Entry
Name
alcohol dehydrogenase (quinone);
type III ADH;
membrane associated quinohaemoprotein alcohol dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With a quinone or similar compound as acceptor
BRITE hierarchy
Sysname
alcohol:quinone oxidoreductase
Reaction(IUBMB)
ethanol + ubiquinone = acetaldehyde + ubiquinol [RN:
R09479 ]
Reaction(KEGG)
Substrate
Product
Comment
Only described in acetic acid bacteria where it is involved in acetic acid production. Associated with membrane. Electron acceptor is membrane ubiquinone. A model structure suggests that, like all other quinoprotein alcohol dehydrogenases, the catalytic subunit has an 8-bladed 'propeller' structure, a calcium ion bound to the PQQ in the active site and an unusual disulfide ring structure in close proximity to the PQQ; the catalytic subunit also has a heme c in the C-terminal domain. The enzyme has two additional subunits, one of which contains three molecules of heme c. It does not require amines for activation. It has a restricted substrate specificity, oxidizing a few primary alcohols (C2 to C6), but not methanol, secondary alcohols and some aldehydes. It is assayed with phenazine methosulfate or with ferricyanide.
History
EC 1.1.5.5 created 2009, modified 2010
Pathway
ec00010 Glycolysis / Gluconeogenesis
ec01110 Biosynthesis of secondary metabolites
ec01120 Microbial metabolism in diverse environments
Orthology
K22473 alcohol dehydrogenase (quinone), dehydrogenase subunit
K22474 alcohol dehydrogenase (quinone), cytochrome c subunit
Genes
PGE : LG71_00075 LG71_14865 LG71_25665
SMAF : D781_1965 D781_2566
SRZ : AXX16_2551 AXX16_3163
SERM : CLM71_09740 CLM71_13325
SOF : NCTC11214_01213(adhB_1) NCTC11214_04703
SRHZ : FO014_20345 FO014_23665
RBAD : H2866_04430 H2866_04910
EGE : EM595_3151 EM595_p0028
PAO : Pat9b_0349 Pat9b_4308
PPHO : CTZ24_01860 CTZ24_26465
TCI : A7K98_08205 A7K98_12295 A7K98_17760
PFQ : QQ39_14370 QQ39_15150
FAU : Fraau_0059 Fraau_1226 Fraau_2339 Fraau_2340
PPU : PP_3382 PP_3623(adhB)
PPG : PputGB1_2294 PputGB1_2563
PPW : PputW619_2566 PputW619_2835
PPB : PPUBIRD1_2165 PPUBIRD1_2399
PPUH : B479_14215 B479_15465
PPUT : L483_13845 L483_18970
PPUN : PP4_22070 PP4_27710(gadA)
PPUD : DW66_3117 DW66_3382
PMON : X969_13685 X969_14950
PMOT : X970_13330 X970_14595
PPJ : RK21_01529 RK21_01803
PIX : RIN61_00805 RIN61_26215
PMOS : O165_005210 O165_011450
PFS : PFLU_0053(adhB) PFLU_2270
PFC : PflA506_0052 PflA506_2212
PPZ : H045_06715 H045_21375
PTV : AA957_03255 AA957_14170
PAZO : AYR47_07340 AYR47_18295
POI : BOP93_00260 BOP93_09875
PRX : HRH33_01160 HRH33_11135
PTOL : I7845_00270 I7845_11015
