KEGG ENZYME: 1.11.1.16

ENZYME: 1.11.1.16

Entry

EC 1.11.1.16                Enzyme

Name

versatile peroxidase;
VP;
hybrid peroxidase;
polyvalent peroxidase;
reactive-black-5:hydrogen-peroxide oxidoreductase

Class

Oxidoreductases;
Acting on a peroxide as acceptor;
Peroxidases

Sysname

1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol:hydrogen-peroxide oxidoreductase

Reaction(IUBMB)

(1) 1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H2O2 = 4-hydroxy-3-methoxybenzaldehyde + 2-methoxyphenol + glycolaldehyde + H2O [RN:R11503];
(2) 2 manganese(II) + 2 H+ + H2O2 = 2 manganese(III) + 2 H2O [RN:R00011]

Reaction(KEGG)

R00011 R11503;
(other) R07612

Substrate

1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol [CPD:C21422];
H2O2 [CPD:C00027];
manganese [CPD:C00034];
H+ [CPD:C00080]

Product

4-hydroxy-3-methoxybenzaldehyde [CPD:C00755];
2-methoxyphenol [CPD:C01502];
glycolaldehyde [CPD:C00266];
H2O [CPD:C00001];
manganese [CPD:C00034]

Comment

A hemoprotein. This ligninolytic peroxidase combines the substrate-specificity characteristics of the two other ligninolytic peroxidases, EC 1.11.1.13, manganese peroxidase and EC 1.11.1.14, lignin peroxidase. Unlike these two enzymes, it is also able to oxidize phenols, hydroquinones and both low- and high-redox-potential dyes, due to a hybrid molecular architecture that involves multiple binding sites for substrates [2,4].

History

EC 1.11.1.16 created 2006, modified 2016

Reference

1 [PMID:8647081]

Authors

Martinez MJ, Ruiz-Duenas FJ, Guillen F, Martinez AT.

Title

Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii.

Journal

Eur J Biochem 237:424-32 (1996)
DOI:10.1111/j.1432-1033.1996.0424k.x

Reference

2 [PMID:9654123]

Authors

Heinfling A, Ruiz-Duenas FJ, Martinez MJ, Bergbauer M, Szewzyk U, Martinez AT.

Title

A study on reducing substrates of manganese-oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta.

Journal

FEBS Lett 428:141-6 (1998)
DOI:10.1016/S0014-5793(98)00512-2

Reference

3 [PMID:9987124]

Authors

Ruiz-Duenas FJ, Martinez MJ, Martinez AT

Title

Molecular characterization of a novel peroxidase isolated from the ligninolytic fungus Pleurotus eryngii.

Journal

Mol Microbiol 31:223-35 (1999)
DOI:10.1046/j.1365-2958.1999.01164.x

Reference

4 [PMID:10187820]

Authors

Camarero S, Sarkar S, Ruiz-Duenas FJ, Martinez MJ, Martinez AT

Title

Description of a versatile peroxidase involved in the natural degradation of lignin that has both manganese peroxidase and lignin peroxidase substrate interaction sites.

Journal

J Biol Chem 274:10324-30 (1999)
DOI:10.1074/jbc.274.15.10324

Sequence

[ag:AAD54310]

Reference

5 [PMID:10508113]

Authors

Ruiz-Duenas FJ, Martinez MJ, Martinez AT.

Title

Heterologous expression of Pleurotus eryngii peroxidase confirms its ability to oxidize Mn(2+) and different aromatic substrates.

Journal

Appl Environ Microbiol 65:4705-7 (1999)
DOI:10.1128/AEM.65.10.4705-4707.1999

Reference

6 [PMID:11004397]

Authors

Camarero S, Ruiz-Duenas FJ, Sarkar S, Martinez MJ, Martinez AT

Title

The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases.

Journal

FEMS Microbiol Lett 191:37-43 (2000)
DOI:10.1111/j.1574-6968.2000.tb09316.x

Sequence

[ag:AAD54310]

Reference

7 [PMID:11356138]

Authors

Ruiz-Duenas FJ, Camarero S, Perez-Boada M, Martinez MJ, Martinez AT.

Title

A new versatile peroxidase from Pleurotus.

Journal

Biochem Soc Trans 29:116-22 (2001)
DOI:10.1042/0300-5127:0290116

Reference

8 [PMID:12884090]

Authors

Banci L, Camarero S, Martinez AT, Martinez MJ, Perez-Boada M, Pierattelli R, Ruiz-Duenas FJ

Title

NMR study of manganese(II) binding by a new versatile peroxidase from the white-rot fungus Pleurotus eryngii.

Journal

J Biol Inorg Chem 8:751-60 (2003)
DOI:10.1007/s00775-003-0476-1

Reference

9 [PMID:16246366]

Authors

Perez-Boada M, Ruiz-Duenas FJ, Pogni R, Basosi R, Choinowski T, Martinez MJ, Piontek K, Martinez AT.

Title

Versatile peroxidase oxidation of high redox potential aromatic compounds: site-directed mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways.

Journal

J Mol Biol 354:385-402 (2005)
DOI:10.1016/j.jmb.2005.09.047

Reference

10 [PMID:10049842]

Authors

Caramelo L, Martinez MJ, Martinez AT.

Title

A search for ligninolytic peroxidases in the fungus pleurotus eryngii involving alpha-keto-gamma-thiomethylbutyric acid and lignin model dimers

Journal

Appl Environ Microbiol 65:916-22 (1999)
DOI:10.1128/AEM.65.3.916-922.1999

Other DBs

ExplorEnz - The Enzyme Database:

1.11.1.16

IUBMB Enzyme Nomenclature:

1.11.1.16

ExPASy - ENZYME nomenclature database:

1.11.1.16

BRENDA, the Enzyme Database:

1.11.1.16

CAS:	42613-30-9 114995-15-2

All links

Chemical substance (11)   
   KEGG COMPOUND (11)   
Chemical reaction (5)   
   KEGG REACTION (3)   
   KEGG RCLASS (2)   
Protein sequence (59)   
   UniProt (27)   
   SWISS-PROT (4)   
   PDBSTR (28)   
DNA sequence (49)   
   GenBank (25)   
   EMBL (24)   
3D Structure (25)   
   PDB (25)   
Protein domain (10)   
   InterPro (10)   
All databases (159)   

Download RDF

DBGET integrated database retrieval system