KEGG ENZYME: 6.3.4.24

ENZYME: 6.3.4.24

Entry

EC 6.3.4.24                 Enzyme

Name

tyramine---L-glutamate ligase;
mfnD (gene name)

Class

Ligases;
Forming carbon-nitrogen bonds;
Other carbon-nitrogen ligases

Sysname

tyramine:L-glutamate gamma-ligase (ADP-forming)

Reaction(IUBMB)

ATP + tyramine + L-glutamate = ADP + phosphate + gamma-glutamyltyramine [RN:R10902]

Reaction(KEGG)

R10902

Substrate

ATP [CPD:C00002];
tyramine [CPD:C00483];
L-glutamate [CPD:C00025]

Product

ADP [CPD:C00008];
phosphate [CPD:C00009];
gamma-glutamyltyramine [CPD:C20926]

Comment

The enzyme, which has been characterized from the archaea Methanocaldococcus fervens, participates in the biosynthesis of the cofactor methanofuran. Requires a divalent cation for activity, with Mn2+ giving the highest activity, followed by Mg2+, Co2+, Zn2+, and Fe2+.

History

EC 6.3.4.24 created 2014

Pathway

ec00680

Methane metabolism

ec01100

Metabolic pathways

Orthology

K06914

tyramine---L-glutamate ligase

Genes

EMO:	DM558_09860

MCA:

MCA2868

METU:

GNH96_03505

MMEO:

OOT43_13990

MMT:	Metme_2270

MDN:	JT25_019360

MDH:	AYM39_08565

MKO:	MKLM6_1783

METL:

U737_14795

MPAD:

KEF85_11450

MELL:

IVG45_03955

MRP:	NM686_014165

MMOT:

QZJ86_13630

MAH:	MEALZ_2433

MBUR:

EQU24_09090

MPSY:

CEK71_02030

MMAI:

sS8_3388

MSZE:

MSZNOR_2912

MMOB:

F6R98_18225

MEIN:

methR_P1410

MOZ:	MoryE10_04100

MISZ:

MishRS11D_40180

MESL:

KKZ03_09110

MEIY:

MIN45_P1846

MCAU:

MIT9_P1446

MEJ:

Q7A_50

MEC:

Q7C_1777

MMAF:

GCM100_17880

MTHA:

VSX76_16000

NOC:

Noc_0257

NHL:

Nhal_3881

NWA:

Nwat_0355

NWR:	E3U44_04650

NTT:

TAO_1759

TSY:	THSYN_31755

NTG:	NSCAC_0079

SEDS:

AAY24_01680

COX:	E0W60_31985

BUG:	BC1001_3969

BGF:	BC1003_5433

BYI:	BYI23_B014250

BUE:	BRPE67_BCDS01220

BUQ:	AC233_22140

BPH:	Bphy_5936 Bphy_6502

BGE:	BC1002_3202

BCAI:

K788_0001566 K788_0007399

PSPW:

BJG93_19500

PARA:

BTO02_23540

PHS:	C2L64_21765

PTER:

C2L65_26230

PGP:	CUJ91_21390

PCJ:	CUJ87_23530

BUO:	BRPE64_BCDS01340

VAP:	Vapar_3069

VPD:	VAPA_1c31340

MPT:

Mpe_A2621

METP:

C1M51_15035

RGE:

RGE_40360

LCH:

Lcho_3138

MLIR:

LPB04_04550

MFLA:

GO485_25020

MPLI:

E1742_23890

ACTB:

RHM62_12615

METR:

BSY238_3386

MFA:

Mfla_1650

MMB:

Mmol_1335

MEH:

M301_1544

MEI:	Msip34_1493

MEP:

MPQ_1556

MBAC:

BN1209_0774

MEU:	ACJ67_07435

MPAU:

ZMTM_11920

SPLB:

SFPGR_25910

AZA:	AZKH_p0261

AZD:	CDA09_11455

DDZ:	DSYM_25740

XAU:

Xaut_1790

XDI:	EZH22_23940

STAR:

G3545_09800

SNO:

Snov_0746

ANC:	GBB76_00730

APRA:

G3A50_16475

APOL:

K9D25_20210

CMET:

K6K41_25455

ALI:	AZOLI_p10884

ABS:	AZOBR_p270078

ABQ:	ABAZ39_25495

ABF:	AMK58_22305

ATI:	AL072_21135

AZT:	TSH58p_01230

AZM:	DM194_22135

AOZ:	HUE56_08895

AARE:

D3093_24935

AZS:	E6C72_18220

FWA:	DCMF_03940

HAS:	Halsa_0958

RBA:

RB11969

PSL:

Psta_3591

RUL:

UC8_21010

RML:	FF011L_17930

MFF:	MFFC18_09560

ROL:	CA51_35250

RUV:

EC9_39480

RCF:	Poly24_39710

AHEL:

Q31a_40000

LCRE:

Pla8534_39490

AAGG:

ETAA8_35450

BVO:	Pan97_22160

BREM:

PSR63_00215

RLC:	K227x_21260

SMAM:

Mal15_10170

SNEP:

Enr13x_62830

LPAV:

PLANPX_2816

LLH:

I41_29940

AMUC:

Pan181_27090

PND:	Pla175_21270

AMOB:

HG15A2_27280

BMEI:

Spa11_11640

PLM:

Plim_0283

PEH:	Spb1_10390

PBS:	Plabr_4663

PLH:	VT85_05490

FMR:	Fuma_03108

GMR:	GmarT_45480

GPN:	Pan110_43600

GFM:	Enr17x_46110

MRI:	Mal4_12180

SDYN:

Mal52_29050

PLON:

Pla110_07530

ACAF:

