6N93_10210

SGL:

SGP1_0041

XDO:

XDD1_1966

VTA:

A3470

PFV:	Psefu_2383

PSB:

Psyr_2587

PSYR:

N018_14375

PSP:	PSPPH_2754

PSAV:

PSA3335_16160

PAMG:

BKM19_016035

PAVL:

BKM03_14810

PVD:	CFBP1590__2717

PCAB:

JGS08_13335

PCOF:

POR16_12185

PTRE:

I9H09_12725

PSYI:

MME58_13590

PCG:	AXG94_01630

PBC:	CD58_14505

PCH:	EY04_15705

PCZ:	PCL1606_28870

PCP:	JM49_14860

PCHP:

C4K32_3263

PSEM:

TO66_16020

PFK:	PFAS1_21425

PSIL:

PMA3_09135

PFIT:

KJY40_15385

PNB:	NK667_16650

PSKU:

KUIN1_25720

PALI:

A3K91_1226

PSYP:

E5677_07280

ABY:

ABAYE2005

ABC:	ACICU_01675

ABN:

AB57_1872

ABB:	ABBFA_01825(iucC_2)

ABX:

ABK1_2133

ABZ:	ABZJ_01836

ABR:	ABTJ_02032

ABD:	ABTW07_1891

ABH:

M3Q_2027

ABAD:

ABD1_16350

ABJ:	BJAB07104_02077

ABAB:

BJAB0715_01858

ABAJ:

BJAB0868_01797

ABAZ:

P795_9035

ABK:	LX00_08320

ABAU:

IX87_19070

ABAA:

IX88_10155

ABW:	BL01_08515

ABAL:

ABLAC_24810

ALC:	OTEC02_07345

ACI:

ACIAD2121

ACV:	AMD27_03895

ARJ:	DOM24_08495

ADS:	FPL17_05855

ATZ:	M5E07_06425

AVIV:

LF296_08360

ACIE:

KIP84_07255

CEK:	D0B88_14035

CEG:	D0C16_05365

MMT:	Metme_3393

CCAG:

SR908_12755

HEL:

HELO_3334

HHU:	AR456_14830

HHH:	CLM76_01230

HSV:	HNO53_08395

HBH:	E4T21_15090

HPIZ:

GYM47_13050

HAA:	A5892_12600

RSO:

RSp0422

RSE:	F504_3902(sbnF)

RPU:	CDC45_19740

RNC:	GO999_24080

RSL:	RPSI07_mp0383

RSN:	RSPO_m01406(rhbC)

RSM:	CMR15_mp10435

RSY:	RSUY_34900(iucC_2)

RSG:	JK151_14160

CNC:	CNE_2c14880(sbnA2)

CUH:	BJN34_08300

RME:

Rmet_1112

CUK:	KB879_12580

BPLA:

bpln_2g05060

MFLA:

GO485_03160

RUG:	QC826_07720

NET:

Neut_1745

NLC:	EBAPG3_006475

NIZ:	NNRS527_01545

ALEG:

CFBP4996_27405

MIWA:

SS37A_35500 SS37A_39100

LEJ:	ETW24_21435

THAC:

CSC3H3_23830

TLU:	R1T41_01910

BBAD:

K7T73_21425

PCAL:

BV455_03919(iucC_1)

LGY:	T479_22385

SALE:

EPH95_16130

SAU:	SA0117(sbnF)

SAV:

SAV0121

SAW:

SAHV_0120

SAH:	SaurJH1_0112

SAJ:	SaurJH9_0108

SAM:	MW0094(sbnF)

SAS:

SAS0095

SAR:

SAR0124

SAC:

SACOL0105

SAX:	USA300HOU_0132

SAA:	SAUSA300_0123

SAO:	SAOUHSC_00080

SAE:	NWMN_0065(sbnF)

SAD:

SAAV_0089

SUU:	M013TW_0111(sbnF)

SUV:	SAVC_00345

SUE:

SAOV_0068

SUJ:	SAA6159_00104(sbnF)

