KEGG ENZYME: 1.13.11.59

ENZYME: 1.13.11.59

Entry

EC 1.13.11.59               Enzyme

Name

torulene dioxygenase;
CAO-2;
CarT

Class

Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen

Sysname

torulene:oxygen oxidoreductase

Reaction(IUBMB)

torulene + O2 = 4'-apo-beta,psi-caroten-4'-al + 3-methylbut-2-enal [RN:R09782]

Reaction(KEGG)

R09782

Substrate

torulene [CPD:C08613];
O2 [CPD:C00007]

Product

4'-apo-beta,psi-caroten-4'-al [CPD:C19892];
3-methylbut-2-enal [CPD:C07330]

Comment

It is assumed that 3-methylbut-2-enal is formed. The enzyme cannot cleave the saturated 3',4'-bond of gamma-carotene which implies that a 3',4'-double bond is neccessary for this reaction.

History

EC 1.13.11.59 created 2011

Pathway

ec00906

Carotenoid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K17842

torulene dioxygenase

Genes

CCP:	CHC_T00003476001 CHC_T00004955001

YLI:	YALI0C18029g

NCR:	NCU11424(cao-2)

NTE:	NEUTE1DRAFT147619(NEUTE1DRAFT_147619)

SMP:	SMAC_08627

PAN:	PODANSg626

PBEL:

QC761_400280

PPSD:

QC762_400280

PPSP:

QC763_400280

PPSA:

QC764_400280

TTT:	THITE_2122267

MGR:

MGG_08016

PPEI:

PpBr36_01466

PGRI:

PgNI_03557

SSCK:

SPSK_06058

FGR:	FGSG_02625

FPU:	FPSE_11900

FPOA:

FPOAC1_002752

FVN:	FVRRES_03062

FVR:	FVEG_00042 FVEG_09251

FOX:	FOXG_10608

NHE:	NECHADRAFT_44844 NECHADRAFT_94642

FFC:	NCS54_00854600 NCS54_00909500

FKR:	NCS57_00898300 NCS57_00927700

FMU:	J7337_005153

TRE:	TRIREDRAFT_70646

TRR:	M419DRAFT_92905

MAW:

MAC_04503

MAJ:

MAA_07825

PCHM:

VFPPC_10722

CMT:

CCM_06728

AMUS:

LMH87_004016 LMH87_010845 LMH87_011968

PLJ:	VFPFJ_09683

PTKZ:

JDV02_002040

VAL:	VDBG_07602

VDA:	VDAG_06707 VDAG_09324

CFJ:	CFIO01_00407

CLUP:

CLUP02_17262

CHIG:

CH63R_09466

SAPO:

SAPIO_CDS8504

ELA:	UCREL1_8011

PFY:	PFICI_05578 PFICI_13151

PSCO:

LY89DRAFT_687409 LY89DRAFT_707335

ANI:	ANIA_01704

AFM:	AFUA_1G17340 AFUA_2G14410

ACT:	ACLA_073070

NFI:	NFIA_008020 NFIA_089590

AOR:	AO090001000359 AO090023000740

ANG:	An04g02710

AFV:	AFLA_007082 AFLA_008701

ALUC:

AKAW2_51298A

APUU:

APUU_21415S APUU_80923S

PCS:	N7525_001617

CIM:	CIMG_03017

CPW:	CPC735_000140

PBL:	PAAG_08792

PBN:	PADG_06579

ABE:

ARB_02211

TVE:

TRV_00460

AJE:	HCAG_02156

BGH:	BDBG_00313 BDBG_07612

PNO:	SNOG_02065

PTE:

PTT_16702

BZE:	COCCADRAFT_28711

BSC:	COCSADRAFT_264699

BOR:	COCMIDRAFT_95017

AALT:

CC77DRAFT_1022121 CC77DRAFT_1044489

ARAB:

EKO05_0006976

ZTR:	MYCGRDRAFT_72589 MYCGRDRAFT_72713

PFJ:	MYCFIDRAFT_142919 MYCFIDRAFT_77435

FFU:	CLAFUR5_08592

CBET:

CB0940_01869 CB0940_04755

BCOM:

BAUCODRAFT_63561

TML:	GSTUM_00008512001

SHS:	STEHIDRAFT_124754

ADL:	AURDEDRAFT_109597 AURDEDRAFT_144567 AURDEDRAFT_93828

GTR:	GLOTRDRAFT_123044

ABP:	AGABI1DRAFT80273(AGABI1DRAFT_80273)

ABV:	AGABI2DRAFT207832(AGABI2DRAFT_207832)

MPR:	MPER_10489 MPER_10728

MRR:	Moror_1658 Moror_1659 Moror_1672 Moror_3094 Moror_3605

E1B28_003449 E1B28_003452 E1B28_003467

CPUT:

CONPUDRAFT_113014

SLA:	SERLADRAFT_454411

ACAN:

ACA1_282210 ACA1_282220

» show all

Reference

1 [PMID:17493127]

Authors

Prado-Cabrero A, Estrada AF, Al-Babili S, Avalos J

Title

Identification and biochemical characterization of a novel carotenoid oxygenase: elucidation of the cleavage step in the Fusarium carotenoid pathway.

Journal

Mol Microbiol 64:448-60 (2007)
DOI:10.1111/j.1365-2958.2007.05665.x

Sequence

[ag:CAL90970 CAL90971]

Reference

2 [PMID:17610084]

Authors

Saelices L, Youssar L, Holdermann I, Al-Babili S, Avalos J

Title

Identification of the gene responsible for torulene cleavage in the Neurospora carotenoid pathway.

Journal

Mol Genet Genomics 278:527-37 (2007)
DOI:10.1007/s00438-007-0269-2

Reference

3 [PMID:18812228]

Authors

Estrada AF, Maier D, Scherzinger D, Avalos J, Al-Babili S

Title

Novel apocarotenoid intermediates in Neurospora crassa mutants imply a new biosynthetic reaction sequence leading to neurosporaxanthin formation.

Journal

Fungal Genet Biol 45:1497-505 (2008)
DOI:10.1016/j.fgb.2008.09.001

Other DBs

ExplorEnz - The Enzyme Database:

1.13.11.59

IUBMB Enzyme Nomenclature:

1.13.11.59

ExPASy - ENZYME nomenclature database:

1.13.11.59

BRENDA, the Enzyme Database:

1.13.11.59

DBGET integrated database retrieval system