KEGG ENZYME: 6.5.1.5

ENZYME: 6.5.1.5

Entry

EC 6.5.1.5                  Enzyme

Name

RNA 3'-terminal-phosphate cyclase (GTP);
Pf-Rtc;
RNA-3'-phosphate cyclase (GTP)

Class

Ligases;
Forming phosphoric-ester bonds;
Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)

Sysname

RNA-3'-phosphate:RNA ligase (cyclizing, GMP-forming)

Reaction(IUBMB)

GTP + [RNA]-3'-(3'-phospho-ribonucleoside) = GMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside (overall reaction) [RN:R10537];
(1a) GTP + [RNA 3'-phosphate cyclase]-L-histidine = 5'-guanosyl [RNA 3'-phosphate cyclase]-Ntau-phosphono-L-histidine + diphosphate;
(1b) 5'-guanosyl [RNA 3'-phosphate cyclase]-Ntau-phosphono-L-histidine + [RNA]-3'-(3'-phospho-ribonucleoside) = [RNA 3'-phosphate cyclase]-L-histidine + [RNA]-3'-ribonucleoside-3'-(5'-diphosphoguanosine);
(1c) [RNA]-3'-ribonucleoside-3'-(5'-diphosphoguanosine) = [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside + GMP

Reaction(KEGG)

R10537

Substrate

GTP [CPD:C00044];
[RNA]-3'-(3'-phospho-ribonucleoside);
[RNA 3'-phosphate cyclase]-L-histidine;
5'-guanosyl [RNA 3'-phosphate cyclase]-Ntau-phosphono-L-histidine;
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoguanosine)

Product

GMP [CPD:C00144];
diphosphate [CPD:C00013];
[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside;
5'-guanosyl [RNA 3'-phosphate cyclase]-Ntau-phosphono-L-histidine;
[RNA 3'-phosphate cyclase]-L-histidine;
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoguanosine)

Comment

The enzyme, which is specific for GTP, was characterized from the archaeon Pyrococcus furiosus. The enzyme converts the 3'-terminal phosphate of various RNA substrates into the 2',3'-cyclic phosphodiester in a GTP-dependent reaction. Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by GTP, forming a phosphoramide bond between guanylate and a histidine residue. The guanylate group is then transferred to the 3'-phosphate terminus of the substrate, forming the capped structure [RNA]-3'-(5'-diphosphoguanosine). Finally, the enzyme catalyses an attack of the vicinal O-2' on the 3'-phosphorus, which results in formation of cyclic phosphate and release of the guanylate. cf. EC 6.5.1.4, RNA-3'-phosphate cyclase (ATP).

History

EC 6.5.1.5 created 2013, modified 2016

Orthology

K18105

RNA 3'-terminal phosphate cyclase (GTP)

Genes

TMAI:

FVE67_06470(rtcA)

THUG:

KNN16_13000(rtcA)

NPY:	NPRO_05470

NTI:	DNFV4_01817

MTHM:

FZP57_02120

MKA:	MK0001(rcl1)

PFU:

PF1549

PFI:

PFC_06975

PHO:	PH1529(PH1529)

PAB:

PAB1943

PYN:

PNA2_0113

PYA:	PYCH_05910

PYS:

Py04_1420

PYC:	TQ32_09235

TKO:

TK1615

TON:

TON_0048

TGA:	TGAM_2020(rtcA)

TSI:

TSIB_1528

TBA:	TERMP_00074

THE:

GQS_05015

THA:

TAM4_6

THM:

CL1_0647

TLT:

OCC_03117

THS:

TES1_0173

TNU:

BD01_2074

TEU:

TEU_04715

TGY:	X802_03300

THV:	ADU37_CDS16220

TCH:	CHITON_1756

TPEP:

A0127_07130

TPIE:

A7C91_06170

TGG:	A3K92_00565

TCE:	A3L02_00995

TBS:	A3L01_00895

THH:	CDI07_00890

TSL:	A3L11_04640

TTD:	A3L14_02295

TPRF:

A3L09_02395

TRL:	A3L10_05680

TPAF:

A3L08_03950

THY:	A3L12_00545

TIC:	FH039_02715

TCQ:	TIRI35C_0394(rtcA)

THEM:

FPV09_06605

THEI:

K1720_00305

PPAC:

PAP_08005

MPOT:

BKM01_04350

MEAM:

MU439_01765(rtcA)

MEAE:

QEN48_00595(rtcA)

THEL:

IG193_06520

» show all

Reference

1 [PMID:22074260]

Authors

Sato A, Soga T, Igarashi K, Takesue K, Tomita M, Kanai A

Title

GTP-dependent RNA 3'-terminal phosphate cyclase from the hyperthermophilic archaeon Pyrococcus furiosus.

Journal

Genes Cells 16:1190-9 (2011)
DOI:10.1111/j.1365-2443.2011.01561.x

Sequence

[pfu:PF1549]

Other DBs

ExplorEnz - The Enzyme Database:

6.5.1.5

IUBMB Enzyme Nomenclature:

6.5.1.5

ExPASy - ENZYME nomenclature database:

6.5.1.5

BRENDA, the Enzyme Database:

6.5.1.5

All links

Chemical substance (5)   
   KEGG COMPOUND (5)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (51)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (50)   
Protein sequence (3)   
   UniProt (2)   
   SWISS-PROT (1)   
DNA sequence (16)   
   GenBank (8)   
   EMBL (8)   
Protein domain (8)   
   InterPro (8)   
All databases (85)   

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