SUK:	SAA6008_00099

SUC:

ECTR2_77

SUT:	SAT0131_00107

SUQ:	HMPREF0772_10376

SUZ:

MS7_0113

SUD:	ST398NM01_0133

SUX:	SAEMRSA15_00870

SUW:	SATW20_01330(sbnF)

SUG:

SAPIG0133

SUF:	SARLGA251_00960(sbnF)

SAUA:

SAAG_00605

SAUE:

RSAU_000075

SAUN:

SAKOR_00095

SAUS:

SA40_0088(sbnF)

SAUU:

SA957_0103(sbnF)

SAUG:

SA268_0100(sbnF)

SAUZ:

SAZ172_0132(sbnF)

SAUT:

SAI1T1_2000770

SAUJ:

SAI2T2_1000770

SAUK:

SAI3T3_1000770

SAUQ:

SAI4T8_1000770

SAUV:

SAI7S6_1000770

SAUW:

SAI5S5_1000770

SAUX:

SAI6T6_1000770

SAUY:

SAI8T7_1000770

SAUF:

X998_0100

SAB:

SAB0060

SUY:	SA2981_0122(sbnF)

SAUB:

C248_0109

SAUM:

BN843_1230

SAUC:

CA347_132

SAUR:

SABB_03469

SAUI:

AZ30_00630

SAUD:

CH52_05115

SAMS:

NI36_00525

SUH:	SAMSHR1132_00970

SSD:	SPSINT_0339

SDT:

SPSE_2119

SDP:	NCTC12225_02336(iucC_3)

SHU:	SHYC_00380(sbnF)

SSCH:

LH95_00380

SSCZ:

RN70_00600

SAGQ:

EP23_09745(sbnF)

SEQO:

SE1039_25060

SLZ:	B5P37_07615

SARL:

SAP2_02080

SSH:	NCTC13712_00074(iucC_2)

SRAI:

LN051_00255(sbnF)

SROT:

ML435_00425(sbnF)

STAP:

AOB58_1878

SFF:	FOB90_07410(sbnF)

MBRU:

MGG13_01650

BAGR:

BA6348_15330

BHUI:

LOK74_15455

BBOR:

RFB14_00520

PPOL:

X809_04490

PTA:	HPL003_19615

PPEO:

ABE82_04645

PNP:	IJ22_24490

POW:	IJ21_46090

PXL:	BS614_08685

PKB:	B4V02_14040

PPSC:

EHS13_31655

PBAC:

HUB98_21835

PKA:	PQ456_10720

PMAH:

PTQ21_17800

PAMY:

P9222_32715

PMAE:

LMZ02_21540

PHK:	SK066_11550

PNK:	AASFL403_08170

PPAB:

KET34_14040

ANX:	ACH33_02535 ACH33_02540

ATHE:

K3F53_16415

CCHL:

FPL14_15120

SACA:

FFV09_03640 FFV09_03645

TUM:	CBW65_11375 CBW65_11410

TAB:	CIG75_02865

LFB:	C1X05_04255

KPUL:

GXN76_14230

PABS:

JIR001_05140

SACG:

FDZ84_26455

PSEQ:

AD006_29045

PECQ:

AD017_29435

PMAD:

BAY61_28590

ACTY:

OG774_04525

» show all

Reference

1 [PMID:19775248]

Authors

Cheung J, Beasley FC, Liu S, Lajoie GA, Heinrichs DE

Title

Molecular characterization of staphyloferrin B biosynthesis in Staphylococcus aureus.

Journal

Mol Microbiol 74:594-608 (2009)
DOI:10.1111/j.1365-2958.2009.06880.x

Other DBs

ExplorEnz - The Enzyme Database:

6.3.2.55

IUBMB Enzyme Nomenclature:

6.3.2.55

ExPASy - ENZYME nomenclature database:

6.3.2.55

BRENDA, the Enzyme Database:

6.3.2.55

DBGET integrated database retrieval system