KEGG ENZYME: 6.3.2.56

ENZYME: 6.3.2.56

Entry

EC 6.3.2.56                 Enzyme

Name

staphyloferrin B synthase;
sbnC (gene name)

Class

Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)

Sysname

2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate:2-oxoglutarate ligase (staphyloferrin B-forming)

Reaction(IUBMB)

ATP + 2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate + 2-oxoglutarate = AMP + diphosphate + staphyloferrin B [RN:R12312]

Reaction(KEGG)

R12312

Substrate

ATP [CPD:C00002];
2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate [CPD:C22074];
2-oxoglutarate [CPD:C00026]

Product

AMP [CPD:C00020];
diphosphate [CPD:C00013];
staphyloferrin B [CPD:C22075]

Comment

Requires Mg2+. The enzyme, characterized from the bacterium Staphylococcus aureus, catalyses the last step in the biosynthesis of the siderophore staphyloferrin B. It belongs to a class of siderophore synthases known as type B nonribosomal peptide synthase-independent synthases (NIS). Type B NIS enzymes recognize the delta-acid group of 2-oxoglutarate. The enzyme forms a 2-oxoglutarate adenylate intermediate prior to ligation.

History

EC 6.3.2.56 created 2019

Pathway

ec00997

Biosynthesis of various other secondary metabolites

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K23375

staphyloferrin B synthase

Genes

DDD:	Dda3937_02849(acsA)

DZE:	Dd1591_2672

DDC:	Dd586_2579

DZC:	W909_07430

DSO:	A4U42_16825

CED:	LH89_04350

DFN:	CVE23_08430

DDQ:

DDI_1279

DAQ:	DAQ1742_01516(acsA)

DIC:	Dpoa569_002353

DLC:	O1Q98_19095

LBQ:	CKQ53_11540

LPOP:

I6N93_10200

SGL:

SGP1_0039

XDO:

XDD1_1968

VTA:

A3468

PFV:	Psefu_2381

PSB:

Psyr_2589

PSYR:

N018_14365

PSP:	PSPPH_2756

PSAV:

PSA3335_16150

PAMG:

BKM19_016045

PAVL:

BKM03_14800

PVD:	CFBP1590__2719

PCAB:

JGS08_13325

PCOF:

POR16_12195

PTRE:

I9H09_12715

PSYI:

MME58_13600

PCG:	AXG94_01640

PMED:

E3Z27_14960

PBC:	CD58_14515

PCH:	EY04_15695

PCZ:	PCL1606_28890

PCP:	JM49_14870

PCHP:

C4K32_3261

PSEM:

TO66_16010

PFK:	PFAS1_21435

PSIL:

PMA3_09145

PFIT:

KJY40_15375

PNB:	NK667_16640

PSKU:

KUIN1_25740

PALI:

A3K91_1227

PSYP:

E5677_07275

ABY:

ABAYE2004

ABC:	ACICU_01676

ABN:

AB57_1873

ABB:	ABBFA_01824(iucC_1)

ABX:

ABK1_2134

ABZ:	ABZJ_01837

ABR:	ABTJ_02031

ABD:	ABTW07_1892

ABH:

M3Q_2028

ABAD:

ABD1_16360

ABJ:	BJAB07104_02076

ABAB:

BJAB0715_01859

ABAJ:

BJAB0868_01798

ABAZ:

P795_9030

ABK:	LX00_08325

ABAU:

IX87_19075

ABAA:

IX88_10160

ABW:	BL01_08510

ABAL:

ABLAC_24820

ACC:	BDGL_001499

ANO:	RR32_07475

ALC:	OTEC02_07350

ACAL:

BUM88_11505

ASEI:

I8T81_07360

ACD:	AOLE_07110

ACW:	A0J50_07095

ACV:	AMD27_03900

ARJ:	DOM24_08490

AGU:

AS4_16150

ADS:	FPL17_05850

AVIV:

LF296_08365

SDN:

Sden_0588

TACT:

SG35_003600

CEK:	D0B88_14040

CEG:	D0C16_05370

CCAG:

SR908_12765

HEL:

HELO_3332

HHH:	CLM76_01220

HSV:	HNO53_08410

HBH:	E4T21_15080

HPIZ:

GYM47_13040

HAA:	A5892_12590

RSO:

RSp0419

RSE:

F504_3905

RPU:	CDC45_19725

RNC:	GO999_24065

RSL:	RPSI07_mp0380

RSN:	RSPO_m01409

RSM:	CMR15_mp10432

RSY:	RSUY_34870(iucC_1)

RSG:	JK151_14175

CNC:	CNE_2c14850

CUH:	BJN34_08315

RME:

Rmet_1115

CUK:	KB879_12565

BPLA:

bpln_2g05030

MFLA:

GO485_03170

NLC:	EBAPG3_006460

NIZ:	NNRS527_01548

ALEG:

CFBP4996_27390

MNO:

Mnod_6950

MAQU:

Maq22A_c25435(rhbC)

MIWA:

SS37A_35530

THAC:

CSC3H3_23840

TLU:	R1T41_01920

PCAL:

BV455_03922(iucC_2)

LGY:	T479_22370

SALE:

EPH95_16145

SAU:	SA0114(sbnC)

SAV:

SAV0118

SAW:

SAHV_0117

SAH:	SaurJH1_0109

SAJ:	SaurJH9_0105

SAM:	MW0091(sbnC)

SAS:

SAS0092

SAR:

SAR0121

SAC:

SACOL0102

SAX:	USA300HOU_0129

SAA:	SAUSA300_0120(sbnC)

SAO:	SAOUHSC_00077

SAE:	NWMN_0062(sbnC)

SAD:

SAAV_0086

SUU:	M013TW_0108(sbnC)

SUV:	SAVC_00330

SUE:

SAOV_0065

SUJ:	SAA6159_00101(sbnC)

SUK:	SAA6008_00096(sbnC)

SUC:

ECTR2_74

SUT:	SAT0131_00104

SUQ:	HMPREF0772_10379

SUZ:

MS7_0110

SUD:	ST398NM01_0129

SUX:	SAEMRSA15_00840

SUW:	SATW20_01300(sbnC)

SUG:

SAPIG0129

SUF:	SARLGA251_00930(sbnC)

SAUA:

SAAG_00602

SAUE:

RSAU_000072(sbnC)

SAUN:

SAKOR_00092

SAUS:

SA40_0085(sbnC)

SAUU:

SA957_0100(sbnC)

SAUG:

SA268_0097(sbnC)

SAUZ:

SAZ172_0129(sbnC)

SAUT:

SAI1T1_2000740

SAUJ:

SAI2T2_1000740

SAUK:

SAI3T3_1000740

SAUQ:

SAI4T8_1000740

SAUV:

SAI7S6_1000740

SAUW:

SAI5S5_1000740

SAUX:

SAI6T6_1000740

SAUY:

SAI8T7_1000740

SAUF:

X998_0097

SAB:

SAB0057

SUY:	SA2981_0119(sbnC)

SAUB:

C248_0106

SAUM:

BN843_1200

SAUC:

CA347_129

SAUR:

SABB_01722(sbnC)

SAUI:

AZ30_00615

SAUD:

CH52_05130

SAMS:

NI36_00510

SUH:	SAMSHR1132_00940

SSD:	SPSINT_0336

SDT:

SPSE_2122

SDP:	NCTC12225_02339(iucC_4)

SHU:	SHYC_00365

SSCH:

LH95_00365

SSCZ:

RN70_00585

SAGQ:

EP23_09760(sbnC)

SEQO:

SE1039_25090

SLZ:	B5P37_07600

SARL:

SAP2_02050

SSH:	NCTC13712_00071(iucC_1)

SRAI:

LN051_00240(sbnC)

SROT:

ML435_00410(sbnC)

STAP:

AOB58_1875

SFF:	FOB90_07425(sbnC)

BAGR:

BA6348_15345

BHUI:

LOK74_15450

PPOL:

X809_04505

PTA:	HPL003_19630

PPEO:

ABE82_04660

PNP:	IJ22_24480

POW:	IJ21_46060

PXL:	BS614_08700

PKB:	B4V02_14025

PAIH:

ASL14_00100

PPSC:

EHS13_31640

PBAC:

HUB98_21855

PKA:	PQ456_10735

PAMY:

P9222_32690

PMAE:

LMZ02_21525

PHK:	SK066_11565

PNK:	AASFL403_08155

CCHL:

FPL14_15135

TUM:	CBW65_11400

TAB:	CIG75_02855

LFB:	C1X05_04240

PABS:

JIR001_05110

SALJ:

SMD11_0168

SHAU:

K9S39_08610

SACG:

FDZ84_26440

PMAD:

BAY61_28580

ACTY:

OG774_01350

» show all

Reference

1 [PMID:19775248]

Authors

Cheung J, Beasley FC, Liu S, Lajoie GA, Heinrichs DE

Title

Molecular characterization of staphyloferrin B biosynthesis in Staphylococcus aureus.

Journal

Mol Microbiol 74:594-608 (2009)
DOI:10.1111/j.1365-2958.2009.06880.x

Other DBs

ExplorEnz - The Enzyme Database:

6.3.2.56

IUBMB Enzyme Nomenclature:

6.3.2.56

ExPASy - ENZYME nomenclature database:

6.3.2.56

BRENDA, the Enzyme Database:

6.3.2.56

DBGET integrated database retrieval system