KEGG ENZYME: 6.3.2.63

ENZYME: 6.3.2.63

Entry

EC 6.3.2.63                 Enzyme

Name

putrebactin synthase;
pubC (gene name)

Class

Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)

Sysname

N1-hydroxy-N1-succinylputrescine:N1-hydroxy-N1-succinylputrescine ligase

Reaction(IUBMB)

2 ATP + 2 N1-hydroxy-N1-succinylputrescine = 2 AMP + 2 diphosphate + putrebactin (overall reaction);
(1a) ATP + 2 N1-hydroxy-N1-succinylputrescine = AMP + diphosphate + pre-putrebactin;
(1b) ATP + pre-putrebactin = AMP + diphosphate + putrebactin

Substrate

ATP [CPD:C00002];
N1-hydroxy-N1-succinylputrescine;
pre-putrebactin

Product

AMP [CPD:C00020];
diphosphate [CPD:C00013];
putrebactin;
pre-putrebactin

Comment

Requires Mg2+. The enzyme, characterized from the bacteria Shewanella spp. MR-4 and MR-7, catalyse the last step in the biosynthesis of the siderophore putrebactin. The enzyme catalyses the reaction in two steps - concatenation of two molecules of N1-hydroxy-N1-succinylputrescine, followed by cyclization.

History

EC 6.3.2.63 created 2024

Reference

1 [PMID:18630910]

Authors

Kadi N, Arbache S, Song L, Oves-Costales D, Challis GL.

Title

Identification of a gene cluster that directs putrebactin biosynthesis in Shewanella species: PubC catalyzes cyclodimerization of N-hydroxy-N-succinylputrescine.

Journal

J Am Chem Soc 130:10458-9 (2008)
DOI:10.1021/ja8027263

Other DBs

ExplorEnz - The Enzyme Database:

6.3.2.63

IUBMB Enzyme Nomenclature:

6.3.2.63

ExPASy - ENZYME nomenclature database:

6.3.2.63

BRENDA, the Enzyme Database:

6.3.2.63

All links

Chemical substance (3)   
   KEGG COMPOUND (3)   
All databases (3)   

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