PFW : PF1751_v1c00530 PF1751_v1c20380
PFF : PFLUOLIPICF707980 PFLUOLIPICF726525
PFX : A7318_09900 A7318_27655
PSK : U771_00480 U771_11995
PCH : EY04_14915 EY04_29210
PCP : JM49_15430 JM49_30275
PSEC : CCOS191_2353 CCOS191_3428(adhB)
PANR : A7J50_0054 A7J50_2233
PYM : AK972_3915 AK972_5878
PGY : AWU82_05995 AWU82_14805
PEZ : HWQ56_00270 HWQ56_15125 HWQ56_15130
PVW : HU752_013880 HU752_021720
PTRT : HU722_0001275 HU722_0011680
PSAM : HU731_016335 HU731_027995
PPRG : HU725_011055 HU725_011860
PASG : KSS96_01135 KSS96_12200
PJU : L1P09_12190 L1P09_14335
PPEG : KUA23_00265 KUA23_18570
PASI : LG197_19910 LG197_21150
PKM : PZ739_14650 PZ739_16010
PTAI : ICN73_19005 ICN73_22120
PKK : QQ992_11095 QQ992_12405
PPAE : LDL65_11405 LDL65_21745
PSJY : AA098_15220 AA098_16800
NWE : SAMEA3174300_0545(adhB_1)
NZO : SAMEA4504057_1735(adhB_2)
NCI : NCTC10296_01014(adhB_1)
NANI : NCTC12227_01125(adhB_1)
CUP : BKK80_11100 BKK80_20455
BVI : Bcep1808_4795 Bcep1808_4962 Bcep1808_5830
BVE : AK36_3961 AK36_4124 AK36_5314
BUR : Bcep18194_B1601 Bcep18194_B1792
BCM : Bcenmc03_3292 Bcenmc03_5084 Bcenmc03_5949 Bcenmc03_6144
BCH : Bcen2424_4225 Bcen2424_4408 Bcen2424_5193 Bcen2424_6536
BCJ : BCAM1358 BCAM1541 BCAS0491
BCEN : DM39_3470 DM39_3646 DM39_7090(adhB)
BCEW : DM40_4249 DM40_4395 DM40_5112(adhB) DM40_5126(adhB)
BCEO : I35_5210 I35_5395 I35_7535
BAM : Bamb_3647 Bamb_3829 Bamb_6575
BAC : BamMC406_4121 BamMC406_4297 BamMC406_6289
BMJ : BMULJ_04126(cccA) BMULJ_04290(cccA)
BMU : Bmul_4216 Bmul_4380 Bmul_5499
BMK : DM80_3371 DM80_3390 DM80_3544 DM80_5719(adhB) DM80_5947
BMUL : NP80_4252 NP80_4400 NP80_5569
BCED : DM42_3547 DM42_3729 DM42_7055
BCEP : APZ15_17875 APZ15_31720
BDL : AK34_3985 AK34_4148 AK34_5710
BPYR : ABD05_28480 ABD05_29405
BCON : NL30_04770 NL30_05860
BDF : WI26_19795 WI26_20600 WI26_27690
BLAT : WK25_16240 WK25_16310 WK25_23605 WK25_24340
BTEI : WS51_04490 WS51_05255 WS51_27275 WS51_27345
BSEM : WJ12_16735 WJ12_26595 WJ12_27500
BPSL : WS57_06465 WS57_07300 WS57_15830
BMEC : WJ16_17090 WJ16_25190 WJ16_26050
BSTG : WT74_25560 WT74_27005
BSTL : BBJ41_19830 BBJ41_20765
BANN : JFN94_23930 JFN94_24705
BARI : NLX30_30810 NLX30_31810
BAEN : L3V59_19915 L3V59_20910
BGL : bglu_1g14860 bglu_2g03210
BUG : BC1001_4011 BC1001_5383 BC1001_5671
BGF : BC1003_4039 BC1003_5034 BC1003_5143 BC1003_5770
BGD : bgla_2g04730 bgla_2g10420 bgla_2g20210
BUQ : AC233_23160 AC233_30770
BGP : BGL_2c03700 BGL_2c09150
BPLA : bpln_2g04160 bpln_2g10050
BURK : DM992_18080 DM992_19145
BPH : Bphy_3250 Bphy_4796 Bphy_6799
BGE : BC1002_3299 BC1002_3760 BC1002_5566
BPX : BUPH_00591 BUPH_05700 BUPH_06089
BPY : Bphyt_0226 Bphyt_2030 Bphyt_4638 Bphyt_4961
