KEGG   ENZYME: 6.7.1.1
Entry
EC 6.7.1.1                  Enzyme                                 
Name
3-amino-2-hydroxy-4-methoxybenzoate diazotase;
creM (gene name)
Class
Ligases;
Forming nitrogen-nitrogen bonds;
Forming diazo bonds
Sysname
3-amino-2-hydroxy-4-methoxybenzoate:nitrite ligase (AMP-forming)
Reaction(IUBMB)
ATP + 3-amino-2-hydroxy-4-methoxybenzoate + nitrite = AMP + diphosphate + cremeomycin + H2O [RN:R12943]
Reaction(KEGG)
R12943
Substrate
ATP [CPD:C00002];
3-amino-2-hydroxy-4-methoxybenzoate [CPD:C22425];
nitrite [CPD:C00088]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
cremeomycin [CPD:C22416];
H2O [CPD:C00001]
Comment
The enzyme, characterized from Streptomyces cremeus, catalyses the last step in the biosynthesis of the ortho-diazoquinone cremeomycin.
History
EC 6.7.1.1 created 2021
Pathway
ec00998  Biosynthesis of various antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K25598  3-amino-2-hydroxy-4-methoxybenzoate diazotase
Reference
1  [PMID:29771512]
  Authors
Waldman AJ, Balskus EP.
  Title
Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis.
  Journal
J Org Chem 83:7539-7546 (2018)
DOI:10.1021/acs.joc.8b00367
Other DBs
ExplorEnz - The Enzyme Database: 6.7.1.1
IUBMB Enzyme Nomenclature: 6.7.1.1
ExPASy - ENZYME nomenclature database: 6.7.1.1
BRENDA, the Enzyme Database: 6.7.1.1

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