Entry
Name
paromamine 6'-oxidase;
btrQ (gene name);
neoG (gene name);
kanI (gene name);
tacB (gene name);
neoQ (obsolete gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
paromamine:oxygen 6'-oxidoreductase
Reaction(IUBMB)
paromamine + O2 = 6'-dehydroparomamine + H2O2 [RN:
R08896 ]
Reaction(KEGG)
Substrate
Product
Comment
Contains FAD. Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. Works in combination with EC
2.6.1.93 , neamine transaminase, to replace the 6'-hydroxy group of paromamine with an amino group. The enzyme from the bacterium Streptomyces fradiae also catalyses EC
1.1.3.44 , 6'''-hydroxyneomycin C oxidase.
History
EC 1.1.3.43 created 2012
Pathway
ec00524 Neomycin, kanamycin and gentamicin biosynthesis
ec01110 Biosynthesis of secondary metabolites
Orthology
K20565 paromamine 6'-oxidase / 6'''-hydroxyneomycin C oxidase
K20566 paromamine 6'-oxidase / 2'-deamino-2'-hydroxyparomamine 6'-oxidase
K20577 paromamine/lividamine 6'-oxidase
Genes
Taxonomy
Reference
Authors
Huang F, Spiteller D, Koorbanally NA, Li Y, Llewellyn NM, Spencer JB
Title
Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin.
Journal
Reference
Authors
Yu Y, Hou X, Ni X, Xia H
Title
Biosynthesis of 3'-deoxy-carbamoylkanamycin C in a Streptomyces tenebrarius mutant strain by tacB gene disruption.
Journal
Reference
Authors
Clausnitzer D, Piepersberg W, Wehmeier UF
Title
The oxidoreductases LivQ and NeoQ are responsible for the different 6'-modifications in the aminoglycosides lividomycin and neomycin.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 1.1.3.43
ExPASy - ENZYME nomenclature database: 1.1.3.43