BUZ : AYM40_24795 AYM40_29335
BFN : OI25_4124 OI25_4867 OI25_6696
BCAI : K788_0000450 K788_0002770 K788_0004716
PSPW : BJG93_16805 BJG93_19125
PARA : BTO02_08210 BTO02_30925
PARB : CJU94_21100 CJU94_24310
PHS : C2L64_26685 C2L64_45745
PGP : CUJ91_20385 CUJ91_25435
PCJ : CUJ87_18300 CUJ87_19865
PTS : CUJ90_09110 CUJ90_09435 CUJ90_32240
PTRO : G5S35_18005 G5S35_18630
PACP : FAZ97_16870 FAZ97_25705 FAZ97_32525
PACS : FAZ98_14750 FAZ98_16565 FAZ98_21655 FAZ98_24025 FAZ98_35045
PEW : KZJ38_15045 KZJ38_33025
PBRY : NDK50_25935 NDK50_29805
PPAI : E1956_21595 E1956_22730
CABA : SBC2_65710 SBC2_71690 SBC2_75680
BAV : BAV1805(adhB) BAV3247
BTRM : SAMEA390648701021 SAMEA390648703345(adhB)
BGV : CAL12_14280 CAL12_21190
AXY : AXYL_03687 AXYL_05681(adhB)
AXO : NH44784_028741 NH44784_052221
AXN : AX27061_3357 AX27061_5442
AXX : ERS451415_03338 ERS451415_05468(adhB_2)
ADT : APT56_16545 APT56_25045
AAEG : RA224_04310 RA224_14860
HSE : Hsero_3341 Hsero_4480
HSZ : ACP92_16735 ACP92_22370
HHF : E2K99_17340 E2K99_23570 E2K99_23630
HFR : G5S34_15375 G5S34_15390 G5S34_18430
DUG : HH213_12005 HH213_17355
DZO : SR858_00240 SR858_25630
DAA : AKL17_3163 AKL17_3963
GOX : GOX1067 GOX1068 GOX1230
GOH : B932_0901 B932_1062 B932_1063
GOY : GLS_c05020(adhB1) GLS_c11360(adhB3) GLS_c11370(adhA) GLS_c13170(adhB4)
GAL : A0U94_12970 A0U94_12975 A0U94_13705
GTI : FXF46_05750 FXF46_05755 FXF46_06495
GSP : IGS75_00740 IGS75_05995 IGS75_06000 IGS75_07940
GFA : MKW11_05715 MKW11_05720 MKW11_06445 MKW11_12070
GDI : GDI0856 GDI2040(adhA) GDI2041(adhB)
GDJ : Gdia_0262 Gdia_0263 Gdia_1163
GXY : GLX_11300 GLX_26600 GLX_26610
GXL : H845_1126 H845_2297 H845_603 H845_604
KNA : B0W47_05395 B0W47_13405 B0W47_13410
KEU : S101446_00260(exaA) S101446_01588 S101446_02119 S101446_02992 S101446_02993
KSC : CD178_00411(adhA) CD178_00412(adhB_1) CD178_01765(adhB_2)
KRE : GWK63_03945 GWK63_03950 GWK63_11530
KOI : LV478_08960 LV478_08965 LV478_18275
APT : APA01_00860 APA01_00870
APW : APA42C_00860 APA42C_00870
APF : APA03_00860 APA03_00870
APU : APA07_00860 APA07_00870
APG : APA12_00860 APA12_00870
APQ : APA22_00860 APA22_00870
APX : APA26_00860 APA26_00870
APZ : APA32_00860 APA32_00870
APK : APA386B_1574(adhB) APA386B_1575(adhA)
ASZ : ASN_1383(adhA) ASN_1513(adhA) ASN_1514(adhB) ASN_2761 ASN_3435
ASV : WG31_00425 WG31_00430
AACE : A0U92_12090 A0U92_12095 A0U92_13970 A0U92_15155
APER : A0U91_08220 A0U91_08225
APOM : CPF11_06905 CPF11_06910
ATO : CIW82_04750 CIW82_04755 CIW82_05150 CIW82_17535
AASC : A4S02_12635 A4S02_12640
ACET : DS739_00515 DS739_00520
AOT : AcetOri_orf03228 AcetOri_orf03230 AcetOri_orf03917
AOY : EOV40_000655 EOV40_000660