CA12_13940

TPOL:

Mal48_45010

CCOS:

Pan44_38020

PVAR:

SH412_003621

CCOP:

Mal65_36160

GES:	VT84_16265

GOG:	C1280_17355

GMS:	SOIL9_38910

TIM:	GMBLW1_22340

LRS:	PX52LOC_05125

FTJ:

FTUN_7518

ULI:	ETAA1_22520

TSPH:

KIH39_21365

SACI:

Sinac_1217

PBOR:

BSF38_00158

AGV:	OJF2_59340

MOX:

DAMO_2657

MJA:

MJ_0815

MFE:	Mefer_1180

MVU:	Metvu_0080

MFS:	MFS40622_1347

MIF:	Metin_1143

MJH:	JH146_1087

MESG:

MLAUSG7_1214(mfnD)

MESA:

MLASG1_0563(mfnD)

MIG:	Metig_1750

MMP:

MMP0564

MMQ:	MmarC5_1043

MMX:	MmarC6_0329

MMZ:	MmarC7_1583

MMD:

GYY_03655

MMAK:

MMKA1_06120

MMAO:

MMOS7_06070

MMAD:

MMJJ_04200

MAE:

Maeo_0448

MVN:	Mevan_1440

MVO:

Mvol_0760

MOK:	Metok_1374

METF:

CFE53_01545

MTH:

MTH_835

MMG:	MTBMA_c12330

MWO:	MWSIV6_1212(mfnD)

MTEE:

MTTB_05400

METC:

MTCT_0749

METE:

tca_00802

METZ:

METMT2_0908

METK:

FVF72_07365

MTHM:

FZP57_05755

METJ:

FZP68_00450

MST:

Msp_0387

METB:

AW729_05135

MEIS:

PXD04_05745

MRU:

mru_1696

MSI:

Msm_0852

MEB:

Abm4_0504

MMIL:

sm9_1779

MEYE:

TL18_07950

MOL:

YLM1_1164

MARO:

MarbSA_18740

METV:

K4897_07220

MEL:	Metbo_1949

MEW:	MSWAN_0670

METH:

MBMB1_0690

MFC:

BRM9_1505

MFI:	DSM1535_2199

MCUB:

MCBB_0701(mfnD)

MSUB:

BK009_01670

METN:

BK008_09255

METT:

CIT01_01990

METO:

CIT02_03320

MEME:

HYG87_03505

MFEG:

GCM10025860_17600

MFV:

Mfer_0210

MKA:

MK0114

AFU:

AF_1061

AFG:	AFULGI_00011600

AVE:	Arcve_1368

AST:	Asulf_01700

FPL:

Ferp_2285

GAC:

GACE_0439

GAH:

GAH_01280

MBA:	Mbar_A3436

MBY:	MSBRM_1199

MBW:	MSBRW_1297

MBAR:

MSBR2_1211

MBAK:

MSBR3_1316

MAC:

MA_1475

MMA:

MM_2510

MMAZ:

MmTuc01_2559

MMJ:	MSMAS_2193

MMAC:

MSMAC_2261

MVC:	MSVAZ_1446

MEK:	MSKOL_1391

MLS:	MSLAZ_0912

METM:

MSMTP_0947

MEF:	MSWH1_1890

MEQ:	MSWHS_3183

MSJ:	MSSAC_1475

MSZ:	MSSIH_1484

MSW:	MSSIT_1540

MTHR:

MSTHT_0225

MTHE:

MSTHC_0507

MHOR:

MSHOH_2802

MFZ:	AOB57_001295

MBU:

Mbur_1315

MMET:

MCMEM_1762

MELO:

J7W08_07840

MMH:

Mmah_1619

MHAZ:

BHR79_05630

MPOT:

BKM01_01720

MEV:	Metev_0255

MZH:	Mzhil_1619

MPY:

Mpsy_2546

MZI:	HWN40_09635

MMAV:

RE476_00405

MSEB:

RE474_10150

MHZ:	Metho_0940

MTP:

Mthe_1641

MCJ:

MCON_1686

MHI:

Mhar_1058

MHU:

Mhun_0571

MRTJ:

KHC33_08690

MLA:

Mlab_0268

MEM:	Memar_1384

MBG:	BN140_1324

MEMA:

MMAB1_1685

MCHK:

MchiMG62_15810

MSUM:

OH143_01145

MRC:	R6Y96_05785

MPI:

Mpet_1053

MEFW:

F1737_05575

MAQE:

RJ40_03470

MFK:	E2N92_03525

MOU:	OU421_01765

MESB:

L1S32_05280

MANQ:

L1994_06370

MBN:

Mboo_0920

MFO:	Metfor_1879

MPL:

Mpal_0581

MPD:

MCP_1324

MEZ:

Mtc_1029

RCI:

RCIX2220

MELU:

MTLP_02150

MEUZ:

KRP56_00755

BARB:

AOA66_0934(mptN)

LOKI:

Lokiarch_26760(ddl)

» show all

Reference

1 [PMID:25211225]

Authors

Wang Y, Xu H, Harich KC, White RH

Title

Identification and characterization of a tyramine-glutamate ligase (MfnD) involved in methanofuran biosynthesis.

Journal

Biochemistry 53:6220-30 (2014)
DOI:10.1021/bi500879h

Sequence

[mja:MJ_0815] [mfe:Mefer_1180]

Other DBs

ExplorEnz - The Enzyme Database:

6.3.4.24

IUBMB Enzyme Nomenclature:

6.3.4.24

ExPASy - ENZYME nomenclature database:

6.3.4.24

BRENDA, the Enzyme Database:

6.3.4.24

DBGET integrated database retrieval system