ACEK : FLP30_03845 FLP30_03850 FLP30_08750
KBA : A0U89_01190 A0U89_01195 A0U89_11770
NCH : A0U93_00180 A0U93_06965 A0U93_06970 A0U93_08050
SSAM : E3D00_02240 E3D00_02245 E3D00_03725
KHA : IFJ82_04780 IFJ82_09150 IFJ82_12150 IFJ82_12155
NGU : QN315_01335 QN315_01340 QN315_15895
PARH : I5S86_13780 I5S86_15640
» show all
Taxonomy
Reference
Authors
Gomez-Manzo S, Contreras-Zentella M, Gonzalez-Valdez A, Sosa-Torres M, Arreguin-Espinoza R, Escamilla-Marvan E
Title
The PQQ-alcohol dehydrogenase of Gluconacetobacter diazotrophicus.
Journal
Reference
Authors
Shinagawa E, Toyama H, Matsushita K, Tuitemwong P, Theeragool G, Adachi O
Title
A novel type of formaldehyde-oxidizing enzyme from the membrane of Acetobacter sp. SKU 14.
Journal
Reference
Authors
Chinnawirotpisan P, Theeragool G, Limtong S, Toyama H, Adachi OO, Matsushita K
Title
Quinoprotein alcohol dehydrogenase is involved in catabolic acetate production, while NAD-dependent alcohol dehydrogenase in ethanol assimilation in Acetobacter pasteurianus SKU1108.
Journal
Reference
Authors
Frebortova J, Matsushita K, Arata H, Adachi O
Title
Intramolecular electron transport in quinoprotein alcohol dehydrogenase of Acetobacter methanolicus: a redox-titration study
Journal
Reference
Authors
Matsushita K, Kobayashi Y, Mizuguchi M, Toyama H, Adachi O, Sakamoto K, Miyoshi H
Title
A tightly bound quinone functions in the ubiquinone reaction sites of quinoprotein alcohol dehydrogenase of an acetic acid bacterium, Gluconobacter suboxydans.
Journal
Reference
Authors
Matsushita K, Yakushi T, Toyama H, Shinagawa E, Adachi O
Title
Function of multiple heme c moieties in intramolecular electron transport and ubiquinone reduction in the quinohemoprotein alcohol dehydrogenase-cytochrome c complex of Gluconobacter suboxydans.
Journal
Reference
7
Authors
Matsushita, K., Takaki, Y., Shinagawa, E., Ameyama, M. and Adachi, O.
Title
Ethanol oxidase respiratory chain of acetic acid bacteria. Reactivity with ubiquinone of pyrroloquinolinequinone-dependent alcohol dehydrogenases purified from Acetobacter aceti and Gluconobacter suboxydans.
Journal
Biosci Biotechnol Biochem 56:304-310 (1992)
Reference
Authors
Matsushita K, Toyama H, Adachi O
Title
Respiratory chains and bioenergetics of acetic acid bacteria.
Journal
Reference
Authors
Cozier GE, Giles IG, Anthony C
Title
The structure of the quinoprotein alcohol dehydrogenase of Acetobacter aceti modelled on that of methanol dehydrogenase from Methylobacterium extorquens.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 1.1.5.5
ExPASy - ENZYME nomenclature database: 1.1.5.5
BRENDA, the Enzyme Database: 1.1.5.5